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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0349 [new locus tag: SA_RS01995 ]
  • pan locus tag?: SAUPAN001907000
  • symbol: SA0349
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0349 [new locus tag: SA_RS01995 ]
  • symbol: SA0349
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 409131..410012
  • length: 882
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGATCAAGTTATGAGTATTATTTCATCGCTATTCGAACCATTAACTAAAATTGAAACA
    TATGAAAATATTGCAACTAAAATCGCTATGATTGTTATTTATATTATCGTAGCCCTCATA
    GTTATTAAAATACTGAATAAAATGATTGAACAGGGATTTAAGATTCAAAATAAAAGTAAA
    AAGAGTAACAAAAAGCGCTCTAAAACTTTAATATCTCTTGTTCAAAATGTAGTGAAGTAT
    ATCGTTTGGTTTATAGTTATTACGACGATTTTAAGTAAATTTGGCATTAGTGTTGAAGGT
    GTTATTGCAAGTGCTGGTGTCGTAGGTTTAGCAGTAGGTTTTGGTGCGCAGACCATTGTT
    AAAGACGTTATTACTGGATTTTTCATTATATTTGAAAGCCAATTTGATGTAGGTGATTAT
    GTTAAGATAAACAACGGTGGTACAACTGTAGCAGAGGGAACAGTTAAATCAATTGGACTC
    CGTTCAACACGTATTAATACAATATCTGGAGAACTTACAATCTTGCCAAATAGTAGCATG
    GGTGAAATAACGAATTACTCAATTACGAATGGAACAGCAATTGTTAAAATTCCAGTATCT
    GTAGATGAAAATATTGATAATGTTGAGAAGAAATTAAACAAATTATTTACATCTCTGCGT
    AGTAAATATTATTTATTTGTAAGTGATCCTGTTGTGATTGGTATTGATGCCTTTGAAGAT
    ACTAGAGTAATATTAAGGGTTTCAGCAGAAACAATACCAGGTGAAGGTTTCTCTGGTGCG
    CGTATTATTCGTAAAGAAGTACAAAAAATGTTTTTACAAGAAGGTATCAAGACACCACAA
    CCAATAATGACACCTTTTAATCATAGTGAAAACGGTGTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    882

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0349 [new locus tag: SA_RS01995 ]
  • symbol: SA0349
  • description: hypothetical protein
  • length: 293
  • theoretical pI: 9.86063
  • theoretical MW: 32206.5
  • GRAVY: 0.299317

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Potassium homeostasis Potassium efflux system KefA protein / Small-conductance mechanosensitive channel 
  • PFAM:
    no clan definedMS_channel; Mechanosensitive ion channel (PF00924; HMM-score: 137.3)
    DUF751; Protein of unknown function (DUF751) (PF05421; HMM-score: 15.7)
    Peptidase_MA (CL0126) DUF3810; Protein of unknown function (DUF3810) (PF12725; HMM-score: 11.3)
    no clan definedPhage_holin_6_1; Bacteriophage holin of superfamily 6 (Holin_LLH) (PF09682; HMM-score: 8.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.142
    • Ymax_pos: 56
    • Cmax: 0.152
    • Cmax_pos: 21
    • Smax: 0.28
    • Smax_pos: 48
    • Smean: 0.106
    • D: 0.128
  • predicted transmembrane helices (TMHMM): 3

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDQVMSIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKILNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVIASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGLRSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVDENIDNVEKKLNKLFTSLRSKYYLFVSDPVVIGIDAFEDTRVILRVSAETIPGEGFSGARIIRKEVQKMFLQEGIKTPQPIMTPFNHSENGV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]