From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0391 [new locus tag: SA_RS02245 ]
  • pan locus tag?: SAUPAN002121000
  • symbol: hsdM
  • pan gene symbol?: hsdM1
  • synonym:
  • product: type I site-specific deoxyribonuclease LldI subunit HsdM

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0391 [new locus tag: SA_RS02245 ]
  • symbol: hsdM
  • product: type I site-specific deoxyribonuclease LldI subunit HsdM
  • replicon: chromosome
  • strand: +
  • coordinates: 451000..452556
  • length: 1557
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCTATTACTGAAAAACAACGTCAGCAACAAGCTGAATTACATAAAAAATTATGGTCG
    ATTGCGAATGATTTAAGAGGGAATATGGATGCGAGTGAATTCCGTAATTACATTTTAGGC
    TTGATTTTCTATCGCTTCTTATCTGAAAAAGCGGAACAAGAATATGCAGATGCCTTGTCA
    GGTGAAGATATTACGTATCAAGAAGCATGGGCAGATGAAGAATATCGTGAAGACTTAAAA
    GCTGAATTAATTGATCAAGTCGGTTACTTCATTGAACCACAAGATTTATTCAGTGCGATG
    ATTCGTGAAATTGAAACGCAAGATTTCGATATCGAACATCTCGCAACGGCGATTCGTAAA
    GTTGAAACATCAACACTAGGTGAAGAAAGTGAAAATGACTTTATCGGACTGTTCAGCGAT
    ATGGACTTAAGTTCAACGCGACTAGGTAACAATGTCAAAGAACGTACTGCACTAATTTCC
    AAAGTTATGGTTAACCTTGACGACTTACCATTCGTTCACAGTGATATGGAAATTGATATG
    TTAGGTGATGCATACGAATTTCTTATCGGGCGCTTTGCGGCGACAGCAGGTAAAAAAGCA
    GGCGAGTTCTATACACCACAACAAGTATCTAAGATACTGGCGAAGATTGTCACAGACGGT
    AAAGATAAATTACGTCACGTGTATGACCCAACATGTGGTTCAGGTTCATTATTGTTACGT
    GTTGGTAAAGAAACGCAAGTGTATCGTTATTTCGGTCAAGAACGTAACAATACCACTTAC
    AACTTAGCGCGCATGAACATGTTATTACATGATGTGCGCTATGAAAACTTTGATATCCGT
    AATGATGATACGTTGGAAAATCCAGCTTTCTTAGGCAATACATTTGATGCGGTTATAGCG
    AACCCACCATACAGTGCGAAATGGACTGCAGATTCAAAATTTGAAAATGATGAACGCTTC
    AGTGGTTACGGCAAGCTTGCGCCAAAATCCAAAGCAGACTTTGCCTTTATTCAACATATG
    GTACATTACCTAGACGATGAAGGTACCATGGCAGTTGTACTCCCACATGGTGTCTTATTC
    CGTGGTGCCGCAGAAGGCGTCATTCGTCGTTATTTAATTGAAGAAAAGAACTACTTAGAA
    GCCGTGATTGGCTTACCAGTGAATATTTTCTATGGGACAAGTATTCCAACATGTATCTTA
    GTATTTAAAAAATGTCGCCAACAAGACGACAACGTATTATTTATCGATGCATCCAATGAT
    TTTGAAAAAGGAAAAAATCAAAACCATTTAAGCGATGCCCAAGTCGAACGTATTATTGAC
    ACATACAAGCGTAAAGAAACAATTGATAAATACAGTTACAGTGCGACATTACAAGAGATT
    GCCGATAACGATTACAACCTAAACATACCGAGGTATGTCGATACATTCGAAGAAGAAGCG
    CCAATTGATTTAGATCAAGTCCAACAAGATTTGAAAAATATCGACAAAGAAATCGCAGAA
    ATTGAACAAGAAATCAATGCATACCTGAAAGAACTTGGGGTGTTGAAAGATGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1557

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0391 [new locus tag: SA_RS02245 ]
  • symbol: HsdM
  • description: type I site-specific deoxyribonuclease LldI subunit HsdM
  • length: 518
  • theoretical pI: 4.38082
  • theoretical MW: 59471.2
  • GRAVY: -0.486873

Function[edit source | edit]

  • reaction:
    EC 3.1.21.3?  ExPASy
    Type I site-specific deoxyribonucleaseEndonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed
  • TIGRFAM:
    Genetic information processingDNA metabolismRestriction/modificationtype I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 466.6)
    Genetic information processingProtein fateProtein modification and repairprotein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 36.3)
    Genetic information processingDNA metabolismRestriction/modificationtype II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 34.2)
    Genetic information processingProtein fateProtein modification and repairmethyltransferase, HemK family (TIGR00536; HMM-score: 15.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprecorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 14.4)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationprotein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 14.1)
  • TheSEED:  
    DNA MetabolismDNA Metabolism - no subcategoryRestriction-Modification System Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72) 
    DNA Metabolism - no subcategoryType I Restriction-Modification Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72) 
  • PFAM:
    NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 371.3)
    no clan definedHsdM_N; HsdM N-terminal domain (PF12161; HMM-score: 75.3)
    NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 24.4)
    Eco57I; Eco57I restriction-modification methylase (PF07669; HMM-score: 22.5)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 16)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 14.7)
    UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 14.3)
    N6-adenineMlase; Probable N6-adenine methyltransferase (PF10237; HMM-score: 13.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.129
    • Ymax_pos: 59
    • Cmax: 0.138
    • Cmax_pos: 59
    • Smax: 0.232
    • Smax_pos: 7
    • Smean: 0.094
    • D: 0.115
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 15926109 NCBI
  • RefSeq: NP_373642 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPVNIFYGTSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]