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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0394 [new locus tag: SA_RS02260 ]
  • pan locus tag?: SAUPAN002130000
  • symbol: SA0394
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0394 [new locus tag: SA_RS02260 ]
  • symbol: SA0394
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 454848..456335
  • length: 1488
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGAGAAAATTTTAAATTACGTAAAATGAAAGTCGGTTTAGTATCAGTTGCAATTACT
    ATGTTATATATCATGACAAACGGACAAGCTGAAGCATCTGAAACTAATCAGAAGGTAAGT
    ACAAATCAAGAATCAAAAGCTGTTTCGCAGACTGAACAAAATTCCAAAGAAACAAAAGCG
    GCAGAGTCTTCTAAAAACTTTGTAAATTTAGATCCTATCAAACCAGGAGCTCAAAAAGTA
    ACAGGTACTACATTACCGAATCATATTATTTTACTTAATATAGATGGTAAAAGTGCTGAT
    TCAGTTGAAGGAGGATATGGTGATTTTATAACTGCAAATGACAAAGGAGAGTTTGAGTAT
    CCTTTAAATAATCGTAAAATTGTCCATAACCAAGAAATTGAGGTATCATCATCAAGCCCT
    GATTTAGGTGACGATGAAGAGGATGAAGAAGTAGAAGAATCTTCAACTGAAAAAGCTGGT
    GCTGAGGAAGAAAGTACAGATGCTAAAGCTACATATACAACACCACGATATGAAAAAGCG
    TATGAAATACCGAAAGAACAACTAAAAGAAAAAGATGGACACCACCAAGTTTTCATCGAA
    CCAATTACTGAAGGTTCAGGTATTATTAAAGGTCATACGTCTGTAAAAGGTAAAGTTGCT
    CTATCTATTAATAATAAATTTATTAATTTTGAAGAAAGAGCTAAAGGTGGAATTAGTAAA
    GAAGATACTAAAGCTAGTTCCGATGGTGTTTGGATGCCTATTAATGAAAAAGGATATTTT
    GATTTTGATTTCAAAAAAAATCCTTTTGATAACTTAGAGTTAAAGAAAAATGATGAAATC
    TCATTAACATTTGCACCTGATGATGAAGATGAAGCATTGAAGTCATTAATTTTCAAAACT
    AAAGTAACGAGCTTAGAAGATATCGATAAAGCAGAAACAAAATATGACCATACTAAAGTG
    GAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATATACATGTAGATGAAATTTACGGA
    AGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAACAAGGAACTAAAGAGATT
    ACAGGGAAGACTAAATTCGCGAATGCAGTAGTGAAAGTATATTCTGACTTAGGTGATGCG
    CAATTGTTCCCTGATATTCAAGTAGATGAAAATGGTAAATTTAGCTTTGATGCTGAAAAA
    GCTGGTTTCAGATTGCAAAATGGAGAAACATTGAATTTTGCAGTAGTTAAACCTATTACT
    GGTGACCTATTACATCAAGGATTTGTCTCTAAATATATTGATGTATATGAATCTCCGGAA
    GAAAAGAAAGAACGTGAATTTGAAGAAAAACTTGAAAACACGCCTGCATATCATAAATTA
    CATGGTGATAAAATTGTAGGATATGATGTTCAAGGTAATCCATCAACTTGGTTCTATCCA
    TTAGGTGAAAAGAAAGTTGAACGTACAACACCTAAATTAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1488

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0394 [new locus tag: SA_RS02260 ]
  • symbol: SA0394
  • description: hypothetical protein
  • length: 495
  • theoretical pI: 4.81306
  • theoretical MW: 55498.4
  • GRAVY: -0.795152

Function[edit source | edit]

  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 18.1)
  • TheSEED:  
    Hypothetical protein within Pathogenicity island SaPIn2 
  • PFAM:
    no clan definedYSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 15.5)
    FimbA (CL0450) Mfa_like_2; Fimbrillin-like (PF15415; HMM-score: 15.4)
    and 1 more
    no clan definedFAM176; FAM176 family (PF14851; HMM-score: 5.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 3
    • Predicted Cleavage Site: GQAEASET
  • SignalP: Signal peptide length 33 aa
    • Ymax: 0.824
    • Ymax_pos: 33
    • Cmax: 0.797
    • Cmax_pos: 33
    • Smax: 0.92
    • Smax_pos: 27
    • Smean: 0.837
    • D: 0.829
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASETNQKVSTNQESKAVSQTEQNSKETKAAESSKNFVNLDPIKPGAQKVTGTTLPNHIILLNIDGKSADSVEGGYGDFITANDKGEFEYPLNNRKIVHNQEIEVSSSSPDLGDDEEDEEVEESSTEKAGAEEESTDAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHTSVKGKVALSINNKFINFEERAKGGISKEDTKASSDGVWMPINEKGYFDFDFKKNPFDNLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLKDVKEDIHVDEIYGSLYHTEKGKGILDKQGTKEITGKTKFANAVVKVYSDLGDAQLFPDIQVDENGKFSFDAEKAGFRLQNGETLNFAVVKPITGDLLHQGFVSKYIDVYESPEEKKEREFEEKLENTPAYHKLHGDKIVGYDVQGNPSTWFYPLGEKKVERTTPKLEK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SaeR regulon
    SaeR(TF)important in VirulenceRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]