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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0412 [new locus tag: SA_RS02355 ]
  • pan locus tag?: SAUPAN002164000
  • symbol: SA0412
  • pan gene symbol?: mpsB
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0412 [new locus tag: SA_RS02355 ]
  • symbol: SA0412
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 472424..475129
  • length: 2706
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGACAACACAGTTAAATATCAATTCAGTCATTGAAAATGCGAAACGTGTTATTACACCA
    TTATCACCGATTTCGATTTTTGCAGCACGTAACCCCTGGGAAGGATTAGAAGCGGATACG
    TTTGAAGATGTCGCAAAATGGTTACGTGACGTTCGCGATGTGGATATTTTCCCAAATAAA
    GCATTAATAGAAAGCGCTGTGGCACGTGGTGAATTAGATGAAAGTGTCTTTAATCAACTT
    GTTACTGATATGTTACTTGAACATCACTACAATATCCCGCAACACTACATCAATCTTTAT
    ATTGATAACATTAAAACATTAAAAGACGTACCTGCATCATATATGAATCATTCAAATGTT
    GATGTTGTTGCTGATCTACTATTAGAAAAATCAAAACGTGATATGGCTGAATCATATCAT
    CACTATGATGTACGTCCGATGAGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGC
    GAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCG
    AGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAA
    CATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCT
    GAAATGACGATAGAGTCAGTATTAACTTATTTTTCAATAGATCAGGAAGACTACCAAGCT
    TATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCA
    CAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTC
    GAACAATTGCTAGTTGGTGATGAGTTTAAGTCAGTCACTAAAGATTGTGAAAGTAGATCG
    GAAAATTGGTTTAAGCAAACTGTTGCATCATTGTGTTACTACAGTGATATGCCTAGCGAT
    GTATTACTACAACATGACGTCAATGAAATTCAAACGTTTATTCATTTTGCAGCAACTATG
    AATAAAAATGTATTTAAAAATTTATGGCTAATTGCCTGGGAAATGACATACGAATCTCAG
    TTAAAACAAAAAATTAAAGCAGGTCATGAAAGTGTGGCGGGCGCATTAGATGTAAACCAA
    GTAAATGTCTCAGAAAATGATAACGCTAATCAGCCACATTCAGTATTGTTAAATGACACA
    CAAGCAGTTGATGAAAATAATAGCGAGCTAAATCAGGTGGGCACATCAACGAAAGCGCAA
    ATTGCATTTTGTATAGATGTTCGTTCAGAACCATTTCGTAGACATATCGAAGCAGCAGGG
    CCCTTTGAAACGATTGGTATTGCAGGTTTCTTTGGATTACCTATTCAAAAAGATGCCGTA
    GACGAACAATTCAAACATGATTCATTACCTGTCATGGTACCGCCGGCATATCGCATTAAA
    GAATTTGCAGACCGCTACGATATGAATGTTTATCGACAACAGCAACAGACAATGTCATCG
    ATGTTTTACACATTTAAATTGATGAAAAATAATGTTATGCCTAGTCTGTTATTGCCTGAA
    TTAAGTGGGCCATTTTTAAGCTTGAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGT
    CGCGCGTCTTTACAAAAAATAAAACAAAAATGGTTGAAAAAGCCTGAAACAAAGTTAACG
    ATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAA
    ATTGATTTCGCGTTACAAGCGTTGAAATTGATGGATTTAACCGAAGCATTTGCGCCGTTC
    GTTGTGTTAGCAGGTCATGCTAGTCATTCTCACAATAATCCACATCATGCATCACTTGAA
    TGTGGGGCTTGTGGTGGCGCATCAAGCGGTTTTAATGCTAAGTTATTAGCGATGATATGT
    AATCGTCCAAATGTCAGACAAGGATTAAAACAATCAGGTGTGTATATTCCAGAGACAACT
    GTTTTTGCGGCAGCAGAACATCATACGTCTACTGATACGTTGGCATGGGTATATGTGCCA
    GACACATTATCAGCTTTAGCTCTAGATGCATATGAATCATTGAATGACGCGATGCCGATG
    ATTTCTGAACAAGCGAATCGCGAACGTTTGGACAAACTGCCAACGATTGGTCGTGTGAAT
    CATCCAGTGGAAGAAGCACAGCGGTTTGCAAGTGATTGGAGTGAGGTACGTCCAGAATGG
    GGCTTGGCTAAAAATGCATCATTTATAATTGGACGACGCCAATTAACAAAAGGCATTGAT
    TTAGAAGGGCGGACATTTTTACACAATTATGATTGGCGTAAAGATAAAGATGGCAAATTG
    TTAAATACCATCATTTCTGGTCCGGCGCTTGTGGCACAATGGATTAATTTACAATATTAT
    GCGTCGACAGTTGCGCCGCATTTTTACGGAAGTGGGAATAAAGCGACACAAACCGTCACG
    TCAGGTGTTGGTGTCATGCAAGGTAATGCGAGTGATCTGATGTATGGCTTATCATGGCAA
    TCTGTTATGGCTGCTGATCGGACGATGTATCATTCGCCAATTCGTTTACTTGTCGTTATT
    CAGGCACCCGACTATGTTGTAGCTAGACTATTCGCGAATAATGAGCATTTCGCTAGGAAG
    GTGTCTAATCATTGGCTGCGTTTAATGAGCGTTAATGAGGAAGGGCGTTTTAAAAGTTGG
    ATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2706

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0412 [new locus tag: SA_RS02355 ]
  • symbol: SA0412
  • description: hypothetical protein
  • length: 901
  • theoretical pI: 6.12588
  • theoretical MW: 102576
  • GRAVY: -0.330633

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismAmino acid biosynthesisPyruvate family3-isopropylmalate dehydratase, small subunit (TIGR02084; EC 4.2.1.33; HMM-score: 11.6)
  • TheSEED:  
    Hypothetical transmembrane protein coupled to NADH-ubiquinone oxidoreductase chain 5 homolog 
  • PFAM:
    no clan definedDUF2309; Uncharacterized protein conserved in bacteria (DUF2309) (PF10070; HMM-score: 850.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.67
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.192
    • Ymax_pos: 29
    • Cmax: 0.214
    • Cmax_pos: 39
    • Smax: 0.326
    • Smax_pos: 28
    • Smean: 0.22
    • D: 0.203
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDVAKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYIDNIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLSEQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPNDPEMTIESVLTYFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHHFEQHLLTDYLAIRLVVEQLLVGDEFKSVTKDCESRSENWFKQTVASLCYYSDMPSDVLLQHDVNEIQTFIHFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQPHSVLLNDTQAVDENNSELNQVGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGFFGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLMKNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFDRTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGACGGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAAAEHHTSTDTLAWVYVPDTLSALALDAYESLNDAMPMISEQANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEWGLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGKLLNTIISGPALVAQWINLQYYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRLLVVIQAPDYVVARLFANNEHFARKVSNHWLRLMSVNEEGRFKSWI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Sonja Mayer, Wojtek Steffen, Julia Steuber, Friedrich Götz
The Staphylococcus aureus NuoL-like protein MpsA contributes to the generation of membrane potential.
J. Bacteriol.: 2015, 197(5);794-806
[PubMed:25448817] [WorldCat.org] [DOI] (I p)