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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0459 [new locus tag: SA_RS02650 ]
  • pan locus tag?: SAUPAN002242000
  • symbol: rplY
  • pan gene symbol?: rplY
  • synonym:
  • product: 50S ribosomal protein L25

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0459 [new locus tag: SA_RS02650 ]
  • symbol: rplY
  • product: 50S ribosomal protein L25
  • replicon: chromosome
  • strand: +
  • coordinates: 529681..530334
  • length: 654
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCTTCATTAAAGTCAATCATCCGTCAAGGTAAACAAACACGTTCAGATCTTAAACAA
    TTAAGAAAATCTGGTAAAGTACCAGCAGTAGTATACGGTTACGGTACTAAAAACGTGTCA
    GTTAAAGTTGATGAAGTAGAATTCATCAAAGTTATCCGTGAAGTAGGTCGTAACGGTGTT
    ATCGAATTAGGCGTTGGTTCTAAAACTATCAAAGTTATGGTTGCAGACTACCAATTCGAT
    CCACTTAAAAACCAAATTACTCACATTGACTTCTTAGCAATCAATATGAGTGAAGAACGT
    ACTGTTGAAGTACCAGTTCAATTAGTTGGTGAAGCAGTAGGCGCTAAAGAAGGCGGCGTA
    GTTGAACAACCATTATTCAACTTAGAAGTAACTGCTACTCCAGACAATATTCCAGAAGCA
    ATCGAAGTAGACATTACTGAATTAAACATTAACGACAGTTTAACTGTTGCTGATGTTAAA
    GTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTTGTAACAGTAGTTGCT
    CCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAA
    GAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    654

Protein[edit source | edit]

Protein Data Bank: 4WCE
Protein Data Bank: 4WFB
Protein Data Bank: 4WF9
Protein Data Bank: 4WFA

General[edit source | edit]

  • locus tag: SA0459 [new locus tag: SA_RS02650 ]
  • symbol: RplY
  • description: 50S ribosomal protein L25
  • length: 217
  • theoretical pI: 4.07608
  • theoretical MW: 23787.5
  • GRAVY: -0.341014

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationribosomal protein bL25, Ctc-form (TIGR00731; HMM-score: 168.4)
  • TheSEED:  
    Protein MetabolismProtein biosynthesisRibosome LSU bacterial LSU ribosomal protein L25p 
  • PFAM:
    no clan definedRibosomal_TL5_C; Ribosomal protein TL5, C-terminal domain (PF14693; HMM-score: 86.3)
    Ribosomal_L25p; Ribosomal L25p family (PF01386; HMM-score: 79.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.146
    • Ymax_pos: 18
    • Cmax: 0.109
    • Cmax_pos: 44
    • Smax: 0.276
    • Smax_pos: 11
    • Smean: 0.197
    • D: 0.166
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVDEVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTVEVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVKVTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]