From AureoWiki
Jump to: navigation, search

NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0521 [new locus tag: SA_RS03035 ]
  • pan locus tag?: SAUPAN002337000
  • symbol: sdrE
  • pan gene symbol?: sdrE
  • synonym:
  • product: Ser-Asp rich fibrinogen-binding, bone sialoprotein-binding protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0521 [new locus tag: SA_RS03035 ]
  • symbol: sdrE
  • product: Ser-Asp rich fibrinogen-binding, bone sialoprotein-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 612994..616419
  • length: 3426
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATTAACAGGGATAATAAAAAGGCAATAACAAAAAAGGGTATGATTTCAAATCGCTTA
    AACAAATTTTCGATTAGAAAGTATACTGTAGGAACTGCATCGATTTTAGTAGGTACGACA
    TTGATTTTTGGTCTAGGGAACCAAGAAGCTAAAGCTGCTGAAAACACTAGTACAGAAAAT
    GCGAAACAAGATGATGCAACGACTAGTGATAATAAAGAAGTAGTGTCGGAAACTGAAAAT
    AATTCGACAACAGAAAATGATTCAACAAATCCAATTAAGAAAGAAACAAATACTGATTCA
    CAACCAGAAGCTAAAGAAGAATCAACTACATCAAGTACTCAACAACAGCAAAATAACGTT
    ACAGCTACAACTGAAACTAAGCCTCAAAACATTGAAAAAGAAAATGTTAAACCTTCAACT
    GATAAAACTGCGACAGAAGATACATCTGTTATTTTAGAAGAGAAGAAAGCACCAAATTAT
    ACAAATAACGATGTAACTACAAAACCATCTACAAGTGAAATTCAAACAAAACCAACTACA
    CCTCAAGAATCTACAAATATTGAAAATTCACAACCGCAACCAACGCCTTCAAAAGTAGAC
    AATCAAGTTACAGATGCAACTAATCCAAAAGAACCAGTAAATGTGTCAAAAGAAGAACTT
    AAAAATAATCCTGAGAAATTAAAAGAATTAGTTAGAAATGATAACAATACAGATCGTTCA
    ACTAAACCAGTTGCTACAGCTCCAACAAGTGTTGCACCAAAACGATTAAATGCGAAAATG
    CGTTTTGCAGTTGCACAACCAGCAGCAGTTGCTTCAAATAATGTAAATGACTTAATTACA
    GTTACGAAACAGACGATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGAC
    GGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGAT
    ACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGAT
    CCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCCAAAGGAACATTTGATAAAGCG
    ACTAAGCAAATCACATATACATTTACAGATTATGTAGATAAATATGAAGATATAAAAGCA
    CGTTTAACTTTATACTCATATATTGATAAGCAAGCAGTACCTAATGAAACTAGTTTGAAT
    TTAACGTTTGCAACAGCAGGTAAAGAAACTAGCCAAAACGTTTCTGTTGATTATCAAGAC
    CCAATGGTTCATGGTGATTCAAACATTCAATCTATCTTTACAAAGTTAGATGAAAACAAA
    CAAACTATTGAACAACAAATTTATGTTAATCCTTTGAAAAAAACAGCAACTAACACTAAA
    GTTGATATAGCTGGTAGTCAAGTAGATGATTATGGAAATATTAAACTAGGAAATGGTAGT
    ACCATTATTGACCAAAATACAGAAATAAAAGTTTATAAAGTTAACCCTAATCAACAATTG
    CCTCAAAGTAATAGAATCTATGATTTTAGTCAATACGAAGATGTAACAAGTCAATTTGAT
    AATAAAAAATCATTTAGTAATAATGTAGCAACATTGGATTTTGGTGATATTAATTCAGCC
    TATATTATCAAAGTTGTTAGTAAATATACACCTACATCAGATGGCGAACTAGATATTGCT
    CAAGGTACTAGTATGAGAACAACTGATAAATATGGTTATTATAATTATGCAGGATATTCA
    AACTTCATCGTAACTTCTAATGACACTGGCGGTGGCGACGGTACTGTTAAACCTGAAGAA
    AAGTTATACAAAATTGGTGACTATGTATGGGAAGACGTTGATAAAGACGGTGTCCAAGGT
    ACAGATTCGAAAGAAAAGCCAATGGCAAACGTTTTAGTTACATTAACTTACCCGGACGGT
    ACTACAAAATCAGTAAGAACAGATGCTAACGGTCATTATGAATTCGGTGGTTTGAAAGAC
    GGAGAAACTTATACAGTTAAATTCGAAACGCCAGCTGGATATCTTCCAACAAAAGTAAAT
    GGAACAACTGATGGTGAAAAAGACTCAAATGGTAGTTCTATAACTGTTAAAATTAATGGT
    AAAGATGATATGTCTTTAGACACTGGTTTTTATAAAGAACCTAAATATAATCTTGGTGAC
    TATGTATGGGAAGATACAAATAAAGATGGTATCCAAGATGCTAATGAACCTGGTATCAAA
    GATGTTAAGGTTACATTAAAAGATAGTACTGGAAAAGTTATTGGTACAACTACTACTGAT
    GCCTCGGGTAAATATAAATTTACAGATTTAGATAATGGTAACTATACAGTAGAATTTGAA
    ACACCAGCAGGTTACACGCCAACGGTTAAAAATACTACAGCTGAAGATAAAGATTCTAAT
    GGTTTAACAACAACAGGTGTCATTAAAGATGCAGATAATATGACATTAGACAGTGGTTTC
    TATAAAACACCAAAATACAGTTTAGGTGATTATGTTTGGTACGACAGTAATAAAGACGGT
    AAACAAGATTCAACTGAAAAAGGTATCAAAGATGTTAAAGTTACTTTATTAAATGAAAAA
    GGCGAAGTAATTGGAACAACTAAAACAGATGAAAATGGTAAATATCGTTTCGATAATTTA
    GATAGCGGTAAATACAAAGTTATTTTTGAAAAGCCTGCTGGCTTAACACAAACAGTTACA
    AATACAACTGAAGATGATAAAGATGCCGATGGTGGCGAAGTTGACGTAACAATTACGGAT
    CATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGTGATTCA
    GACTCAGACAGTGATTCAGACTCAGACAGCGACTCAGATTCAGACAGTGATTCAGACTCA
    GATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGC
    GACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCA
    GATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCA
    GATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGC
    GACTCAGATTCGGACAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCA
    GACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGACTCAGATAGCGATTCGGACTCG
    GATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAA
    GCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTT
    GGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAAC
    AAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3426

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0521 [new locus tag: SA_RS03035 ]
  • symbol: SdrE
  • description: Ser-Asp rich fibrinogen-binding, bone sialoprotein-binding protein
  • length: 1141
  • theoretical pI: 4.03874
  • theoretical MW: 124026
  • GRAVY: -1.06705

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 50.2)
    Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 16.4)
    Por secretion system C-terminal sorting domain (TIGR04183; HMM-score: 16.3)
    MetabolismEnergy metabolismOtherprotocatechuate 3,4-dioxygenase, beta subunit (TIGR02422; EC 1.13.11.3; HMM-score: 12.4)
  • TheSEED:  
    Virulence, Disease and DefenseAdhesionAdhesins in Staphylococcus Adhesin of unknown specificity SdrE, similar to bone sialoprotein-binding protein Bbp 
  • PFAM:
    Transthyretin (CL0287) SdrD_B; SdrD B-like domain (PF17210; HMM-score: 250.6)
    CarboxypepD_reg; Carboxypeptidase regulatory-like domain (PF13620; HMM-score: 105.1)
    Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 102.3)
    E-set (CL0159) MG1; Alpha-2-macroglobulin MG1 domain (PF11974; HMM-score: 48.5)
    no clan definedYSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 45.4)
    Transthyretin (CL0287) MetallophosN; N terminal of Calcineurin-like phosphoesterase (PF16371; HMM-score: 44.1)
    no clan definedGram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 42.7)
    Transthyretin (CL0287) CarbopepD_reg_2; CarboxypepD_reg-like domain (PF13715; HMM-score: 29.3)
    DUF2012; Protein of unknown function (DUF2012) (PF09430; HMM-score: 20.4)
    DUF2606; Protein of unknown function (DUF2606) (PF10794; HMM-score: 18.1)
    DUF1416; Protein of unknown function (DUF1416) (PF07210; HMM-score: 17.7)
    no clan definedCompInhib_SCIN; Staphylococcal complement inhibitor SCIN (PF11546; HMM-score: 16.8)
    GOLD-like (CL0521) EMP24_GP25L; emp24/gp25L/p24 family/GOLD (PF01105; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: found LPxTG motif :LPETG
  • SignalP: Signal peptide length 53 aa
    • Ymax: 0.731
    • Ymax_pos: 53
    • Cmax: 0.791
    • Cmax_pos: 53
    • Smax: 0.857
    • Smax_pos: 49
    • Smean: 0.453
    • D: 0.623
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFGLGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENDSTNPIKKETNTDSQPEAKEESTTSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNYTNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKEELKNNPEKLKELVRNDNNTDRSTKPVATAPTSVAPKRLNAKMRFAVAQPAAVASNNVNDLITVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKARLTLYSYIDKQAVPNETSLNLTFATAGKETSQNVSVDYQDPMVHGDSNIQSIFTKLDENKQTIEQQIYVNPLKKTATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNPNQQLPQSNRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPAGYLPTKVNGTTDGEKDSNGSSITVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTAEDKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

T J Foster, M Höök
Surface protein adhesins of Staphylococcus aureus.
Trends Microbiol.: 1998, 6(12);484-8
[PubMed:10036727] [WorldCat.org] (P p)