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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0549 [new locus tag: SA_RS03175 ]
  • pan locus tag?: SAUPAN002373000
  • symbol: mvaK2
  • pan gene symbol?: mvaK2
  • synonym:
  • product: phosphomevalonate kinase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA0549 [new locus tag: SA_RS03175 ]
  • symbol: mvaK2
  • product: phosphomevalonate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 642673..643749
  • length: 1077
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGATTCAGGTCAAAGCACCCGGAAAACTTTATATTGCTGGAGAATATGCTGTAACAGAA
    CCAGGATATAAATCTGTACTTATTGCGTTAGATCGTTTTGTAACTGCTACTATTGAAGAA
    GCAGACCAATATAAAGGTACCATTCATTCAAAAGCATTACATCATAACCCAGTTACATTT
    AGTAGAGATGAAGATAGTATTGTCATTTCAGATCCACATGCAGCAAAACAATTAAATTAT
    GTGGTCACAGCTATTGAAATATTTGAACAATACGCGAAAAGTTGCGATATAGCGATGAAG
    CATTTTCATCTGACTATTGATAGTAATTTAGATGATTCAAATGGTCATAAATATGGATTA
    GGTTCAAGTGCAGCAGTACTTGTGTCAGTTATAAAAGTATTAAATGAATTTTATGATATG
    AAGTTATCTAATTTATACATTTATAAACTAGCAGTGATTGCAAATATGAAGTTACAAAGT
    TTAAGTTCATGCGGAGATATTGCTGTGAGTGTATATAGTGGATGGTTAGCGTATAGTACT
    TTTGATCATGAATGGGTTAAGCATCAAATTGAAGATACTACGGTTGAAGAAGTTTTAATC
    AAAAACTGGCCTGGATTGCACATCGAACCATTACAAGCACCTGAAAATATGGAAGTACTT
    ATCGGTTGGACTGGCTCACCGGCGTCATCACCACACTTTGTTAGCGAAGTGAAACGTTTG
    AAATCAGATCCTTCATTTTACGGTGACTTCTTAGAAGATTCACATCGTTGTGTTGAAAAG
    CTTATTCATGCTTTTAAAACAAATAACATTAAAGGTGTGCAAAAGATGGTGCGTCAGAAT
    CGTACAATTATTCAACGTATGGATAAAGAAGCTACAGTTGATATAGAAACTGAAAAGCTA
    AAATATTTGTGTGATATTGCTGAAAAGTATCACGGTGCATCTAAAACATCAGGCGCTGGT
    GGTGGAGACTGTGGTATTACAATTATCAATAAAGATGTAGATAAAGAAAAAATTTATGAT
    GAATGGACAAAACATGGTATTAAACCATTAAAATTTAATATTTATCATGGGCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1077

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA0549 [new locus tag: SA_RS03175 ]
  • symbol: MvaK2
  • description: phosphomevalonate kinase
  • length: 358
  • theoretical pI: 6.50932
  • theoretical MW: 40216.6
  • GRAVY: -0.272626

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Central intermediary metabolism Other phosphomevalonate kinase (TIGR01220; EC 2.7.4.2; HMM-score: 584.7)
    and 5 more
    Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 92.4)
    Metabolism Central intermediary metabolism Other phosphomevalonate kinase (TIGR01219; EC 2.7.4.2; HMM-score: 59.6)
    Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 22.1)
    Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 13.7)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 12.8)
  • TheSEED  :
    • Phosphomevalonate kinase (EC 2.7.4.2)
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids  Phosphomevalonate kinase (EC 2.7.4.2)
    and 1 more
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis  Phosphomevalonate kinase (EC 2.7.4.2)
  • PFAM:
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 48.6)
    no clan defined GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 42.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004989
    • TAT(Tat/SPI): 0.000323
    • LIPO(Sec/SPII): 0.000758
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQYKGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHFHLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQSLSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENMEVLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKMVRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVDKEKIYDEWTKHGIKPLKFNIYHGQ

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]