From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0581 [new locus tag: SA_RS03350 ]
  • pan locus tag?: SAUPAN002497000
  • symbol: SA0581
  • pan gene symbol?: mnhD2
  • synonym:
  • product: monovalent cation/H+ antiporter subunit D

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0581 [new locus tag: SA_RS03350 ]
  • symbol: SA0581
  • product: monovalent cation/H+ antiporter subunit D
  • replicon: chromosome
  • strand: +
  • coordinates: 673120..674616
  • length: 1497
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCTAAGTAACTTATTGATTTTACCAATGTTATTACCATTCCTTTGTGCCTTAATCCTT
    GTATTTTTAAAAAATAATGATCGTATTTCTAAATATTTATACTTAGGTACAATGACTATC
    ACCACAATTATTTCATTAATGCTATTAATTTATGTTCAGCGTCACCGTCCAATTACGCTA
    GACTTTGGAGGATGGTCAGCGCCCTTTGGTATACAGTTTTTAGGAGATTCTTTAAGTTTG
    ATTATGGTTACAACCGCTTCGTTTGTGATTACTTTAATTATGGCATACGGATTTGGGCGT
    GGCGAACATAAAGCAAATCGTTATCACTTGCCATCGTTCATATTATTTTTAAGTGTTGGC
    GTGATAGGCTCTTTTCTAACATCAGATTTATTTAATTTATACGTCATGTTTGAAATTATG
    TTACTAGCGTCATTTGTACTCATTACACTTGGACAATCTGTAGAACAATTACGTGCTGCA
    ATTATTTATGTTGTCTTGAATATTATTGGTTCATGGCTATTCTTATTAGGTATAGGTTTA
    CTTTATAAAACAGTAGGTACATTAAACTTTTCACATATTGCAATGCGTTTGAATGACATG
    GGAGATAATCGCACTGTTACAATGATTTCATTAATCTTCTTAGTCGCATTTAGTGCGAAA
    GCAGCGCTGGTCCTTTTTATGTGGCTACCCAAAGCCTACGCTGTGTTAAATACTGAGCTT
    GCAGCATTATTTGCAGCGTTAATGACCAAAGTAGGGGCCTATGCATTAATTCGATTCTTC
    ACTTTACTATTTGATCAACATAATGATCTCATACATCCATTGCTAGCAACTATGGCTGCT
    ATAACTATGGTCATCGGCGCTATAGGTGTCATTGCTTATAAAGATATTAAAAAGATTGCA
    GCTTACCAAGTCATAATCTCAATAGGATTTATCATTTTAGGTTTAGGAACAAACACGTTT
    GCAGGTATTAATGGTGCAATATTTTATTTGGTAAATGACATTGTTGTAAAAACATTGCTA
    TTCTTTATTATTGGTAGTTTAGTTTACATTACAGGCTATCGACAATATCAATATTTGAAT
    GGCTTAGCTAAAAAAGAACCTTTATTTGGAGTTGCGTTTATTATAATGATTTTTGCTATT
    GGCGGCGTGCCTCCATTTAGTGGCTTTCCGGGGAAAGTACTTATTTTCCAAGGTGCATTG
    CAAAATGGCAATTATATTGGACTAGCGTTAATGATTATTACTAGTCTAATTGCAATGTAC
    AGTTTATTTAGGATATTTTTTTATATGTATTTTGGAGATAAAGATGGGGAGGAAGTTAAT
    TTTAAGAAAATCCCGCTATATCGAAAAAGAATTTTAAGTATTTTAGTAGTTGTGGTTATC
    GCAATCGGAATTGCTGCACCTGTTGTGTTAAATGTTACAAGTGATGCAACTGAGTTGAAC
    ACGAGTGATCAATTATATCAAAAACTTGTAAATCCGCATTTGAAAGGAGAGGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1497

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0581 [new locus tag: SA_RS03350 ]
  • symbol: SA0581
  • description: monovalent cation/H+ antiporter subunit D
  • length: 498
  • theoretical pI: 9.6341
  • theoretical MW: 55186.9
  • GRAVY: 0.920482

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmulticomponent K+:H+antiporter (TIGR00944; HMM-score: 366.7)
    MetabolismEnergy metabolismElectron transportproton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 203.2)
    MetabolismEnergy metabolismElectron transportproton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 175.1)
    MetabolismEnergy metabolismElectron transportproton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 130.4)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmonovalent cation:proton antiporter (TIGR00940; HMM-score: 93.8)
    NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 67.7)
  • TheSEED:  
    Membrane TransportUni- Sym- and AntiportersSodium Hydrogen Antiporter Na(+) H(+) antiporter subunit D (TC 2.A.63.1.3) 
  • PFAM:
    ComplexI-N (CL0425) Proton_antipo_M; Proton-conducting membrane transporter (PF00361; HMM-score: 189.6)
    no clan definedProton_antipo_N; NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus (PF00662; HMM-score: 17.5)
    DUF1490; Protein of unknown function (DUF1490) (PF07371; HMM-score: 14)
    DUF2749; Protein of unknown function (DUF2749) (PF10907; HMM-score: 10.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.208
    • Ymax_pos: 12
    • Cmax: 0.119
    • Cmax_pos: 31
    • Smax: 0.507
    • Smax_pos: 3
    • Smean: 0.431
    • D: 0.295
  • predicted transmembrane helices (TMHMM): 14

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTIISLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGEHKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAAIIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFSAKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLATMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDIVVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGKVLIFQGALQNGNYIGLALMIITSLIAMYSLFRIFFYMYFGDKDGEEVNFKKIPLYRKRILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]