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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0732 [new locus tag: SA_RS04165 ]
  • pan locus tag?: SAUPAN002711000
  • symbol: SA0732
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0732 [new locus tag: SA_RS04165 ]
  • symbol: SA0732
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 839200..839658
  • length: 459
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATC
    GTATTCTCAATATTATTGATTATTCATTACTTTGTTTCGTTGGATAATGCGACTGCCAAA
    GCATTACTTAATTTAACGAATCAAAACACTTCAGATAAAGCGATTGATTACATTTTAAAC
    AGCTTTAGATTCACTGGTATTATGTATATTTTGGCTTATCTAGCAGGCTTCATCACTTTT
    TGGAATCGACATACTTATGTGTGGTGGTTTATGTTTGCAGTTTATGTATCAAATAGTTTG
    TTTACGTTGATTAATTTATCAATCACAATTCAAGCAATAAAAGCTGCACACGGTGCGTAC
    TTAACATTGCCAATTTTAATCGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTT
    GTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAG
    60
    120
    180
    240
    300
    360
    420
    459

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0732 [new locus tag: SA_RS04165 ]
  • symbol: SA0732
  • description: hypothetical protein
  • length: 152
  • theoretical pI: 10.3529
  • theoretical MW: 17242.3
  • GRAVY: 0.698026

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    FIG01108117: hypothetical protein 
  • PFAM:
    Tetraspannin (CL0347) DUF4064; Protein of unknown function (DUF4064) (PF13273; HMM-score: 15.3)
    no clan definedDUF2721; Protein of unknown function (DUF2721) (PF11026; HMM-score: 10.7)
    DUF373; Domain of unknown function (DUF373) (PF04123; HMM-score: 8.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.267
    • Ymax_pos: 40
    • Cmax: 0.199
    • Cmax_pos: 40
    • Smax: 0.565
    • Smax_pos: 37
    • Smean: 0.254
    • D: 0.262
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLNLTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFTLINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol. Microbiol.: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] (P p)

Relevant publications[edit source | edit]