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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0744 [new locus tag: SA_RS04235 ]
  • pan locus tag?: SAUPAN002796000
  • symbol: ssp
  • pan gene symbol?: emp
  • synonym:
  • product: extracellular ECM and plasma binding protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0744 [new locus tag: SA_RS04235 ]
  • symbol: ssp
  • product: extracellular ECM and plasma binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 853529..854551
  • length: 1023
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAAAGAAATTATTAGTTTTAACTATGAGCACGCTATTTGCTACACAACTTATCAAT
    TCAAATCACGCTAAAGCATCAGTGACAGAGAGTGTTGACAAAAAATTTGTAGTTCCAGAA
    TCAGGAATTAATAAAATTATTCCAGCTTACGATGAATTTAAGAATTCGCCAAAAGTAAAT
    GTTAGTAATTTAACTGACAATAAAAACTTTGTAGTTTCTGAAGACAAATTGAATAAGATT
    GTAGATTCATCGGCAGCTAGTAAAATTGTAGATAAAAACTTTGCCGTACCAGAATCAAAG
    TTAGGAAACATTGTACCAGAGTACAAAGAAATCAATAATCGCGTGAATGTAGCAACAAAC
    AATCCAGCTTCACAACAAGTTGATAAGCATTTTGTTGCTAAAGGCCCAGAAGTAAATAGA
    TTTATTACGCAAAACAAAGTAAACCACCACTTCATTACTACGCAAACCCACTACAAGAAA
    GTTATTACTTCATACAAATCAACACATGTACATAAACATGTAAATCATGCAAAGGATTCT
    ATTAATAAACACTTTATTGTTAAACCATCAGAATCGCCTAGATATACACATCCATCTCAA
    TCTTTAATTATCAAGCATCATTTTGCAGTTCCTGGATATCACGCGCATAAATTTGTTACA
    CCAGGGCATGCTAGCATTAAAATTAATCACTTTTGTGTTGTGCCACAAATAAATAGTTTC
    AAGGTAATTCCACCATATGGTCACAATTCACATCGTATGCATGTACCAAGTTTCCAAAAT
    AACACAACAGCAACACATCAAAATGCTAAAGTAAATAAAGCATATGACTATAAATACTTC
    TATTCTTATAAAGTAGTTAAAGGTGTGAAGAAATATTTCTCATTTTCACAATCAAATGGT
    TATAAAATTGGGAAACCATCATTAAATATCAAAAATGTAAATTATCAATATGCTGTTCCA
    AGTTATAGCCCTACACACTACGTTCCTGAATTTAAGGGTAGCTTACCAGCACCACGAGTA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1023

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0744 [new locus tag: SA_RS04235 ]
  • symbol: Ssp
  • description: extracellular ECM and plasma binding protein
  • length: 340
  • theoretical pI: 10.4459
  • theoretical MW: 38494.6
  • GRAVY: -0.547353

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Virulence, Disease and DefenseAdhesionAdhesins in Staphylococcus Extracellular ECM and plasma binding protein Emp 
  • PFAM:

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 9.94
    • Extracellular Score: 0.06
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: NHAKASVT
  • SignalP: Signal peptide length 27 aa
    • Ymax: 0.751
    • Ymax_pos: 27
    • Cmax: 0.66
    • Cmax_pos: 27
    • Smax: 0.924
    • Smax_pos: 15
    • Smean: 0.878
    • D: 0.801
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKKLLVLTMSTLFATQLINSNHAKASVTESVDKKFVVPESGINKIIPAYDEFKNSPKVNVSNLTDNKNFVVSEDKLNKIVDSSAASKIVDKNFAVPESKLGNIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKKVITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKFVTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYDYKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGSLPAPRV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

M Hussain, K Becker, C von Eiff, J Schrenzel, G Peters, M Herrmann
Identification and characterization of a novel 38.5-kilodalton cell surface protein of Staphylococcus aureus with extended-spectrum binding activity for extracellular matrix and plasma proteins.
J. Bacteriol.: 2001, 183(23);6778-86
[PubMed:11698365] [WorldCat.org] [DOI] (P p)