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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0849 [new locus tag: SA_RS04815 ]
  • pan locus tag?: SAUPAN003137000
  • symbol: SA0849
  • pan gene symbol?: opp-3A
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0849 [new locus tag: SA_RS04815 ]
  • symbol: SA0849
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 962060..963715
  • length: 1656
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACGAGAAAATTTAGAACACTTATTTTAATTTTGATTGCTACAATTGCATTAAGTGGT
    TGTGCTAATGACGATGGTATTTATTCAGATAAAGGTCAAGTATTCAGAAAAATTTTGTCA
    TCAGACTTAACATCCCTTGATACATCATTAATAACGGATGAAATATCTTCTGAAGTGACT
    GCGCAAACATTCGAAGGTTTATACACATTAGGAAAAGGTGACAAACCGGTGTTAGGTGTT
    GCGAAAGCTTTTCCTGAAAAGAGTAAAGATGGTAAAACTTTAAAGGTTAAATTAAGAAGC
    GATGCTAAATGGAGCAATGGTGACAAAGTGACTGCACAAGACTTTGTTTATGCTTGGAGA
    AAAACAGTTGACCCTAAAACAGGTTCTGAATTTGCATACATTATGGGGGACATTAAAAAT
    GCGAGTGATATTAGTACTGGTAAGAAACCTGTAGAGCAATTAGGTATCAAAGCATTAAAT
    GATGAAACATTACAAATTGAATTAGAAAAGCCGGTTCCATATATTAATCAATTATTAGCA
    CTTAATACCTTTGCACCTCAAAATGAAAAAGTTGCCAAAAAATATGGTAAAAATTACGGT
    ACGGCAGCTGATAGAGCGGTATACAATGGTCCATTTAAAGTTGATGATTGGAAACAAGAA
    GATAAAACCTTACTATCTAAAAATCAGTATTATTGGGATAAAAAGAATGTAAAATTAGAT
    AAAGTGAATTATAAAGTTATTAAAGACTTACAAGCCGGTGCATCATTGTATGATACTGAA
    TCAGTAGATGACGCAGTTATTACTGCAGATCAAGTAAATAAATATAAAGACAACAAAGGA
    TTAAACTTTGTGTTAACGTCTGGGACATTTTTTGTAAAAATGAATGAAAAACAATATCCT
    GATTTTAAAAACAAAAATTTAAGATTGGCTATCGCACAAGCAATAGATAAAAAAGGATAC
    GTTGATTCAGTGAAAAACAATGGCTCAATTCCTTCCGATACACTAACAGCCAAAGGAATT
    GCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAAT
    CCTAAAGAAGCAAGAGCACACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTG
    ACATTTTCAATGAACACAGAAGATACACCAGATGCAAAAATATCTGCTGAATATATCAAA
    TCGCAAGTTGAGAAAAATTTACCAGGAGTTACTTTGAAAATTAAGCAATTACCGTTTAAA
    CAAAGAGTATCACTAGAACTGAGTAACAATTTTGAAGCATCACTTAGTGGTTGGTCTGCA
    GATTACCCTGATCCTATGGCTTATTTAGAAACAATGACCACAGGTAGCGCACAAAATAAT
    ACAGACTGGGGTAATAAAGAATATGATCAATTACTTAAAGTAGCAAGAACCAAATTGGCA
    CTTCAACCGAACGAACGATATGAAAACTTGAAAAAAGCAGAAGAAATGTTCCTAGGAGAT
    GCACCGGTAGCACCAATTTATCAAAAAGGTGTTGCACATTTAACAAATCCTCAAGTAAAA
    GGATTAATTTACCATAAATTTGGTCCAAATAACTCACTTAAACATGTATATATTGATAAA
    TCGATAGATAAAGAAACAGGTAAGAAGAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1656

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0849 [new locus tag: SA_RS04815 ]
  • symbol: SA0849
  • description: hypothetical protein
  • length: 551
  • theoretical pI: 9.72302
  • theoretical MW: 61557.6
  • GRAVY: -0.642105

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsnickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 68.3)
  • TheSEED:  
    Membrane TransportABC transportersABC transporter oligopeptide (TC 3.A.1.5.1) Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1) 
    Regulation and Cell signalingRegulation and Cell signaling - no subcategorySex pheromones in Enterococcus faecalis and other Firmicutes Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1) 
  • PFAM:
    PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 248.5)
    LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 12.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 9.21
    • Extracellular Score: 0.78
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: IALSGCA
  • SignalP: no predicted signal peptide
    • Ymax: 0.285
    • Ymax_pos: 23
    • Cmax: 0.154
    • Cmax_pos: 30
    • Smax: 0.788
    • Smax_pos: 9
    • Smean: 0.628
    • D: 0.419
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLTSLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDAKWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALNDETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWKQEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYKDNKGLNFVLTSGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDTLTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDAKISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLETMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQKGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Aurelia Hiron, Elise Borezée-Durant, Jean-Christophe Piard, Vincent Juillard
Only one of four oligopeptide transport systems mediates nitrogen nutrition in Staphylococcus aureus.
J. Bacteriol.: 2007, 189(14);5119-29
[PubMed:17496096] [WorldCat.org] [DOI] (P p)