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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0865 [new locus tag: SA_RS04895 ]
  • pan locus tag?: SAUPAN003178000
  • symbol: ppnK
  • pan gene symbol?: ppnK
  • synonym:
  • product: inorganic polyphosphate/ATP-NAD kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0865 [new locus tag: SA_RS04895 ]
  • symbol: ppnK
  • product: inorganic polyphosphate/ATP-NAD kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 978836..979645
  • length: 810
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCGTTATACAATTTTAACTAAAGGTGACTCCAAGTCTAATGCCTTAAAGCATAAAATG
    ATGAACTATATGAAAGATTTTCGCATGATTGAGGATAGTGAAAATCCTGAAATTGTTATT
    TCAGTTGGTGGTGATGGTACATTACTACAAGCATTCCATCAGTATAGCCACATGTTATCA
    AAAGTGGCATTTGTTGGAGTTCATACAGGTCATTTAGGATTTTATGCGGATTGGTTACCT
    CATGAAGTTGAAAAATTAATCATCGAAATTAATAATTCAGAGTTTCAGGTCATTGAATAT
    CCATTGCTTGAAATTATTATGAGATACAACGACAACGGCTATGAAACAAGGTATTTAGCA
    TTAAATGAAGCAACGATGAAAACTGAAAATGGCTCAACACTTGTTGTGGATGTTAACTTA
    AGAGGGAAACACTTTGAGCGATTTAGAGGCGATGGATTATGTGTATCAACACCTTCGGGT
    TCAACGGCTTATAACAAAGCGCTAGGTGGCGCACTGATACATCCTTCACTTGAAGCAATG
    CAAATTACAGAAATTGCCTCAATAAATAATCGTGTGTTTAGAACGGTAGGATCACCACTT
    GTATTACCAAAGCATCATACATGTTTAATATCACCAGTTAATCATGATACCATTAGAATG
    ACGATAGATCATGTTAGTATCAAACATAAAAATGTTAATTCAATACAATACCGTGTAGCA
    AATGAAAAAGTGAGGTTTGCACGTTTTAGACCATTCCCGTTCTGGAAACGTGTACACGAT
    TCTTTCATATCAAGTGATGAAGAACGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    810

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0865 [new locus tag: SA_RS04895 ]
  • symbol: PpnK
  • description: inorganic polyphosphate/ATP-NAD kinase
  • length: 269
  • theoretical pI: 8.264
  • theoretical MW: 30769
  • GRAVY: -0.310037

Function[edit source | edit]

  • reaction:
    EC 2.7.1.23?  ExPASy
    NAD+ kinaseATP + NAD+ = ADP + NADP+
  • TIGRFAM:
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsNAD and NADPNAD and NADP cofactor biosynthesis global NAD kinase (EC 2.7.1.23) 
    CBSS-222523.1.peg.1311 NAD kinase (EC 2.7.1.23) 
  • PFAM:
    PFK (CL0240) NAD_kinase; ATP-NAD kinase (PF01513; HMM-score: 92.1)
    CDA (CL0109) LmjF365940-deam; A distinct subfamily of CDD/CDA-like deaminases (PF14421; HMM-score: 11.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: a divalent metal cation
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.152
    • Ymax_pos: 11
    • Cmax: 0.108
    • Cmax_pos: 52
    • Smax: 0.292
    • Smax_pos: 1
    • Smean: 0.226
    • D: 0.181
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGGDGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPLLEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSGSTAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTIRMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]