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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1119 [new locus tag: SA_RS06330 ]
  • pan locus tag?: SAUPAN003577000
  • symbol: spoIIIE
  • pan gene symbol?: ftsK
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1119 [new locus tag: SA_RS06330 ]
  • symbol: spoIIIE
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1270705..1273071
  • length: 2367
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    TTGGCACAAGCAAAAAAGAAATCGACAGCTAAGAAAAAAACAGCATCAAAAAAAAGAACA
    AATTCAAGGAAAAAGAAGAATGATAATCCGATACGTTATGTCATAGCTATTTTAGTAGTT
    GTATTAATGGTGTTGGGTGTTTTCCAATTAGGAATAATCGGTCGTCTAATTGACAGCTTC
    TTTAATTATTTATTTGGGTACAGTAGATATTTAACATATATTTTAGTACTCTTAGCAACT
    GGTTTTATTACATACTCTAAACGTATTCCTAAAACTAGACGAACGGCTGGTTCGATTGTA
    TTGCAAATTGCATTGCTATTTGTATCACAGTTAGTTTTTCATTTTAATAGTGGTATCAAA
    GCTGAAAGAGAACCTGTACTTTCTTATGTATATCAGTCATACCAACACAGTCATTTTCCA
    AATTTTGGTGGCGGTGTATTAGGTTTTTATTTATTAGAGTTAAGCGTACCTTTAATTTCA
    TTATTTGGTGTATGTATTATTACTATTTTATTATTATGCTCAAGTGTTATTTTATTAACA
    AACCATCAACATCGTGATGTTGCAAAAGTTGCACTGGAAAATATAAAAGCTTGGTTTGGT
    TCATTTAATGAAAAAATGTCGGAAAGAAACCAAGAAAAACAATTGAAGCGTGAAGAAAAA
    GCGAGACTTAAAGAAGAACAAAAGGCACGTCAAAATGAACAGCCACAAATAAAAGATGTG
    AGTGATTTTACGGAAGTGCCTCAAGAAAGAGATATTCCAATTTATGGGCATACTGAAAAT
    GAAAGTAAAAGCCAGTGTCAACCAAGTCGAAAAAAACGAGTGTTTGATGCAGAGAATAGT
    TCGAATAACATCGTAAATCATCAAGCAGATCAGCAAGAGCAATTAACAGAACAAACTCAT
    AACAGTGTTGAAAGTGAAAACACTATTGAAGAAGCTGGTGAAGTTACGAATGTATCGTAT
    GTTGTTCCACCGTTAACTTTACTTAATCAACCTGCAAAACAAAAAGCAACATCTAAAGCT
    GAAGTACAACGTAAAGGACAAGTACTAGAGAATACATTAAAAGATTTTGGGGTAAATGCA
    AAAGTGACACAAATTAAAATTGGTCCTGCAGTAACTCAATATGAAATTCAACCAGCTCAA
    GGGGTTAAAGTGAGTAAAATTGTAAACTTGCATAATGATATTGCATTAGCTTTAGCAGCA
    AAAGATGTTAGAATCGAAGCACCAATACCTGGTCGCTCTGCAGTAGGTATTGAAGTGCCA
    AATGAGAAAATTTCATTAGTTTCACTAAAAGAAGTTTTAGATGAAAAATTCCCGTCTAAT
    AATAAACTAGAAGTTGGATTAGGAAGAGATATATCAGGTGATCCAATTACTGTTCCACTA
    AATGAAATGCCACACTTATTGGTGGCAGGATCGACGGGTAGTGGTAAATCTGTTTGTATA
    AATGGTATTATTACAAGTATTTTATTAAATGCTAAGCCGCATGAAGTTAAACTTATGTTA
    ATCGATCCGAAAATGGTTGAACTAAATGTTTATAACGGAATTCCACATTTATTAATTCCG
    GTTGTTACAAATCCTCATAAAGCTGCTCAAGCTTTAGAAAAAATTGTAGCTGAGATGGAA
    AGACGTTATGATTTATTCCAACATTCATCAACTAGAAATATTAAAGGTTATAACGAATTA
    ATCCGTAAGCAAAATCAAGAATTAGATGAGAAGCAACCAGAATTACCTTATATCGTTGTT
    ATTGTAGATGAGCTTGCAGATTTAATGATGGTAGCTGGTAAAGAAGTTGAAAATGCGATT
    CAACGTATCACACAAATGGCACGTGCAGCAGGTATACATTTGATTGTAGCAACACAAAGA
    CCTTCTGTGGATGTAATTACAGGTATCATTAAAAATAACATTCCATCTAGAATTGCTTTT
    GCTGTGAGTTCTCAAACAGATTCAAGAACTATTATTGGTACTGGCGGTGCAGAAAAGTTA
    CTTGGTAAAGGTGACATGTTATACGTTGGAAATGGTGATTCATCACAAACACGTATTCAA
    GGGGCGTTTTTAAGTGACCAAGAGGTGCAAGATGTTGTAAATTATGTAGTAGAACAACAA
    CAGGCAAATTATGTAAAAGAAATGGAACCAGATGCACCAGTGGATAAATCGGAAATGAAA
    AGTGAAGATGCTTTATATGATGAAGCGTATTTGTTTGTTGTTGAACAACAAAAGGCAAGT
    ACATCATTGTTACAACGCCAATTTAGAATTGGTTATAATAGAGCATCTAGGTTGATGGAT
    GATTTAGAACGCAATCAGGTAATCGGTCCACAAAAAGGAAGCAAGCCTAGACAAGTTTTA
    ATAGATCTTAATAATGACGAGGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2367

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1119 [new locus tag: SA_RS06330 ]
  • symbol: SpoIIIE
  • description: hypothetical protein
  • length: 788
  • theoretical pI: 8.95758
  • theoretical MW: 88007.4
  • GRAVY: -0.283629

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype VII secretion protein EssC (TIGR03928; HMM-score: 123.9)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype VII secretion protein EccCa (TIGR03924; HMM-score: 103.1)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype VII secretion protein EccCb (TIGR03925; HMM-score: 64.9)
    Genetic information processingMobile and extrachromosomal element functionsOtherconjugative coupling factor TraD, SXT/TOL subfamily (TIGR03743; HMM-score: 15)
    type IV conjugative transfer system coupling protein TraD (TIGR02759; HMM-score: 12.3)
    MetabolismTransport and binding proteinsCations and iron carrying compoundscation transporter family protein (TIGR00860; HMM-score: 11.6)
  • TheSEED:  
    Bacterial Cell Division DNA translocase FtsK 
    Bacterial Cytoskeleton DNA translocase FtsK 
    Bacterial RNA-metabolizing Zn-dependent hydrolases DNA translocase FtsK 
  • PFAM:
    P-loop_NTPase (CL0023) FtsK_SpoIIIE; FtsK/SpoIIIE family (PF01580; HMM-score: 205.5)
    HTH (CL0123) Ftsk_gamma; Ftsk gamma domain (PF09397; HMM-score: 100.6)
    P-loop_NTPase (CL0023) AAA_10; AAA-like domain (PF12846; HMM-score: 26.4)
    AAA_19; AAA domain (PF13245; HMM-score: 20.8)
    DUF87; Domain of unknown function DUF87 (PF01935; HMM-score: 20.3)
    no clan definedFtsK_4TM; 4TM region of DNA translocase FtsK/SpoIIIE (PF13491; HMM-score: 20.1)
    P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 16.8)
    ClpP_crotonase (CL0127) Peptidase_S49_N; Peptidase family S49 N-terminal (PF08496; HMM-score: 15.9)
    P-loop_NTPase (CL0023) T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 15.5)
    TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 14.8)
    Marvel-like (CL0396) MARVEL; Membrane-associating domain (PF01284; HMM-score: 14.4)
    no clan definedNeur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region (PF02932; HMM-score: 14.4)
    CrgA; Cell division protein CrgA (PF06781; HMM-score: 14)
    P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 14)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13.4)
    no clan definedCodanin-1_C; Codanin-1 C-terminus (PF15296; HMM-score: 10.9)
    ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 6.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.252
    • Ymax_pos: 11
    • Cmax: 0.129
    • Cmax_pos: 5
    • Smax: 0.723
    • Smax_pos: 6
    • Smean: 0.628
    • D: 0.398
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAQAKKKSTAKKKTASKKRTNSRKKKNDNPIRYVIAILVVVLMVLGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQIALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLISLFGVCIITILLLCSSVILLTNHQHRDVAKVALENIKAWFGSFNEKMSERNQEKQLKREEKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQCQPSRKKRVFDAENSSNNIVNHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAKQKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLHNDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGRDISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVELNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQNQELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSVDVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQGAFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKASTSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]