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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1137 [new locus tag: SA_RS06425 ]
  • pan locus tag?: SAUPAN003600000
  • symbol: mutS
  • pan gene symbol?: mutS
  • synonym:
  • product: DNA mismatch repair protein MutS

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1137 [new locus tag: SA_RS06425 ]
  • symbol: mutS
  • product: DNA mismatch repair protein MutS
  • replicon: chromosome
  • strand: +
  • coordinates: 1291028..1293646
  • length: 2619
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTCTAATGTTACACCAATGATGCAGCAATATTTAAAAATAAAATCAGAATACCAAGAT
    TGCTTATTATTTTTTAGACTAGGTGATTTCTATGAAATGTTTTATGAAGATGCCAAGGAG
    GCATCACGTGTACTTGAAATTACTTTAACTAAAAGAGATGCTAAAAAAGAAAATCCAATT
    CCGATGTGTGGTGTTCCGTATCATTCTGCAGATAGTTATATAGATACACTTGTTAATAAT
    GGATATAAAGTAGCTATTTGTGAACAGATGGAAGATCCGAAACAAACGAAAGGTATGGTT
    AGACGTGAGGTAGTAAGAATTGTGACTCCAGGAACTGTGATGGAGCAAGGTGGTGTAGAT
    GATAAACAAAATAACTATATTTTAAGTTTTGTTATGAATCAACCTGAAATTGCGCTTAGT
    TACTGTGATGTTTCTACTGGCGAATTAAAGGTTACACATTTTAATGATGAAGCGACTTTA
    TTAAATGAAATTACGACGATAAACCCTAACGAAGTTGTTATCAATGACAATATTTCCGAT
    AATTTAAAAAGACAAATTAATATGGTGACAGAAACAATAACAGTCAGGGAAACGTTATCA
    TCAGAAATCTATAGTGTGAATCAAACTGAACATAAATTAATGTATCAAGCGACACAATTA
    TTGCTAGATTATATTCATCATACACAAAAACGTGATTTATCGCATATCGAGGATGTTGTT
    CAATATGCAGCTATAGATTATATGAAAATGGATTTTTATGCTAAGAGAAACCTTGAGTTA
    ACGGAAAGCATTCGATTAAAATCAAAAAAAGGAACGCTACTTTGGCTAATGGACGAAACG
    AAAACACCAATGGGAGCACGCCGCTTAAAACAATGGATAGATAGACCACTAATAAGTAAA
    GAACAAATTGAAGCACGATTAGATATCGTTGATGAATTTAGTGCTCATTTCATAGAAAGA
    GACACCTTAAGAACATATCTTAATCAAGTGTATGATATTGAACGTCTTGTTGGGCGTGTT
    AGTTACGGAAATGTTAATGCGAGAGATTTAATTCAACTTAAACATTCCATTTCTGAAATA
    CCGAATATTAAAGCATTACTAAATTCTATGAATCAGAATACGCTTGTACAAGTTAATCAA
    CTAGAACCCCTTGATGATTTACTTGATATATTAGAACAGAGTTTAGTAGAAGAACCACCA
    ATTTCAGTTAAAGATGGCGGACTATTCAAAGTTGGTTTTAATACGCAATTAGATGAATAT
    CTTGAAGCTTCAAAAAACGGAAAAACATGGTTAGCAGAATTACAAGCCAAAGAAAGACAA
    CGTACAGGAATAAAATCATTGAAAATAAGCTTTAATAAAGTGTTTGGTTATTTTATAGAA
    ATAACACGTGCCAACTTGCAAAATTTTGAACCAAGTGAATTTGGTTATATGAGGAAGCAA
    ACGTTATCGAATGCTGAACGTTTTATAACTGATGAACTTAAAGAAAAAGAAGATATCATT
    TTAGGTGCGGAAGACAAAGCCATCGAATTAGAATATCAATTATTTGTTCAGCTACGTGAA
    GAAGTTAAAAAATATACTGAACGTTTACAACAACAAGCTAAAATTATTTCAGAGCTAGAT
    TGTTTACAGAGCTTTGCAGAAATTGCTCAAAAATATAATTACACTAGGCCTTCATTTAGT
    GAAAATAAAACATTAGAATTAGTGGAATCTAGGCACCCAGTAGTGGAAAGAGTAATGGAT
    TATAATGACTATGTGCCTAATAATTGTCGATTAGATAATGAAACATTTATATATTTAATT
    ACAGGTCCGAATATGTCTGGTAAATCGACATATATGAGACAAGTTGCCATAATTAGTATA
    ATGGCCCAAATGGGAGCTTATGTCCCTTGTAAAGAGGCAGTGTTACCTATATTTGATCAA
    ATATTCACTAGAATAGGTGCGGCAGATGATTTGGTTTCAGGTAAGAGTACGTTTATGGTA
    GAAATGCTAGAAGCACAAAAGGCATTAACTTATGCAACAGAGGATAGTTTGATTATTTTC
    GATGAAATTGGACGTGGTACTTCAACGTATGACGGTTTAGCTTTAGCGCAGGCAATGATA
    GAGTATGTAGCTGAAACATCGCATGCTAAAACGTTATTTTCAACACATTATCATGAATTG
    ACAACATTAGATCAAGCATTACCAAGTCTAAAAAATGTTCACGTCGCTGCTAATGAATAT
    AAAGGTGAACTTATATTCTTGCATAAAGTCAAAGATGGTGCAGTTGACGATAGTTATGGT
    ATTCAAGTTGCGAAATTAGCTGATTTACCTGAAAAAGTTATTAGCAGAGCACAAGTGATC
    CTAAGCGAGTTTGAAGCGTCTGCTGGTAAAAAATCATCGATATCAAATTTAAAAATGGTC
    GAAAATGAACCTGAAATTAATCAAGAAAATTTAAACTTAAGTGTTGAAGAAACAACTGAT
    ACTTTATCTCAAAAAGACTTTGAACAAGCATCATTTGATTTGTTTGAAAATGATCAAGAA
    AGCGAGATTGAACTACAAATTAAAAATTTGAATTTATCTAATATGACACCAATTGAGGCA
    TTGGTGAAGTTAAGTGAATTACAAAATCAATTAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2619

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1137 [new locus tag: SA_RS06425 ]
  • symbol: MutS
  • description: DNA mismatch repair protein MutS
  • length: 872
  • theoretical pI: 4.62947
  • theoretical MW: 99903
  • GRAVY: -0.376032

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA mismatch repair protein MutS (TIGR01070; HMM-score: 1090.7)
    Genetic information processingDNA metabolismOtherMutS2 family protein (TIGR01069; HMM-score: 147.8)
  • TheSEED:  
    DNA MetabolismDNA repairDNA repair, bacterial MutL-MutS system DNA mismatch repair protein MutS 
  • PFAM:
    P-loop_NTPase (CL0023) MutS_V; MutS domain V (PF00488; HMM-score: 275.6)
    no clan definedMutS_I; MutS domain I (PF01624; HMM-score: 164.3)
    MutS_III; MutS domain III (PF05192; HMM-score: 151.2)
    MutS_IV; MutS family domain IV (PF05190; HMM-score: 96.5)
    MutS_II; MutS domain II (PF05188; HMM-score: 65.1)
    P-loop_NTPase (CL0023) AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 17.4)
    AAA_14; AAA domain (PF13173; HMM-score: 16.6)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 11.3)
    AAA_23; AAA domain (PF13476; HMM-score: 9.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.124
    • Ymax_pos: 15
    • Cmax: 0.119
    • Cmax_pos: 39
    • Smax: 0.188
    • Smax_pos: 11
    • Smean: 0.15
    • D: 0.134
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRVLEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRREVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATLLNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQATQLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWLMDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIERLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILEQSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKISFNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAIELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLELVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQMGAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDEIGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEYKGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLKMVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMTPIEALVKLSELQNQLK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]