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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1147 [new locus tag: SA_RS06475 ]
  • pan locus tag?: SAUPAN003623000
  • symbol: SA1147
  • pan gene symbol?: hflX
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1147 [new locus tag: SA_RS06475 ]
  • symbol: SA1147
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1304040..1305278
  • length: 1239
  • essential: yes DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCTCAGCAACAAATTCATGATACTAAAAATAAACTAGAAAAAGCTGTCTTAGTCGGT
    GTACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCT
    TTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTA
    GATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTC
    AAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTA
    AATGAAGCTTTAGGTGTAAAAATTATTGATAGAACTCAGTTGATTCTTGAAATATTTGCA
    TTAAGAGCAAGAAGTAAAGAAGGTAAATTGCAAGTAGAGCTAGCACAACTTGATTATTTA
    TTACCTAGATTGCAAGGCCATGGTAAAAGTCTTTCTCGTTTAGGTGGCGGTATTGGAACT
    AGAGGCCCTGGTGAAACGAAGTTAGAGATGGATCGCAGACATATTCGAACTCGTATGAAT
    GAAATTAAACATCAATTGCGGACGGTAGAAGAACATCGCGAAAGATATCGAAATAAAAGA
    AATCAAAATCAGGTGTTTCAAGTAGCTTTAGTTGGTTATACAAATGCTGGTAAATCATCA
    TGGTTTAATGTTTTAGCAAATGAAGAGACGTATGAAAAAGATCAATTATTTGCAACGTTA
    GATCCTAAAACACGACAAATTCAAATAAATGATGGATTTAATTTAATTATTTCAGATACT
    GTTGGTTTTATACAGAAACTACCTACGACGTTAATTGCAGCTTTTAAATCAACTTTAGAA
    GAGGCTAAAGGTGCAGATTTATTAGTACATGTCGTAGATAGTAGCCATCCTGAATACCGT
    ACGCAGTATGACACAGTTAATGATTTAATCAAACAATTAGATATGAGTCATATTTCTCAA
    ATAGTTATTTTTAATAAAAAGGACTTATGTGATCATGCATCAAATCGTCCAGCAAGTGAT
    TTGCCTAATGTTTTTGTTTCTTCTAAAAATGATGGTGATAAATTACTTGTTAAGACGTTA
    TTTATTGATGAAATCAAAAGGCAATTAACTTATTATGATGAGACAATTGCGACGAATAAT
    GCAGATCGATTATATTTTCTAAAACAACATACATTAGTGACTGAACTTAAATATGATGAA
    ATTGAAAATGTTTATCGTATAAAAGGATTTAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1239

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1147 [new locus tag: SA_RS06475 ]
  • symbol: SA1147
  • description: hypothetical protein
  • length: 412
  • theoretical pI: 7.1298
  • theoretical MW: 47335.4
  • GRAVY: -0.551214

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionGeneralGTP-binding protein HflX (TIGR03156; HMM-score: 466.4)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 56.6)
    Genetic information processingProtein synthesisOtherribosome-associated GTPase EngA (TIGR03594; HMM-score: 45.5)
    Genetic information processingProtein synthesisOtherObg family GTPase CgtA (TIGR02729; HMM-score: 42.6)
    Genetic information processingProtein fateProtein modification and repair[FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 40.4)
    Genetic information processingProtein synthesisOtherribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 35.7)
    Unknown functionGeneralsmall GTP-binding protein domain (TIGR00231; HMM-score: 32.1)
    Genetic information processingProtein synthesisOtherGTP-binding protein Era (TIGR00436; HMM-score: 29.3)
    Genetic information processingProtein synthesisOtherribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 27.4)
    Genetic information processingProtein synthesisOtherribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 20.1)
    Unknown functionGeneralGTP-binding protein YchF (TIGR00092; HMM-score: 15.5)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsferrous iron transport protein B (TIGR00437; HMM-score: 10.8)
  • TheSEED:  
    Hfl operon Ribosome LSU-associated GTP-binding protein HflX 
    Universal GTPases Ribosome LSU-associated GTP-binding protein HflX 
  • PFAM:
    no clan definedGTP-bdg_M; GTP-binding GTPase Middle Region (PF16360; HMM-score: 110.3)
    GTP-bdg_N; GTP-binding GTPase N-terminal (PF13167; HMM-score: 108.2)
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 66.7)
    Arf; ADP-ribosylation factor family (PF00025; HMM-score: 23.4)
    cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 22.2)
    Gtr1_RagA; Gtr1/RagA G protein conserved region (PF04670; HMM-score: 22.1)
    FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 21.7)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 19.2)
    Thioredoxin (CL0172) DUF4174; Domain of unknown function (DUF4174) (PF13778; HMM-score: 16.2)
    P-loop_NTPase (CL0023) SRPRB; Signal recognition particle receptor beta subunit (PF09439; HMM-score: 15.7)
    ABC_tran; ABC transporter (PF00005; HMM-score: 14.7)
    Dynamin_N; Dynamin family (PF00350; HMM-score: 13.8)
    Chelatase (CL0043) ZnuA; Zinc-uptake complex component A periplasmic (PF01297; HMM-score: 13)
    no clan definedMMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.148
    • Ymax_pos: 11
    • Cmax: 0.128
    • Cmax_pos: 24
    • Smax: 0.321
    • Smax_pos: 5
    • Smean: 0.224
    • D: 0.177
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSETCQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNEALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGTRGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGKSSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFKSTLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHASNRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTLVTELKYDEIENVYRIKGFKK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]