From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1163 [new locus tag: SA_RS06605 ]
  • pan locus tag?: SAUPAN003698000
  • symbol: SA1163
  • pan gene symbol?: thrD
  • synonym:
  • product: aspartate kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1163 [new locus tag: SA_RS06605 ]
  • symbol: SA1163
  • product: aspartate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1323919..1325301
  • length: 1383
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAAGTTTCCAAATTCGGTGGTAGCTCCGTCTCCAATGCTACTCAAATTAAAAAGGTT
    TTAAATATTGTTAATTCTGATCCAGAGCGAAAGATTGTTATCGTTTCTGCTCCAGGTAAA
    AGACATGATAATGATATTAAAACAACTGATTTGTTAATCAGATTATATGAAAAGGTCATT
    AATCATCTTGATTATCATGATAAAAAAAGAGAAATTATTCAGCGTTATGATGATATTGTA
    AAAGAATTGCAAATGGATGAAAGTATTTTACGGACGATAGATGTGACTTTGGAACATTAT
    ATTAATCAATTAAAAAATGAACCAAAGAGACTATTAGATGCATTACTTTCTTGTGGTGAA
    GATTTTAATGCGCAATTGATAGCTTTATATAATAATAGTCAAGGTATACCAACAAAATAT
    ATATCACCGAAGGAAGCGGGTATTTTTGTTACTGACGTCCCACAAGAAGCACAAATATTA
    GATAGTGCTTACTCTGAAATTAAAAAATTAAGAGATTATGATGAAAAACTAATTATCCCA
    GGATTTTTTGGCGTATCTCATGAAGGATATATAGTTACATTTCCACGTGGCGGATCAGAT
    ATAACTGGCGCCATCATATCAAGTGGTGTTAGAGCCACAATTTATGAAAATTTCACCGAT
    GTGTCTGGTATTTATAAAGCTAATCCAAATATCATCAAAGATCCTGAATTAATCGAAGAA
    ATCACTTATCGAGAAATGCGTGAATTATCTTATGCTGGTTTTAGTGTTTTCCATGATGAA
    GCCTTACAACCACTTTATAAAGATCGCATCCCTGTGGTGATTAAAAATACGAACCGCCCT
    CAAGATAAGGGTACATTTATAGTGCATGATAGAGAAATAAATGCGAAAAATGTGATTAGC
    GGTATAAGTTGTGATAAAGATTTTACTGTCATTAATATCAAAAAGTATTTAATGAATAGA
    CAAGTCGGCTTTACTAGAAAGATATTAGGTGTCTTAGAGGATAATAATATTTCATTTGAT
    CATATGCCTTCAGGTATTGATACTATCAGTATCGTTATGCGTTCAAAACAAATTCAAAAT
    AAAGAAACAAAAGTATTAAATGAAATTCGTCAAAAATGTGATGTTGATGAGCTAAGTATT
    GAGCATGATTTAGCAATATTAATGATTGTCGGCGAAGGTATGAATAAAGTCATCGGTACT
    GCAAATAAAATTACACATGCCTTAGCTGAATCAAAAATTAACTTAAAAATGATTAACCAA
    GGTGCTTCGGAAATCTCGATGATGTTTGGAATTCATGTAGATGATGCTGAAAAAGCTGTA
    TTATCTACATATGAGTTTTGCTATCACGGGGTTGCATTAAAAAACTTGTGTGATAAAAAC
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1383

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1163 [new locus tag: SA_RS06605 ]
  • symbol: SA1163
  • description: aspartate kinase
  • length: 460
  • theoretical pI: 6.38678
  • theoretical MW: 52003.3
  • GRAVY: -0.28087

Function[edit source | edit]

  • reaction:
    EC 2.7.2.4?  ExPASy
    Aspartate kinaseATP + L-aspartate = ADP + 4-phospho-L-aspartate
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAspartate familyaspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 285.2)
    MetabolismAmino acid biosynthesisAspartate familyaspartate kinase, monofunctional class (TIGR00656; EC 2.7.2.4; HMM-score: 254.9)
    MetabolismAmino acid biosynthesisAspartate familyputative Pyrococcus aspartate kinase subunit (TIGR02078; EC 2.7.2.4; HMM-score: 91.3)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionsputative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 20.5)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineLysine Biosynthesis DAP Pathway Aspartokinase (EC 2.7.2.4) 
    Lysine, threonine, methionine, and cysteineThreonine and Homoserine Biosynthesis Aspartokinase (EC 2.7.2.4) 
  • PFAM:
    no clan definedAA_kinase; Amino acid kinase family (PF00696; HMM-score: 142.4)
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 36.2)
    ACT_7; ACT domain (PF13840; HMM-score: 31.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.176
    • Ymax_pos: 11
    • Cmax: 0.121
    • Cmax_pos: 27
    • Smax: 0.422
    • Smax_pos: 1
    • Smean: 0.281
    • D: 0.217
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA1163 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]