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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1256 [new locus tag: SA_RS07125 ]
  • pan locus tag?: SAUPAN003854000
  • symbol: SA1256
  • pan gene symbol?: msrB
  • synonym:
  • product: methionine sulfoxide reductase B

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1256 [new locus tag: SA_RS07125 ]
  • symbol: SA1256
  • product: methionine sulfoxide reductase B
  • replicon: chromosome
  • strand: -
  • coordinates: 1430012..1430440
  • length: 429
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGCTTAAAAAAGATAAAAGTGAACTAACAGACATAGAATATATTGTTACACAAGAAAAT
    GGCACTGAACCACCATTTATGAATGAATATTGGAATCATTTTGCTAAAGGGATTTATGTA
    GATAAGATTTCTGGTAAACCTTTATTTACATCTGAAGAAAAGTTTCATTCTGAATGTGGA
    TGGCCTAGCTTTTCTAAAGCGCTTGATGACGATGAAATTATAGAATTAGTCGACAAATCA
    TTTGGTATGGTAAGAACTGAAGTGCGTTCAGAAGAATCAAATAGTCATTTAGGACACGTC
    TTTAATGATGGACCTAAAGAAAGTGGCGGCTTAAGATACTGTATCAATTCCGCTGCAATT
    CAATTTATTCCATATGAAAAATTAGAAGAATTGGGTTATGGCGATTTAATATCACATTTT
    GATAAGTAG
    60
    120
    180
    240
    300
    360
    420
    429

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1256 [new locus tag: SA_RS07125 ]
  • symbol: SA1256
  • description: methionine sulfoxide reductase B
  • length: 142
  • theoretical pI: 4.49255
  • theoretical MW: 16263
  • GRAVY: -0.598592

Function[edit source | edit]

  • reaction:
    EC 1.8.4.12?  ExPASy
    Peptide-methionine (R)-S-oxide reductasePeptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin
    EC 1.8.4.11?  ExPASy
    Peptide-methionine (S)-S-oxide reductasePeptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxinL-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin
  • TIGRFAM:
    Cellular processesCellular processesAdaptations to atypical conditionsmethionine-R-sulfoxide reductase (TIGR00357; EC 1.8.4.-; HMM-score: 192.3)
    Genetic information processingProtein fateProtein modification and repairmethionine-R-sulfoxide reductase (TIGR00357; EC 1.8.4.-; HMM-score: 192.3)
  • TheSEED:  
    Peptide methionine sulfoxide reductase Peptide-methionine (R)-S-oxide reductase MsrB (EC 1.8.4.12) 
  • PFAM:
    Beta-tent (CL0080) SelR; SelR domain (PF01641; HMM-score: 159.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.105
    • Ymax_pos: 30
    • Cmax: 0.108
    • Cmax_pos: 36
    • Smax: 0.144
    • Smax_pos: 2
    • Smean: 0.094
    • D: 0.101
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKISGKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMVRTEVRSEESNSHLGHVFNDGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J. Microbiol. Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)
Grazyna Młynarczyk, Andrzej Młynarczyk, Ksenia Szymanek, Mirosław Luczak
[The frequency of the occurrence of genes ermA, ermB, ermC and msrA/B among methicillin-resistant Staphylococcus aureus strains resistant to erythromycin]. [Czestość wystepowania genów ermA, ermB, ermC i msrA/B u metycylino-opornych klinicznych szczepów Staphylococcus aureus opornych na erytromycyne izolowanych w Polsce.]
Med Dosw Mikrobiol: 2006, 58(3);183-90
[PubMed:17340992] [WorldCat.org] (P p)