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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1274 [new locus tag: SA_RS07220 ]
  • pan locus tag?: SAUPAN003895000
  • symbol: SA1274
  • pan gene symbol?: dynA
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA1274 [new locus tag: SA_RS07220 ]
  • symbol: SA1274
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1476727..1480167
  • length: 3441
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATTAATAAAGAACAATTAGATCTTTTATATAAATTAAAAAAAGAAGTTGAAAAGTCG
    CGAAATGAAGCACTTTTACATACAATTAACCAAGTAATTAAGAAAGTATATTTGCAGCAA
    TATACATGTTCGTTCGTTGGACATTTTTCTGCAGGTAAATCGACACTGATAAATTTATTA
    ATTGAACAAGATATCTTACCAAGTTCACCTGTACCAACGACAAGTAATACTGCTATTGTG
    TCAGTTTCAGACAATCACGATATTATTGCTAATTTGCCGAATCAAACATATGCCAAATTA
    TCTAATTATGATGAAGTAAGGGAAATGAATCGCCAAAATGTCGACGTTGAATCTGTAGAA
    ATTAATTTTCAATCAGCTAAATTTGAAAATGGGTTTACGTTGCAAGATACACCAGGTGTT
    GATTCAAATGTTGCATCACATCAGTCAATAACAGAACAATATATGTATACAAGTAATATG
    ATATTTTATACGGTTGACTATAACCACGTTCAATCTGAACTTAACTTTAAGTTTATGAAG
    CATATAAATGATGTTGGAATACCTGTTGTGTTTATCATTAATCAAATTGACAAGCATCAA
    GACGATGAATTGTCATTCTCTACGTTTAAATCACGAGTTGAAAAATCAATTGCAGATTGG
    GGCATTAAATTAGAACGCACCTTTTATGTATCTAAATTTGATCACCCTGAAAATGAACTT
    GAAGTATTATCAAGTTATCTAATTTCATTAGATCAACATAGAGAGACAATAGAGGATTAT
    ACATCAAGAACGGTTGAATACATTACCGAAGCTCAGCTAGATTACATTCAGTCTGAAATT
    CAGGAAGTACTAGAAGATTTAGGTATCGAAGAAGCGGAGTTTGAACAAGCATTTTTAAAT
    AGTCAACAACATCAAGCAATTAGTGAAGAGGCACAACTTTTAAATAATCCAGATGAATTA
    ATGGCATTTTTAAAGAATAAGCGTAAAAATATTTTAGAAAATGCATACATTATGCCGCAT
    AATATGAGAGAAATGTTACGAAGTTATTTGGAAAGTATGTCTCAAGATTTTAATGTTGGT
    GGGTTTTTTAATAAAAAGAAGAAAAAGCTACAAATTCAACAACAGCGATTATTAACAGCG
    ACAGATGCGTTACAAGAACATGTTAATCAACAAATTCGTCAACCAATGCGAGAAGATATG
    TCATTTGTAACGCGTTTTATCAATAAAAAAGAAGCTTCAGATAAAGTATTAAATCAGCAT
    TATGACGTTAAACCGGAAATGATTGAAGATTTATATCAACCACAAACATCAATCAGCAAT
    ACTTATGTACTTACATTTTCAGACGAAGTGGTTAAAGCCATTAAGAAATATGTTGAACAA
    CAATCAACACCGATTTTTAAAGAAATAATAGAAAATGTGCAGGCAGATGAATTACCAACA
    GAAGAAAGTGATGATTTAAAAGAATATCAACGTTATACAGAATTAAATGAGCTGCGTCAG
    TCATTGACGACTAAGAATTATCGTCACTACTATATTCATTTAGATGAATCTCTAGATAAA
    TTAATAGGTCGACAAGAGACAACATATCAAGTGGCTACTGATAATACTCAGGATAATCGT
    GATAATCAGCAGCTAAATCAAAATACAGCTACAACAAATATGTCTATAGATATTCAAAAA
    GCGCTTGATATAATTTCGGATGTGCCTTTGTTCAAGCGTACAAAGCAAGATATCCACGAA
    ACATTAACACGTATAGATAATAAATTAATAAAAATAGGTGTATTTGGAACATTTAGTGCG
    GGTAAAAGTAGTTTGATAAATGCATTATTAGGCGAGCATATTTTAGTGAGTTCTCCAAAT
    CCTACTACAGCAGCTACTACAGAAATATCTTATGGAGACGAGAGTTATATAACGCTAAAA
    TCACAATCGCAATTATTAGATGAAATTAATGCAGTAGTTGAATACCAAGATATATCTTTT
    TCAACTATAGAAGAATTTATTAATTGGGATTTAGAAAAGTTAAAATCACATTTAAATAAA
    AATCAACTCGCTTTTATTCAGGCAGTTGAAAAACATTATAAATTGTATGTCAATATGTTG
    GAAAATGGAGAAAAACATGCCATTAATCAACAGGAATTGAAAAAGTGGAGTGCAGAAGAT
    GAATATGCAACATTTGTTAAGACAGTACACATTGCATTAATGCATGATTGGTTAAAAGGT
    AAAATAATTGTTGATTCATTAGGGCTACACTCAAATAACCAAAGGCATACAAATGAAACC
    GAACAAATTTTAACTTCTTCAGACTTAATATTGTATGTAAGTTATTTTAATCATTCATTT
    ACTGATAATGACAAAGCGTTTATAGAACACATGAAAGATATGAACCAGTTGAATGAAAAC
    CAAGCATTTAAAATGGTAATTAATGCTGCTGATTTAGCAGAAAGTCAAGATGATCTTGAA
    GCAGTTGAAACATATGTATCAGATGCATTAAGACAAGTACACTTACAATCAGACATTTTT
    GCTGTATCAAGTCGAAATGCATTGCAAGCTGAAGATAAGGGCATTGATCAATTAAAACAA
    AGCATACAACAATTTGTTGATGTTGAATCTAAATCAATTTTAGAACAACAAATGATTCAT
    CAGCTTCAACAAATGGATCGTTCTTATGTAGAGATGATTACAGAATTTGAAACAAATAAA
    GCTGATATTTCACGTCGCCAACAAAGGTTAACAGATTATAAAGATAAACAGCGTTTACAA
    CATCAATTAATTGATGCGACGCTACAACATACAGACAACGAAGTTGAAGAACAAGTTTAT
    CATTTAAATGCACGTTTAAAATTACAACTACTTGACGATGTTAAATCAGTGTTTAATTCT
    CAAATGACGCAAAATAGTGATTTTAATGAAGAAAAGAAAGTGTCTACGAAAGTATACTTA
    GATCAAATTCATCAACGATTGTTTTTAGAGCAATCTTTAATTACAGAACGTATAAAAAAA
    TACTTTAATAAGCAACTCACTAAGCAAATTGCACCAATCGTTCAACAATTAGCAGATTTA
    CATGTCATTGTTAATCCTCAGTTTAACTTTGAATCAGCTAATATAGAGCAACCATTATTG
    CACATCGATTTCAATGATATGCTAAATGCATTGCCTAAACAATTAACAAAACGTAAAATT
    TTGAATCCAAATGGGCAAAGAGATATACATGAATCAATTTGTCAAAGTACGTTAGGATTA
    TTACAACCACAAATGGGATTATTGAGGCAACAGCTTGAATTATATGTAAAGCAAATGGCT
    GTAGAAGCTGAATCGCAATTTGAAAGTTTTGAAGCTAATATTCAAACGCAAATAAACGAT
    TTATTAGCATTTGATTTAGATACAACACTTATCAATCAATTGAAAGATAAACATCAACAA
    CTGAAAACTATTTTATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3441

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA1274 [new locus tag: SA_RS07220 ]
  • symbol: SA1274
  • description: hypothetical protein
  • length: 1146
  • theoretical pI: 4.83651
  • theoretical MW: 133231
  • GRAVY: -0.567277

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 66.7)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 60.6)
    and 4 more
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 49)
    Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 30.7)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 28.8)
    Metabolism Energy metabolism Amino acids and amines ethanolamine utilization protein, EutP (TIGR02528; HMM-score: 19)
  • TheSEED  :
    • Uncharacterized protein Bsu YpbR
  • PFAM:
    P-loop_NTPase (CL0023) Dynamin_N; Dynamin family (PF00350; HMM-score: 207)
    and 9 more
    MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 63.7)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 29.2)
    FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 26.3)
    GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 20)
    ArgK; ArgK protein (PF03308; HMM-score: 14.8)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13.5)
    no clan defined E1_FCCH; Ubiquitin-activating enzyme E1 FCCH domain (PF16190; HMM-score: 12.9)
    P-loop_NTPase (CL0023) AAA_28; AAA domain (PF13521; HMM-score: 10.1)
    AAA_18; AAA domain (PF13238; HMM-score: 9.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012476
    • TAT(Tat/SPI): 0.001
    • LIPO(Sec/SPII): 0.001018
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCSFVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSNYDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNMIFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIADWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYIQSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILENAYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQIRQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEDLYQPQTSISNTYVLTFSDEVVKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYRHYYIHLDESLDKLIGRQETTYQVATDNTQDNRDNQQLNQNTATTNMSIDIQKALDIISDVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTAATTEISYGDESYITLKSQSQLLDEINAVVEYQDISFSTIEEFINWDLEKLKSHLNKNQLAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKGKIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLNENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGIDQLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYKDKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEKKVSTKVYLDQIHQRLFLEQSLITERIKKYFNKQLTKQIAPIVQQLADLHVIVNPQFNFESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLLRQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]