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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1310 [new locus tag: SA_RS07425 ]
  • pan locus tag?: SAUPAN003943000
  • symbol: ansA
  • pan gene symbol?: ansA
  • synonym:
  • product: L-asparaginase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1310 [new locus tag: SA_RS07425 ]
  • symbol: ansA
  • product: L-asparaginase
  • replicon: chromosome
  • strand: +
  • coordinates: 1516323..1517291
  • length: 969
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAACATCTACTTGTTATTCATACTGGTGGCACCATTAGTATGTCACAAGACCAATCT
    AATAAAGTAGTAACAAATGATATCAACCCTATTTCAATGCATCAAGATGTCATAAATCAA
    TATGCACAAATAGATGAATTAAATCCTTTTAATGTACCATCACCTCATATGACAATCCAA
    CATGTTAAACAATTAAAGGATATTATTTTAGAAGCAGTAACAAATAAATATTATGATGGT
    TTCGTTATCACGCATGGTACCGATACGTTAGAAGAAACTGCCTTTTTACTTGATTTAATA
    TTAGGTATCGAGCAACCTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGT
    TCTGACGGATTATATAATTATATTTCCGCTATTCGCGTTGCCTCTGATGAAAAGGCCCGT
    CATAAAGGCGTGATGGTTGTATTTAATGATGAAATTCATACGGCGCGTAATGTTACCAAA
    ACACATACGTCTAATACAAACACATTTCAAAGTCCAAATCATGGTCCGCTAGGTGTATTG
    ACAAAGGATCGTGTGCAATTCCATCATATGCCATATCGCCAACAAGCATTGGAAAATGTC
    AATGACAAACTAAATGTACCATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTT
    AGTTTTTATAGTCGTGAAGGTATCGATGGTATGGTTATTGAAGCGTTAGGACAAGGCAAC
    ATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTAAATATACCTATTGTG
    CTAGTTTCACGTTCCTTTAATGGTATTGTGAGTCCAACTTACGCATACGATGGTGGTGGT
    TACCAACTCGCACAACAAGGTTTTATTTTTTCTAACGGTTCGAATGGTCCAAAAGCAAGA
    TTAAAATTATTAGTTGCGTTAAGCAACAATTTAGATAAAGCTGAAATCAAATCATATTTT
    GAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1310 [new locus tag: SA_RS07425 ]
  • symbol: AnsA
  • description: L-asparaginase
  • length: 322
  • theoretical pI: 6.3723
  • theoretical MW: 35552.2
  • GRAVY: -0.165217

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismEnergy metabolismAmino acids and aminesL-asparaginase, type II (TIGR00520; EC 3.5.1.1; HMM-score: 230.6)
    L-asparaginase, type I (TIGR00519; EC 3.5.1.1; HMM-score: 174.6)
    Genetic information processingProtein synthesistRNA aminoacylationglutamyl-tRNA(Gln) amidotransferase, subunit D (TIGR02153; EC 6.3.5.-; HMM-score: 130.9)
  • TheSEED:  
    Amino Acids and DerivativesGlutamine, glutamate, aspartate, asparagine; ammonia assimilationGlutamine, Glutamate, Aspartate and Asparagine Biosynthesis L-asparaginase (EC 3.5.1.1) 
  • PFAM:
    no clan definedAsparaginase; Asparaginase, N-terminal (PF00710; HMM-score: 201)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.217
    • Ymax_pos: 23
    • Cmax: 0.159
    • Cmax_pos: 23
    • Smax: 0.421
    • Smax_pos: 16
    • Smean: 0.265
    • D: 0.235
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 15927060 NCBI
  • RefSeq: NP_374593 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTKTHTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGSNGPKARLKLLVALSNNLDKAEIKSYFEL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • ansA no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]