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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1394 [new locus tag: SA_RS07880 ]
  • pan locus tag?: SAUPAN004146000
  • symbol: glyS
  • pan gene symbol?: glyS
  • synonym:
  • product: glycyl-tRNA synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1394 [new locus tag: SA_RS07880 ]
  • symbol: glyS
  • product: glycyl-tRNA synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 1600376..1601767
  • length: 1392
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAAAAGATATGGATACAATTGTTTCATTAGCAAAACACAGAGGTTTTGTGTTCCCT
    GGTAGTGATATTTACGGTGGTTTATCAAACACATGGGATTATGGTCCTTTAGGTGTTGAA
    TTAAAGAATAATGTTAAAAAAGCTTGGTGGCAAAAATTCATTACACAATCACCGTTTAAC
    GTTGGTATCGACGCTGCAATCTTAATGAATCCAAAAGTATGGGAAGCTTCAGGACACTTA
    AACAACTTCAACGACCCAATGATTGATAATAAAGATAGTAAAATTCGATATCGCGCTGAT
    AAATTAATTGAAGATTATATGCAAGATGTTAAAGGTGATGAAAACTTCATTGCCGATGGT
    TTAAGTTTTGAACAAATGAAAAAAATTATTGACGATGAAGGTATTGTTTGTCCTGTAAGT
    AAAACTGCTAACTGGACTGAAATTCGCCAATTCAATTTAATGTTTAAAACATTCCAAGGT
    GTAACTGAAGATTCTACAAATGAAATTTTCTTACGTCCTGAAACAGCACAAGGTATTTTT
    GTAAACTATAAAAACGTGCAACGTTCAATGCGTAAAAAATTACCATTTGGTATCGGTCAA
    ATTGGTAAATCATTCCGTAATGAAATCACTCCAGGTAACTTCATTTTCAGAACAAGAGAA
    TTTGAACAAATGGAACTTGAATTCTTCTGTAAACCAGGAGAAGAAATCGAATGGCAAAAT
    TATTGGAAAACTTTTGCAAGTGACTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATG
    CGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATT
    GAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTC
    GACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACG
    AACGAAAAATATATTCCATATTGTATCGAGCCATCACTTGGTGCAGATCGTGTAACATTA
    GCTTTCTTATGTGATGCATATGATGAAGAAGGCGTTGAAGGTAGTAAAGATGCACGTACA
    GTTTTACACTTCCATCCTGCATTAGCACCATATAAAGCAGCGATTTTACCTTTAAGTAAG
    AAATTATCTGGCGAAGCGATTAAGATTTTTGAGCAATTAAGTTCTAAATTCTCAATCGAT
    TTCGATGAATCACAATCTATCGGTAAAAGATACCGTCGTCAAGATGAAATCGGTACACCT
    TATTGTGTAACATTCGACTTTGATTCATTAGAAGATAATCAAGTTACAGTACGTGACAGA
    GATTCAATGGAACAAGTTCGTATGCCAATCTCAGAGTTAGAAGCTTTCTTAACTGAAAAA
    ACAAAATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1392

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1394 [new locus tag: SA_RS07880 ]
  • symbol: GlyS
  • description: glycyl-tRNA synthetase
  • length: 463
  • theoretical pI: 4.7417
  • theoretical MW: 53619.9
  • GRAVY: -0.588769

Function[edit source | edit]

  • reaction:
    EC 6.1.1.14?  ExPASy
    Glycine--tRNA ligaseATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationglycine--tRNA ligase (TIGR00389; EC 6.1.1.14; HMM-score: 525.6)
    Genetic information processingProtein synthesistRNA aminoacylationthreonine--tRNA ligase (TIGR00418; EC 6.1.1.3; HMM-score: 54.8)
    Genetic information processingProtein synthesistRNA aminoacylationproline--tRNA ligase (TIGR00409; EC 6.1.1.15; HMM-score: 38.8)
    Genetic information processingProtein synthesistRNA aminoacylationhistidine--tRNA ligase (TIGR00442; EC 6.1.1.21; HMM-score: 37.7)
    Genetic information processingProtein synthesistRNA aminoacylationproline--tRNA ligase (TIGR00408; EC 6.1.1.15; HMM-score: 28.5)
    Genetic information processingProtein synthesistRNA aminoacylationserine--tRNA ligase (TIGR00414; EC 6.1.1.11; HMM-score: 15.9)
  • TheSEED:  
    Glycyl-tRNA synthetase (EC 6.1.1.14) 
  • PFAM:
    IIaaRS-ABD (CL0458) HGTP_anticodon; Anticodon binding domain (PF03129; HMM-score: 84.1)
    tRNA_synt_II (CL0040) tRNA-synt_2b; tRNA synthetase class II core domain (G, H, P, S and T) (PF00587; HMM-score: 37.9)
    no clan definedPHF12_MRG_bd; PHD finger protein 12 MRG binding domain (PF16737; HMM-score: 12.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA2041(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SA0627hypothetical protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.133
    • Ymax_pos: 11
    • Cmax: 0.108
    • Cmax_pos: 42
    • Smax: 0.242
    • Smax_pos: 4
    • Smean: 0.178
    • D: 0.15
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNNVKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKLIEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQGVTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRTREFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNATTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLGADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQLSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPISELEAFLTEKTKF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • glyS no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator: T-box(Gly)
    T-box(Gly) (RNA) important in Amino acid metabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J. Microbiol. Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)