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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1415 [new locus tag: SA_RS07990 ]
  • pan locus tag?: SAUPAN004173000
  • symbol: holA
  • pan gene symbol?: holA
  • synonym:
  • product: DNA polymerase III subunit delta

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1415 [new locus tag: SA_RS07990 ]
  • symbol: holA
  • product: DNA polymerase III subunit delta
  • replicon: chromosome
  • strand: -
  • coordinates: 1621306..1622280
  • length: 975
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGCGACAATATTGTAGCTATTTATGGAGATGTGCCTGAATTGGTTGAAAAACAAAGT
    GCAGAAATCATATCACAATTTTTGAAAAGTGATAGAGATGACTTTAACTTTGTGAAATAT
    AATTTATACGAAAAAGAGATTGCACCAATTGTTGAAGAAACATTAACATTGCCTTTCTTT
    TCAGATAAAAAAGCAATTTTGGTTAAAAATGCATATATATTTACAGGTGAAAAAGCGCCA
    AAAGATATGGCTCATAATGTAGATCAATTAATAGAATTTATTGAAAAATATGATGGCGAA
    AATTTGATTGTCTTTGAGATATATCAAAATAAACTTGATGAAAGAAAAAAGTTAACTAAA
    ACTCTAAAAAAGCATGCAAGGCTTAAAAAAATAGAGCAAATGTCTGAAGAAGAAATAAAA
    AAATGGATTCAAAGTAAATTAAATGAGAATTTCAAAGATATCAAAAGAGATGCATTAGAT
    TTATTTATTGAGTTGACAGGTATTAACTTTAATATTGTCTCACAAGAGATAGAAAAGTTG
    ATTTTATTTTTAGGCGATAGACCAACAATTAATAAGCAGGATGTTAACCAAATTATTAAT
    AGAAGTTTAGAGCAAAATGTATTTTTACTGACTGAATACATTCAGAAAAGAAAGAAAGAA
    CAAGCAATTCATTTAGTAAAAGATTTAATAACTATGAAAGAAGAACCAATTAAATTACTT
    GCACTAATTACAAGTAATTACCGATTATTTTATCAATGTAAGATTCTGAGTCAAAAAGGA
    TATAGTGGACAGCAAATTGCTAAAACAATAGGAGTGCATCCATACAGAGTAAAATTAGCG
    TTAGGACAAGTAAGACATTATCAACTTGATGAATTATTGAATATTGTAGATGCTTGTGCG
    GAAACTGATTATAAACTTAAATCATCATATATGGATAAACAGTTAATACTGGAATTATTC
    ATTCTATCTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    975

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1415 [new locus tag: SA_RS07990 ]
  • symbol: HolA
  • description: DNA polymerase III subunit delta
  • length: 324
  • theoretical pI: 7.06335
  • theoretical MW: 37948.8
  • GRAVY: -0.311111

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase III, delta subunit (TIGR01128; EC 2.7.7.7; HMM-score: 296.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase III, subunit gamma and tau (TIGR02397; EC 2.7.7.7; HMM-score: 14.9)
  • TheSEED:  
    DNA MetabolismDNA replicationDNA-replication DNA polymerase III delta subunit (EC 2.7.7.7) 
  • PFAM:
    P-loop_NTPase (CL0023) DNA_pol3_delta; DNA polymerase III, delta subunit (PF06144; HMM-score: 170.2)
    no clan definedDUF2018; Domain of unknown function (DUF2018) (PF09442; HMM-score: 17.2)
    DUF3781; Protein of unknown function (DUF3781) (PF12636; HMM-score: 15.6)
    DisA-linker; DisA bacterial checkpoint controller linker region (PF10635; HMM-score: 14.5)
    HTH (CL0123) HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 12.3)
    no clan definedDUF3819; Domain of unknown function (DUF3819) (PF12842; HMM-score: 11.9)
    P-loop_NTPase (CL0023) Sigma54_activ_2; Sigma-54 interaction domain (PF14532; HMM-score: 9.3)
    no clan definedDUF4163; Domain of unknown function (DUF4163) (PF13739; HMM-score: 8.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.096
    • Ymax_pos: 33
    • Cmax: 0.12
    • Cmax_pos: 22
    • Smax: 0.13
    • Smax_pos: 21
    • Smean: 0.067
    • D: 0.085
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYEKEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENLIVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALDLFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRKKEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYRVKLALGQVRHYQLDELLNIVDACAETDYKLKSSYMDKQLILELFILSL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]