From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1424 [new locus tag: SA_RS08035 ]
  • pan locus tag?: SAUPAN004182000
  • symbol: aroE
  • pan gene symbol?: aroE
  • synonym:
  • product: shikimate 5-dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1424 [new locus tag: SA_RS08035 ]
  • symbol: aroE
  • product: shikimate 5-dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 1628335..1629141
  • length: 807
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAATTTGCAGTTATCGGAAATCCTATTTCACATTCCTTGTCGCCCGTTATGCATAGA
    GCAAATTTTAATTCTTTAGGATTAGATGATACTTATGAAGCTTTAAATATTCCAATTGAA
    GATTTTCATTTAATTAAAGAAATTATTTCGAAAAAAGAATTAGATGGCTTTAATATCACA
    ATTCCTCATAAAGAACGTATCATACCGTATTTAGATTATGTTGATGAACAAGCGATTAAT
    GCAGGTGCAGTTAACACTGTTTTGATAAAAGATGGCAAGTGGATAGGGTATAATACAGAT
    GGTATTGGTTATGTTAAAGGATTGCACAGCGTTTATCCAGATTTAGAAAATGCATACATT
    TTAATTTTGGGCGCAGGTGGTGCAAGTAAAGGTATTGCTTATGAATTAGCAAAATTTGTA
    AAGCCCAAATTAACTGTTGCGAATAGAACGATGGCTCGTTTTGAATCTTGGAATTTAAAT
    ATAAACCAAATTTCATTGGCAGATGCTGAAAAGTATTTAGCTGAATTCGATATCGTTATT
    AATACAACACCAGCGGGTATGGCTGGAAATAACGAAAGTATTATTAATTTAAAACATCTT
    TCTCCCAATACTTTAATGAGTGATATTGTTTATATACCGTATAAAACACCTATTTTAGAG
    GAAGCAGAGCGCAAGGGAAACCATATTTATAATGGCTTAGATATGTTTGTTTACCAAGGT
    GCGGAAAGCTTTAAAATTTGGACTAATAAAGATGCTGATATTAATTCTATGAAAACAGCA
    GTTTTACAACAATTAAAAGGAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    807

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1424 [new locus tag: SA_RS08035 ]
  • symbol: AroE
  • description: shikimate 5-dehydrogenase
  • length: 268
  • theoretical pI: 5.61162
  • theoretical MW: 29865
  • GRAVY: -0.130224

Function[edit source | edit]

  • reaction:
    EC 1.1.1.25?  ExPASy
    Shikimate dehydrogenaseShikimate + NADP+ = 3-dehydroshikimate + NADPH
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAromatic amino acid familyshikimate dehydrogenase (TIGR00507; EC 1.1.1.25; HMM-score: 259.9)
    MetabolismAmino acid biosynthesisAromatic amino acid familyshikimate-5-dehydrogenase (TIGR01809; EC 1.1.1.25; HMM-score: 126.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminglutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 32.8)
    Cellular processesCellular processesSporulation and germinationdipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 20.5)
  • TheSEED:  
    Amino Acids and DerivativesAromatic amino acids and derivativesChorismate Synthesis Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25) 
    Aromatic amino acids and derivativesCommon Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25) 
    CBSS-281090.3.peg.464 Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25) 
  • PFAM:
    AA_dh_N (CL0603) Shikimate_dh_N; Shikimate dehydrogenase substrate binding domain (PF08501; HMM-score: 103.3)
    NADP_Rossmann (CL0063) Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 39.3)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 20.5)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 15)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA1112(infB)translation initiation factor IF-2  [1] (data from MRSA252)
    SA2400(mqo2)malate:quinone oxidoreductase  [1] (data from MRSA252)
    SA2022(rplQ)50S ribosomal protein L17  [1] (data from MRSA252)
    SA0352(rpsF)30S ribosomal protein S6  [1] (data from MRSA252)
    SA1499(tig)trigger factor  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.242
    • Ymax_pos: 22
    • Cmax: 0.197
    • Cmax_pos: 22
    • Smax: 0.379
    • Smax_pos: 10
    • Smean: 0.28
    • D: 0.257
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHLIKEIISKKELDGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGIGYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLNINQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPILEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]