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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1518 [new locus tag: SA_RS08550 ]
  • pan locus tag?: SAUPAN004320000
  • symbol: citZ
  • pan gene symbol?: citZ
  • synonym:
  • product: citrate synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1518 [new locus tag: SA_RS08550 ]
  • symbol: citZ
  • product: citrate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1731079..1732200
  • length: 1122
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCAGAATTACAAAGAGGTTTAGAAGGGGTTATCGCAGCGGAGACTAAAATAAGTTCA
    ATTATTGAAAGTCAATTGACTTATGCCGGCTATGATATTGATGATCTAGCTGAAAATGCG
    CAATTTGAAGAAGTTATTTTCCTATTATGGAACTATAGATTGCCAAACGAAGAAGAGCTT
    GCTCATCTAAAAGGGAAATTAAATCAATACATGACATTAAATCCTCGTGTGTATACACAT
    TTTGAGGAGTATGTTACAGATCACGTGCATCCAATGACAGCATTACGTACGTCATTATCA
    TATATTGCACATTTCGATCCTGATGCTGAAAATGAATCAGATGAAAATCGTTATGAAAGA
    GCAATGCGTATACAGGCTAAAGTAGCATCATTAGTTACAGCGTTTGCTCGAGTAAGACAA
    GATAAAGAACCACTTAAGCCTAATCCTGACTTAAGTTATGCGGCAAACTTCCTATATATG
    TTACGTGGGGAATTACCAACAGATATAGAAGTAGAAGCCTTCAATAAAGCACTTATTTTA
    CACGCTGATCATGAGTTGAACGCATCTGCATTTACAGCACGTTGTGCGGTATCATCATTG
    TCAGATATGTACTCAGGTATTGTAGCAGCTGTAGGTTCTCTGAAAGGGCCATTACATGGT
    GGTGCAAACGAACAAGTTATGACGATGTTATCTGAGATTGGGTCAATTGAAAATGTTGAT
    GCTTACTTAGATGAAAAATTTGCTAATAAAGATAAAGTAATGGGCTTTGGTCATCGTGTA
    TATAAAGATGGTGATCCTAGAGCGAAATATTTAAGAGAAATGAGCCGTCAAATTACGAAA
    GACGCTGGTCGTGAAGAATTATTTGAAATGTCAGTGAAAATGGAAAAACGTATGGCAGAA
    GAAAAAGGATTAATTCCTAATGTTGATTTTTATAGTGCGAGTGTTTATCACTGTATGGAA
    ATACCTCATGACTTATTCACGCCAATCTTTGCTGTAAGTCGTTCTGCAGGATGGATTGCT
    CATATTTTAGAACAATATAAAGATAATAGAATTATGCGTCCTAGAGCGAAATATATTGGC
    GAAACGAATCGTAAGTATATCCCGCTTGAAGAAAGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1122

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1518 [new locus tag: SA_RS08550 ]
  • symbol: CitZ
  • description: citrate synthase
  • length: 373
  • theoretical pI: 5.33842
  • theoretical MW: 42593.1
  • GRAVY: -0.416086

Function[edit source | edit]

  • reaction:
    EC 2.3.3.5?  ExPASy
    2-methylcitrate synthasePropanoyl-CoA + H2O + oxaloacetate = (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
  • TIGRFAM:
    2-methylcitrate synthase/citrate synthase II (TIGR01800; HMM-score: 490.6)
    MetabolismEnergy metabolismTCA cyclecitrate (Si)-synthase (TIGR01798; EC 2.3.3.1; HMM-score: 263.9)
    citrate (Si)-synthase, eukaryotic (TIGR01793; EC 2.3.3.1; HMM-score: 83.6)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismTCA Cycle Citrate synthase (si) (EC 2.3.3.1) 
    One-carbon MetabolismSerine-glyoxylate cycle Citrate synthase (si) (EC 2.3.3.1) 
    RespirationRespiration - no subcategoryBiogenesis of c-type cytochromes Citrate synthase (si) (EC 2.3.3.1) 
  • PFAM:
    no clan definedCitrate_synt; Citrate synthase, C-terminal domain (PF00285; HMM-score: 425.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.121
    • Ymax_pos: 32
    • Cmax: 0.154
    • Cmax_pos: 32
    • Smax: 0.128
    • Smax_pos: 21
    • Smean: 0.078
    • D: 0.104
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 15927273 NCBI
  • RefSeq: NP_374806 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEEVIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYIAHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYMLRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPLHGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSRQITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSRSAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]