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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1519 [new locus tag: SA_RS08555 ]
  • pan locus tag?: SAUPAN004321000
  • symbol: aapA
  • pan gene symbol?: aapA
  • synonym:
  • product: D-serine/D-alanine/glycine transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1519 [new locus tag: SA_RS08555 ]
  • symbol: aapA
  • product: D-serine/D-alanine/glycine transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 1732548..1733909
  • length: 1362
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCTGAAAAATTACAAAGGGAACTGAGCAATCGCCACATACAATTAATTGCAATTGGC
    GGTGCAATTGGTACAGGCTTATTCTTAGGTGCTGGTCAAACGATTGCATTAACCGGCCCT
    TCAATTCTATTAACATACATCATTATAGGATTTATGTTATTTATGTTTATGCGAGGTTTA
    GGAGAAATCATTATACAGAATACTGAATTTAAATCTTTCGCAGATGTAACCAATACATAT
    ATTGGGCCTTTTGCAGGATTTGTTACCGGATGGACATACTGGTTCTGTTGGATTATTACA
    GGTATGGCTGAAGTAACGGCTGTGGCAAAATATGTTAGCTTTTGGTTCCCAGAAATTCCA
    AACTGGATAAGTGCACTATTTTGTGTACTGTTATTAATGTCATTCAACCTACTTAGCGCA
    AGACTTTTCGGAGAATTAGAATTTTGGTTCTCTATCATTAAAATAGCGACAATTATTGGT
    TTAATAGTAGTTGGTTTCGTCATGATTCTATTTGCATTTAAAACTCAATTCGGGCATGCC
    AGTTTCACAAATTTATATGAACACGGCATATTCGCTAAAGGTGCTTCTGGATTCTTTATG
    TCTTTCCAAATGGCATTATTCTCATTTGTAGGAATTGAAATGATTGGTGTTACAGCTGGG
    GAAACAAAAGATCCAGTTAAAACAATTCCAAAGGCAATTAACAGTGTACCCATTAGAATT
    TTAATATTTTACGTTGGTGCGTTAGCGGTTATCATGTCTATTATCCCTTGGCAGCAAGTT
    GATCCTGATAACAGTCCATTCGTAAAATTATTCGCATTGATCGGAATTCCGTTTGCTGCG
    GGCTTGATTAATTTTGTAGTATTAACCGCTGCTGCTTCATCATGTAACAGTGGTATATTC
    TCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCT
    AAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTT
    GTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACT
    ACCATCTCTACAGTGTTATTTTTAGTTGTATGGGGTCTGATTATCATTGCATATATCAAT
    TATAGTCGTAAAAACCCAGATCTACATAAAAATGCTACGTACAAACTATTAGGTGGTAAA
    TATATGGGCTACTTAATATTTGTATTCTTCATTTTTGTGTTCGGGTTATTATTTATTAAT
    GTTGATACAAGACGTGCAATTTATTTTATTCCGATTTGGTTTATACTTTTAGCATTTATG
    TACTTAAGATATAAACGTATCGCTGCTAAATCAAATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1362

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1519 [new locus tag: SA_RS08555 ]
  • symbol: AapA
  • description: D-serine/D-alanine/glycine transporter
  • length: 453
  • theoretical pI: 9.92242
  • theoretical MW: 50480.7
  • GRAVY: 0.80883

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesGABA permease (TIGR01773; HMM-score: 317.9)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid permease (yeast) (TIGR00913; HMM-score: 265.7)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid transporter (TIGR00909; HMM-score: 109.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesethanolamine permease (TIGR00908; HMM-score: 81.2)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminestransporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 71.6)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminescationic amino acid transport permease (TIGR00906; HMM-score: 69.9)
    histidine-histamine antiporter (TIGR04298; HMM-score: 54.6)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesL-type amino acid transporter (TIGR00911; HMM-score: 41)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesarginine-ornithine antiporter (TIGR03810; HMM-score: 40.8)
    putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 38.8)
    putrescine-ornithine antiporter (TIGR04299; HMM-score: 18.1)
    MetabolismEnergy metabolismElectron transportcytochrome c oxidase, subunit II (TIGR02866; EC 1.9.3.1; HMM-score: 11.1)
  • TheSEED:  
    Amino Acids and DerivativesAlanine, serine, and glycineGlycine and Serine Utilization D-serine/D-alanine/glycine transporter 
    CarbohydratesCentral carbohydrate metabolismPyruvate Alanine Serine Interconversions D-serine/D-alanine/glycine transporter 
  • PFAM:
    APC (CL0062) AA_permease; Amino acid permease (PF00324; HMM-score: 339.5)
    AA_permease_2; Amino acid permease (PF13520; HMM-score: 147)
    Transporter (CL0375) Amastin; Amastin surface glycoprotein (PF07344; HMM-score: 9.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.154
    • Ymax_pos: 37
    • Cmax: 0.217
    • Cmax_pos: 37
    • Smax: 0.165
    • Smax_pos: 24
    • Smean: 0.115
    • D: 0.139
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

  • GI: 15927274 NCBI
  • RefSeq: NP_374807 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILLTYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGMAEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIGLIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVTAGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGIPFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFASSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNATYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAKSNK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • aapA no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]