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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1651 [new locus tag: SA_RS09330 ]
  • pan locus tag?: SAUPAN004757000
  • symbol: hemH
  • pan gene symbol?: hemH
  • synonym:
  • product: ferrochelatase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1651 [new locus tag: SA_RS09330 ]
  • symbol: hemH
  • product: ferrochelatase
  • replicon: chromosome
  • strand: -
  • coordinates: 1885294..1886217
  • length: 924
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACTAAAAAAATGGGGTTATTAGTTATGGCTTATGGCACACCTTATAAAGAAAGTGAC
    ATAGAGCCATATTATACAGATATTAGACATGGTAAACGTCCATCTGAAGAAGAACTTCAA
    GATTTGAAAGATAGATATGAATTTATAGGTGGTTTATCACCATTAGCAGGTACAACAGAT
    GACCAAGCTGATGCACTAGTTTCAGCATTAAATAAAGCATATGCAGATGTTGAATTTAAA
    CTATACTTAGGATTAAAACACATTTCACCATTTATCGAAGATGCGGTTGAACAAATGCAC
    AATGATGGTATTACTGAAGCAATCACGGTAGTACTAGCACCACATTATTCTTCATTTTCA
    GTAGGATCATATGACAAACGTGCTGATGAAGAAGCTGCAAAATATGGTATTCAACTTACA
    CATGTGAAACATTATTATGAACAACCTAAATTTATTGAATATTGGACGAATAAAGTCAAC
    GAAACATTAGCTCAAATACCGGAAGAGGAACATAAAGACACGGTATTAGTTGTTTCGGCA
    CATAGTTTGCCAAAAGGTTTAATCGAAAAGAATAATGATCCATATCCACAAGAACTAGAA
    CATACTGCGCTTTTAATTAAAGAACAATCTAATATTGAACATATCGCGATTGGTTGGCAA
    TCTGAAGGTAATACAGGTACACCTTGGTTAGGGCCAGATGTACAAGATTTAACACGTGAT
    TTATATGAAAAACATCAGTATAAAAACTTTATATATACGCCAGTAGGTTTTGTATGTGAG
    CATTTAGAGGTGCTTTATGACAATGATTATGAATGTAAAGTTGTTTGCGATGATATTGGT
    GCGAATTATTATCGTCCAAAAATGCCGAATACACATCCATTATTTATCGGTGCAATTGTT
    GATGAAATCAAGTCTATATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    924

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1651 [new locus tag: SA_RS09330 ]
  • symbol: HemH
  • description: ferrochelatase
  • length: 307
  • theoretical pI: 4.62858
  • theoretical MW: 35056.1
  • GRAVY: -0.49316

Function[edit source | edit]

  • reaction:
    EC 4.99.1.1?  ExPASy
    Protoporphyrin ferrochelataseProtoheme + 2 H+ = protoporphyrin + Fe2+
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminferrochelatase (TIGR00109; EC 4.99.1.1; HMM-score: 269.3)
  • TheSEED:  
    Biogenesis of c-type cytochromes Coproporphyrin ferrochelatase (EC 4.99.1.-) 
    CPO analysis Coproporphyrin ferrochelatase (EC 4.99.1.-) 
  • PFAM:
    Chelatase (CL0043) Ferrochelatase; Ferrochelatase (PF00762; HMM-score: 266.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.12
    • Ymax_pos: 11
    • Cmax: 0.148
    • Cmax_pos: 66
    • Smax: 0.219
    • Smax_pos: 1
    • Smean: 0.127
    • D: 0.123
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKDRYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHNDGITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVNETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIGWQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVCDDIGANYYRPKMPNTHPLFIGAIVDEIKSIF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SA1678 (PerR*)
    PerR* (TF) important in Oxidative stress responseRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]