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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2212 [new locus tag: SA_RS12695 ]
  • pan locus tag?: SAUPAN005964000
  • symbol: SA2212
  • pan gene symbol?: bioF
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2212 [new locus tag: SA_RS12695 ]
  • symbol: SA2212
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2482581..2483696
  • length: 1116
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGATGTTTCAAGAGCAAATTGATTTATTACAACAGAAAGGGTTATATCGGTCGCTTAAA
    TCGGTAGGGTATATAGATAGACAGTATATTGAAGTAGAAAATAAGAGATGTACGAACTAT
    ACATCTAATGATTATTTAGGATTAGGTCAAATAGCGTTTGATAAGGATGATTTCGAAAGA
    TTTATGCGGAAGTATAGTTATCACTTATCAAGTTCAAGATTAATTAGTGGAAGTTCGACA
    GCTTATGAAGAAATTGAAACAATGTTAGCAGGTTGGCTCGGATATAGTGCATGTACTATC
    TTAAATAGTGGTTATGATGCTAATTTGGCGTTGTTTAATATTTTCAAAAATACAAATTGT
    GTCGTGTTTTCAGATCAAGAAAATCATGCGAGTATTATTGACGGTATTAAGTTAAGTGGT
    TTAGAAAAAGTGATATATAAGCATTTAGATATTGCTGATTTAGAAAAAAGGTTAGAGAAA
    TACCCTAATCAAAATATACCAAAAATAATCATATCTGATAGTGTATTTTCAACGAATGGC
    GACGTTGTGGATATTGGTCAATTAGTCTCATTAAAGCATAAATATAACGCAACACTGATA
    CTCGATGTTTCACATAGTTTTGGAATAGAGAATTACTCGAATTATCAAGGTGTAGATATA
    CTCACTTCTAGTTTATCTAAAGCATGTGGTGCATACGGGGGTGTGATTTTAAGTTCAAAT
    GATGTGAAGGATATGTTAATTAATCACGGTAGACCACTCATCTACTCAAGTAGTTTGCCA
    ATTTATAATTTGTATTTTATAAAAAGAAACATTGAAAAGTTAATAAATGCTGATGATAGA
    CGCACTAAATTAAATAGTTTGAGTAAATATTTTAACCAAAAGTTAAAAGCACTCAATGTT
    AATTATAATAGTTCAAACTCGCCGATTAAGTTTATTGAGTTTGATGACATAGAAGCGGCT
    GAGAACATTCATCAAACATTATTAAAGCATCATGTGTTTACAAGTTATTTAAGGTATCCA
    ACTGTGACTAAGCCAATGTTAAGAATATCATTGTCGTATTTTCATACTGAACAAGATATA
    GATAGGTTGTTTGAAATTTTGCATCAAGAAGATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1116

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2212 [new locus tag: SA_RS12695 ]
  • symbol: SA2212
  • description: hypothetical protein
  • length: 371
  • theoretical pI: 6.58668
  • theoretical MW: 42564
  • GRAVY: -0.298922

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotin8-amino-7-oxononanoate synthase (TIGR00858; EC 2.3.1.47; HMM-score: 271.5)
    putative pyridoxal phosphate-dependent acyltransferase (TIGR01825; EC 2.3.1.-; HMM-score: 219.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalamin5-aminolevulinic acid synthase (TIGR01821; EC 2.3.1.37; HMM-score: 185.2)
    MetabolismEnergy metabolismAmino acids and aminesglycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 182.8)
    Unknown functionEnzymes of unknown specificitycysteine desulfurase family protein (TIGR01977; HMM-score: 25)
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase (TIGR03588; EC 2.6.1.92; HMM-score: 23.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase, SufS family (TIGR01979; HMM-score: 22.1)
    MetabolismAmino acid biosynthesisAspartate familyO-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 14.6)
    MetabolismAmino acid biosynthesisSerine familyO-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 14.6)
    MetabolismAmino acid biosynthesisAspartate familyO-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 14.2)
    2-aminoethylphosphonate aminotransferase (TIGR03301; EC 2.6.1.-; HMM-score: 11.8)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsBiotinBiotin biosynthesis 8-amino-7-oxononanoate synthase (EC 2.3.1.47) 
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 138.6)
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 35.3)
    Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 24.9)
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 20.1)
    PDDEXK (CL0236) Cas_DxTHG; CRISPR-associated (Cas) DxTHG family (PF09455; HMM-score: 17.7)
    Peptidase_CA (CL0125) Peptidase_C10; Peptidase C10 family (PF01640; HMM-score: 14.9)
    PLP_aminotran (CL0061) DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 14.1)
    TIM_barrel (CL0036) DAHP_synth_1; DAHP synthetase I family (PF00793; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.111
    • Ymax_pos: 18
    • Cmax: 0.106
    • Cmax_pos: 55
    • Smax: 0.167
    • Smax_pos: 9
    • Smean: 0.116
    • D: 0.113
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSNDYLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILNSGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEKYPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGVDILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLINADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFTSYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SA1289 (BirA*)
    BirA* (TF) important in Biotin biosynthesisRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]