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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2228 [new locus tag: SA_RS12785 ]
  • pan locus tag?: SAUPAN005988000
  • symbol: SA2228
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2228 [new locus tag: SA_RS12785 ]
  • symbol: SA2228
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2499585..2501663
  • length: 2079
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCACTATTAGAAGCATTTTTAATTTTTATTTTTGCTGTGATTATTAGTTCGGTTATC
    AATAATCGATTCCCACAAATTCCTACTGCCTTTATTCAAATTGCATTAGGTGTCGTTATT
    TTCATTATTCCAATACAAGTTGATTTCCAATTCAATTCTGAAGTGTTTATGTTTGCCGTT
    ATCGCGCCACTACTTTTTGTAGAAGGTACCCACGTCTCTCGAACAAAATTATTAGAATAT
    CGTAAACCTATACTATTAATGTCAATGGCACTAGTGTTTGCAACTGTAGTCGGTGTTGGC
    TATTTTATACATTGGATTTGGCCTGCCTTACCAATGCCAGCCGCTTTTGCAATAGCAGCA
    ATTTTATGTCCTACTGATGCAGTAGCAGTATCTGCTATTACACGCGGAAAATTATTACCT
    AAAGGTTCTATGACGATTTTAGAAGGTGAATCTTTACTCAATGATGCAGCAGGTATCATT
    TCATTTAAAATTGCTGTTACTGCATTAGTAACTGGTACCTTTTCATTATTCCAAGCTGTT
    GAACAATTTATCATTTCAACAATACTAGGTGTACTTATTGGTGCAATTATTGGATTCGTT
    GTCGTTAGAATACGTATAGACTTAACCGCTAATAAAGGTTTAAAAGATAACAATACCTTA
    ACTTTTATTCAATTATTAACTCCTTTTGTCGTATACTTTTTAGCAGAAGAAGTTCATGCT
    TCAGGTATCATTGCAGTTGTAATCGCAGGTCTAATCCATGGTTTAGAACGCGACCGTTTA
    ATTAGAGCACAAACGGAACTACAAATGAATTACCATCAAATTTGGAACACATTTAGTTAT
    GCCTTAAATGGTTTTGTATTTGTTGTATTAGGTTTTATGATTCCTACTGTAGTCATAGAT
    ATTTTCCAAACAGAGCCTGACAACTTTTCATTTTTAATAGTGATCACTATATTAATCGCT
    ATTGCAATTTATGCATGTCGATTTGTTTGGGTTTATTTCTGGTACAAAGATTTTTATTTC
    CCGAAAAATATACAATCTTATCTAGACGAAGAACATGATTCACATGAAACACCACCTTCT
    CGTGTGCGTTACGCATTTATTATGACGATGTGTGGTATTCACGGTACAATTTCACTTTCA
    ATGGCACTTACATTACCATTTATCATTACAAAAGGACAAGCATTCGAATACCGTAATGAT
    TTATTGTTTATTGCATCTTTCATGGTATTAATTAGTTTAATCTTAGCGCAAATTGTTTTA
    CCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCC
    AAAATTTTCATTGTTCAAAAAGTGATACAGCATTTTAAAAACGAAAGTAAAAAAGACAAA
    AACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTA
    AATTCAGAACCTAATAATCAAAATACAAAAGAACTCAAACGTTTAGAAGATATTGCAAAA
    GTAATCGAAACATCTACACTTGAGCGTTTAATTGATAAAGGTAAGGCAACGTATCAGGAT
    ATTAATAATTACCGCAATATTGTCGAATTAACAGAGACACACCGTACTGCATCATTTGTT
    GAAAAATTTGCAAACTTCTTCAAGATGTTATGGCTTAGACTTAAAGCGATGAAGCACTAC
    AAAGCCTTAAACAAAGAATCTAAGAAGCAAGAATTTGAAAATTCATTCAAAGATGTTCAA
    AAAATTATGCGTATTGTGAATCACAATATTATTTTACGCTTAAAAGAAGAACAAAATAGT
    ACAAATGTACTTGAGGTTAGCTTAGTCATTAATCATTACTATGATATGAGTCGCTCATTA
    AAGTGGCGTGCACAACGTCGAAAAGAACGTCAAGAAAACAGCAATCAAATCATACCGCAA
    GCTATGTTCCATAACCACAAATTGGAAGCATTGTACTTACAACGTCACCTTTTAGATGAA
    TTAATTCGCAAAAATAAAATCAACAATATCGTTGCAGCTCAAATTCGAGAAAATATCAAT
    TACAACGAAATTGTCTTGTCTTTACAGTCCAAACATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2079

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2228 [new locus tag: SA_RS12785 ]
  • symbol: SA2228
  • description: hypothetical protein
  • length: 692
  • theoretical pI: 9.32356
  • theoretical MW: 79338.5
  • GRAVY: 0.276734

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsNa+/H+ antiporter (TIGR00831; HMM-score: 250.5)
    MetabolismTransport and binding proteinsCations and iron carrying compoundssodium/hydrogen exchanger 3 (TIGR00840; HMM-score: 56.7)
  • TheSEED:  
    Na+/H+ antiporter 
  • PFAM:
    CPA_AT (CL0064) Na_H_Exchanger; Sodium/hydrogen exchanger family (PF00999; HMM-score: 173.8)
    no clan definedDUF2157; Predicted membrane protein (DUF2157) (PF09925; HMM-score: 16.5)
    DUF3139; Protein of unknown function (DUF3139) (PF11337; HMM-score: 12.9)
    Phage_holin_3_6; Putative Actinobacterial Holin-X, holin superfamily III (PF07332; HMM-score: 10.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 0
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.149
    • Ymax_pos: 26
    • Cmax: 0.147
    • Cmax_pos: 26
    • Smax: 0.215
    • Smax_pos: 7
    • Smean: 0.163
    • D: 0.155
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIPIQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYFIHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGIISFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNNTLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWNTFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFWYKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKGQAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQHFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPNNQNTKELKRLEDIAKVIETSTLERLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKESKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRAQRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYNEIVLSLQSKH

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]