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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2254 [new locus tag: SA_RS12945 ]
  • pan locus tag?: SAUPAN006026000
  • symbol: opp-1B
  • pan gene symbol?: cntB
  • synonym:
  • product: oligopeptide transporter permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2254 [new locus tag: SA_RS12945 ]
  • symbol: opp-1B
  • product: oligopeptide transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 2530507..2531442
  • length: 936
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTCAAATTTATCTTAAAACGTATTGCGCTCATGTTTCCATTAGTGATTGTAGTAAGT
    TTTATGACATTTCTATTGACGTATATTACAAATGAAAATCCAGCTGTGACAATTTTACAT
    GCACAAGGAACGCCAAATGTAACACCAGAGTTGATTGCAGAAACGAATGAAAAGTACGGT
    TTCAATGATCCATTATTAATTCAATATAAAAACTGGTTACTTGAAGCGATGCAATTTAAT
    TTTGGTACAAGTTACATTACAGGGGATCCTGTTGCTGAACGCATTGGCCCAGCATTTATG
    AACACATTGAAATTAACAATAATTTCAAGTGTTATGGTGATGATTACATCAATTATTTTA
    GGTGTAGTTAGTGCATTAAAAAGAGGAAAGTTCACTGATCGTGCGATACGTTCAGTGGCT
    TTCTTTCTAACTGCATTACCATCATATTGGATAGCTTCAATACTTATTATTTACGTTTCA
    GTGAAGTTAAACATATTGCCGACTTCTGGATTAACAGGCCCAGAAAGTTACATATTGCCA
    GTGATCGTTATTACGATTGCCTATGCTGGTATTTACTTTAGAAATGTTAGACGCTCGATG
    GTGGAACAATTAAATGAAGATTATGTACTTTATTTAAGAGCAAGCGGTGTGAAATCTATC
    ACATTAATGTTGCATGTGTTGCGTAATGCTATACAAGTTGCGGTATCAATCTTTTGTATG
    TCTATACCAATGATAATGGGTGGACTAGTTGTTATCGAGTATATCTTTGCATGGCCTGGA
    CTAGGTCAATTAAGTTTAAAAGCAATACTTGAACACGATTTTCCAGTCATTCAAGCATAT
    GTATTAATTGTAGCGGTATTATTTATTGTATTTAATACATTAGCAGATATCATTAATGCG
    CTATTAAATCCAAGATTAAGGGAGGGCGCACGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2254 [new locus tag: SA_RS12945 ]
  • symbol: Opp-1B
  • description: oligopeptide transporter permease
  • length: 311
  • theoretical pI: 9.51657
  • theoretical MW: 34748.3
  • GRAVY: 0.755949

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsnickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 285.4)
    MetabolismTransport and binding proteinsAnionsphosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 10.8)
  • TheSEED:  
    Nickel transport system permease protein NikB (TC 3.A.1.5.3) 
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 141)
    no clan definedDUF4133; Domain of unknown function (DUF4133) (PF13571; HMM-score: 9.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide length 12 aa
    • Ymax: 0.274
    • Ymax_pos: 12
    • Cmax: 0.169
    • Cmax_pos: 36
    • Smax: 0.826
    • Smax_pos: 8
    • Smean: 0.771
    • D: 0.468
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

  • GI: 15928045 NCBI
  • RefSeq: NP_375578 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MFKFILKRIALMFPLVIVVSFMTFLLTYITNENPAVTILHAQGTPNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNTLKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVSVKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVKSITLMLHVLRNAIQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPVIQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Laetitia Remy, Marie Carrière, Aurélie Derré-Bobillot, Cécilia Martini, Maurizio Sanguinetti, Elise Borezée-Durant
The Staphylococcus aureus Opp1 ABC transporter imports nickel and cobalt in zinc-depleted conditions and contributes to virulence.
Mol. Microbiol.: 2013, 87(4);730-43
[PubMed:23279021] [WorldCat.org] [DOI] (I p)