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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2294 [new locus tag: SA_RS13155 ]
  • pan locus tag?: SAUPAN006131000
  • symbol: gntK
  • pan gene symbol?: gntK
  • synonym:
  • product: gluconokinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2294 [new locus tag: SA_RS13155 ]
  • symbol: gntK
  • product: gluconokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2576949..2578502
  • length: 1554
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAATATATGATTGGTGTCGACATTGGAACGACGAGTACCAAGTCAGTCTTATATGAT
    GAAAATGGAGCTTTTATCATGAAACATCAAATCGGCTATGATTTACACACACCAAACGTT
    GATGTCTCAGAAGAAAACCCAGATGAATTATTTGATGCGGTATTAATGACAATTAAATAC
    ATAATGAGGGAATCGAAAGTTAATCAAGATGATATTAAATTTGTGTCATTTAGTGCGCAA
    ATGCATAGCTTGATTGCGATGGATCAGCAACATCAAAGATTAACAAATAATATTACTTGG
    GCAGATAACCGCGCTGCAAAATATGCAACAGTAATAAATGAAGTGCATGATGGCAATGCG
    ATTTATCAGCGAACAGGTACGCCTATTCATCCTATGTCGCCTTTAGTGAAAATTTTTTGG
    ATGAAACATGAATGGCAAGATGTATTTCAACGTACTGCTAAGTTTGCAGATATTAAAACA
    TACATTTTCTATCATTTATTTGATACATATATCATTGATTATTCAATGGCTTCCGCAACA
    GGGATGTTTAATTTAGAAACATTAGATTGGGATGTTGGGGCATTAGAATTGCTTGGTATT
    TCCAAGGAAATGTTCCCGGAATTAGTGCCAACAACGTACGTAATGAAAGGCATGAAAGAA
    CGTTATGCAACATTAATGGGGCTTAATGAAGATACACCGTTTGTTATTGGTGCGAGTGAT
    GGGGTCCTTTCTAATTTGGGTGTCAATAGTGTTGGTAAAGGAGAAGTTGCTGTCACAATC
    GGTACATCTGGCGCGATTCGTACTGTGATAGATAAACCACGTACTGATTACAAAGGTAGA
    ATATTTTGTTATGTCTTAACAGAGGACCACTACGTCATCGGAGGTCCTGTAAATAATGGT
    GGTGTCGTATTGAGATGGTTGCGCGACGAGTTGCTAGCGAGTGAAGTCGAAACTGCGAAA
    CGTCTCGGTGTTGATCCTTATGATGTCTTAACTCAAATTGCAAAGCGTGTTAAACCAGGT
    GCAGATGGTTTAATATTCCATCCCTATTTAGCTGGAGAACGTGCGCCGCTTTGGAACGCA
    AATGCAAGAGGTTCATTCTTCGGTTTAACTTTATCTCATAAAAAAGAACATATGATTCGC
    GCTGCATTAGAAGGTGTTCTTTACAATTTGTATACCGTCTACCTTGCACTCATTGAAGTA
    ATGAATGAAACGCCTAATATGATAAAAGCAACAGGTGGTTTTGCGAAAAGTGAAGTATGG
    CGTCAAATGATGTCAGATATATTTGACACAGAGTTAGTGGTTCCTGAAAGTTATGAAAGT
    TCATGCTTAGGTGCCTGCGTGCTTGGACTTAAAGCTGTAGGTGACATTGAAGATTTTTCA
    ATCGTTTCATCGATGGTCGGTGCTACAAATAATCATACGCCGTTTGAAGAAAATGTCGCT
    GTTTACCAAGAGCTCGTATCCATTTTTATCAATTTAAGTCGTTCTTTAACAGAGAATTAT
    GAACAAATTGCAGATTTTCAACGCCAACATATGGCTGAAAATAAAACACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1554

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2294 [new locus tag: SA_RS13155 ]
  • symbol: GntK
  • description: gluconokinase
  • length: 517
  • theoretical pI: 4.97775
  • theoretical MW: 58077
  • GRAVY: -0.109284

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismEnergy metabolismSugarsgluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 1004.5)
    MetabolismEnergy metabolismSugarsxylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 332.1)
    MetabolismEnergy metabolismOtherglycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 205.3)
    MetabolismEnergy metabolismSugarsL-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 119)
    FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 95.9)
    MetabolismEnergy metabolismSugarsribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 63)
    rhamnulokinase (TIGR02627; EC 2.7.1.5; HMM-score: 60.7)
    putative CoA-substrate-specific enzyme activase (TIGR00241; HMM-score: 21)
    benzoyl-CoA reductase, subunit A (TIGR02259; EC 1.3.7.8; HMM-score: 11)
  • TheSEED:  
    CarbohydratesMonosaccharidesD-gluconate and ketogluconates metabolism Gluconokinase (EC 2.7.1.12) 
  • PFAM:
    Actin_ATPase (CL0108) FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 238.7)
    FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 108.2)
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 24.9)
    no clan definedDDE_Tnp_IS66_C; IS66 C-terminal element (PF13817; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.119
    • Ymax_pos: 25
    • Cmax: 0.131
    • Cmax_pos: 25
    • Smax: 0.197
    • Smax_pos: 2
    • Smean: 0.11
    • D: 0.115
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 15928085 NCBI
  • RefSeq: NP_375618 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKYMIGVDIGTTSTKSVLYDENGAFIMKHQIGYDLHTPNVDVSEENPDELFDAVLMTIKYIMRESKVNQDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWADNRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLVKIFWMKHEWQDVFQRTAKFADIKTYIFYHLFDTYIIDYSMASATGMFNLETLDWDVGALELLGISKEMFPELVPTTYVMKGMKERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTDYKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIAKRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYTVYLALIEVMNETPNMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGLKAVGDIEDFSIVSSMVGATNNHTPFEENVAVYQELVSIFINLSRSLTENYEQIADFQRQHMAENKTQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: CcpA, GntR
    CcpA (TF) important in Carbon catabolism  RegPrecise
    GntR (TF) important in Gluconate utilization  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol. Microbiol.: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] (P p)

Relevant publications[edit source | edit]