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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2365 [new locus tag: SA_RS13550 ]
  • pan locus tag?: SAUPAN006253000
  • symbol: SA2365
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2365 [new locus tag: SA_RS13550 ]
  • symbol: SA2365
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2655134..2655838
  • length: 705
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGACTAAAATTGTGTTAATCACAGGAGGCAATAAAGGGTTAGGCTATGCAAGTGCAGAG
    GCACTTAAAGCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAA
    CAAGCATCACAAAAATTAGGTGTTCATTATGTACAGTTAGATGTTACAAGTGACTACTCA
    GTCAAAAATGCTTATAACATGATTGCTGAAAAAGAAGGCCGTCTTGATATCCTCATTAAC
    AATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAA
    GAAGTATATCAAACGAATGTATTCGGTATCGTACGAATGATGAATACATTCGTCCCTCTC
    TTAGAAAAATCTGAACAACCTGTTGTCGTCAACGTATCAAGTGGTTTAGGTTCATTTGGA
    ATGGTTACAAACCCCGAAACAGCTGAATCTAAAGTGAATTCATTAGCTTATTGTTCGTCT
    AAATCGGCAGTAACAATGCTAACGCTACAATACGCTAAAGGATTACCTAACATGCAAATC
    AATGCTGCTGACCCAGGCGCTACAAATACTGATTTAGTTGGTGATTTTAGTAATAATTCC
    AAACACGTTTCTGAAGGAATCAAGCCAATCATTCAATTAGCAACGATTGGTGCAGATGGC
    CCAACAGGTACATTTATTAATGGCGATGGAGAAATGCCTTGGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    705

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2365 [new locus tag: SA_RS13550 ]
  • symbol: SA2365
  • description: hypothetical protein
  • length: 234
  • theoretical pI: 5.80622
  • theoretical MW: 24931.1
  • GRAVY: -0.126923

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismFatty acid and phospholipid metabolismBiosynthesis3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 79.9)
    2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 79)
    Unknown functionEnzymes of unknown specificitySDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 69)
    MetabolismEnergy metabolismFermentationacetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 64.8)
    acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 64.1)
    3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 62.9)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharides2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 58.1)
    cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 49.1)
    Unknown functionEnzymes of unknown specificitySDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 47.3)
    2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 42.7)
    rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 36.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphylllight-dependent protochlorophyllide reductase (TIGR01289; EC 1.3.1.33; HMM-score: 34.4)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisputative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 33.3)
    sepiapterin reductase (TIGR01500; EC 1.1.1.153; HMM-score: 26.4)
    pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 16)
    Unknown functionEnzymes of unknown specificityputative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 13.5)
    Hypothetical proteinsConservedTIGR01777 family protein (TIGR01777; HMM-score: 12.1)
  • TheSEED:  
    Fatty Acid Biosynthesis FASII Short-chain dehydrogenase 
  • PFAM:
    NADP_Rossmann (CL0063) adh_short; short chain dehydrogenase (PF00106; HMM-score: 135.4)
    adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 93)
    KR; KR domain (PF08659; HMM-score: 39.4)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 17.6)
    ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 15.1)
    no clan definedPALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 14.5)
    GT-B (CL0113) Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 13.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.161
    • Ymax_pos: 20
    • Cmax: 0.128
    • Cmax_pos: 43
    • Smax: 0.304
    • Smax_pos: 15
    • Smean: 0.196
    • D: 0.175
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQKLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEVYQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSSKSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGADGPTGTFINGDGEMPW

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]