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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2396 [new locus tag: SA_RS13715 ]
  • pan locus tag?: SAUPAN006300000
  • symbol: SA2396
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2396 [new locus tag: SA_RS13715 ]
  • symbol: SA2396
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2684344..2685792
  • length: 1449
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGAAGTTTTTTCAATAAAATAGCACGAAAAGAGGATCCGGCTATCTATCAAAATAAA
    GATGGTCATTTAAAGCGTACACTTCGGGTGCGTGATTTCTTAGCTTTAGGTGTAGGAACA
    ATTGTATCGACATCTATCTTTACGCTACCTGGCATTGTTGCTGCAGAACATGCAGGACCG
    GCCGTTGCGTTATCATTCTTACTCGCTGCTATTGTTGCTGGTTTAGTTGCATTTACTTAT
    GCAGAAATGGCTGCCGCTATGCCATTTGCAGGTTCAGCCTATTCTTGGGTCAATGTATTA
    TTTGGTGAATTTTTTGGATGGGTTGCCGGTTGGGCTCTATTAGCTGAATATTTTATCGCC
    GTAGCCTTTGTTGCATCAGGATTCTCAGCGAATTTACGCGGACTTGTGAAACCAATTGGC
    ATCGAATTACCTGCAGCATTATCAAATCCATTTGGTACGAATGGCGGTTTTATCGATATT
    ATTGCTGCTATCGTTATTTTATTAACTGCATTATTACTATCACGTGGTATGTCAGAAGCA
    GCACGTATGGAAAACATTTTAGTTATTTTAAAAGTATTAGCTATTATTTTATTTGTCATC
    GTAGGTTTAACAGCAATAAATGTTAGTAACTATGTGCCATTTATTCCAGAACACAAAGTA
    ACTGCTACAGGTGACTTTGGTGGATGGCAAGGCATATATGCTGGTGTTTCAATGATTTTC
    TTAGCGTATATCGGTTTCGATTCTATCGCAGCAAACTCAGCAGAAGCACTTGACCCTCAA
    AAGACAATGCCTAGAGGTATTCTTGGTTCTTTAAGTGTTGCTATCGTATTATTTATTGCT
    GTAGCACTTGTGTTAGTTGGTATGTTCCATTACTCACAATACGCAAACAATGCTGAACCT
    GTTGGTTGGGCTTTACGTCAAAGTGGTCATGGTGTTGTAGCAGCTATTGTTCAAGCTATC
    TCTGTTATCGGTATGTTTACAGCATTAATTGGTATGATGTTAGCAGGCTCACGTTTACTT
    TATTCATTTGGACGTGACGGCTTATTACCTTCATGGTTAAGCCATTTAAACGACAAACAT
    TTACCTAATCGCGCACTAGTTATACTTACTATTATTGGTGTTTTAATTGGTTCTATGTTC
    CCATTCGCATTTTTAGCACAATTAATTTCAGCAGGTACACTTGTTGCATTTATGTTCGTT
    TCATTAGCAATGTATCGTTTGAGAAAGCGTGAAGGTAAAGATCTACCAATTCCTGCATTT
    AAATTACCTTTATATCCTGTGTTACCAGCAATTACATTTGTTTTAGTATTGCTAGTATTC
    TGGGGATTAGGATTCGAAGCGAAATTATACACTTTAATTTGGTTTATTGTTGGTATCATT
    CTATATTTAAGTTATGGACTTAGACATTCTAAGAAAAATGATGTAGCTGAATACCACCCA
    CCAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1449

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2396 [new locus tag: SA_RS13715 ]
  • symbol: SA2396
  • description: hypothetical protein
  • length: 482
  • theoretical pI: 9.73071
  • theoretical MW: 51893.3
  • GRAVY: 0.837344

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid transporter (TIGR00909; HMM-score: 325.6)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminescationic amino acid transport permease (TIGR00906; HMM-score: 252.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminestransporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 114.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesethanolamine permease (TIGR00908; HMM-score: 112.6)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesL-type amino acid transporter (TIGR00911; HMM-score: 90.6)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid permease (yeast) (TIGR00913; HMM-score: 80.4)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesarginine-ornithine antiporter (TIGR03810; HMM-score: 76.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesGABA permease (TIGR01773; HMM-score: 68.4)
    permease, urea carboxylase system (TIGR03428; HMM-score: 66.4)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid permease (TIGR00907; HMM-score: 50.2)
    histidine-histamine antiporter (TIGR04298; HMM-score: 42.2)
    putrescine-ornithine antiporter (TIGR04299; HMM-score: 36)
    putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 31)
    MetabolismTransport and binding proteinsOtherK-Cl cotransporter (TIGR00930; HMM-score: 2.1)
  • TheSEED:  
    Uncharacterized amino acid permease, GabP family 
  • PFAM:
    APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 194.5)
    AA_permease; Amino acid permease (PF00324; HMM-score: 129.1)
    AA_permease_C; C-terminus of AA_permease (PF13906; HMM-score: 29.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.341
    • Ymax_pos: 55
    • Cmax: 0.367
    • Cmax_pos: 55
    • Smax: 0.399
    • Smax_pos: 51
    • Smean: 0.166
    • D: 0.273
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVSTSIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFGEFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDIIAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEHKVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIVLFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMMLAGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAGTLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLYTLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA2396 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]