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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2402 [new locus tag: SA_RS13745 ]
  • pan locus tag?: SAUPAN006309000
  • symbol: SA2402
  • pan gene symbol?:
  • synonym:
  • product: acetate-CoA ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2402 [new locus tag: SA_RS13745 ]
  • symbol: SA2402
  • product: acetate-CoA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 2691272..2692891
  • length: 1620
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTATGAAAGGAAGTTAGTTATGCAAAAGTCAGCTCTATTCGCGCCTGAAAAATATAAC
    ATTATTTCGGAAATCGAAAAATATAGCCACATACCAGATAAAAAGGCAATTTTATATCAT
    AATACTGAAGGCGAAGATATATCAGTCACTTACCAACAGCTCATTGAACAGTCTAATAAA
    GTAGGTAATGTTTTAGCATCGCACGGTTTATCTAAAGGCGATAAAGTACTCATTATGATG
    CCACGATCTATTGCGACGTATGAATTATATATTGCTGCTTTAAAATTAGGTGTTGCAATC
    ATTCCATGTTCTGAAATGCTAAGAACTAAAGATTTACAATACCGTATCACGCACGGTGAA
    ATAAATGCAGTTATTGCACTAGAGCCATTCACAGTTGAATTTGAAAAAATTAAAGAATAT
    GATGCGTTAACTAAATTTGTTATTGCCGGTCAAAAAGATGGATGGATTTCACTTGAAGCT
    GAAAAAGAAAACGCAAGTAACAAACTAGAAATGGCAGATACGACGCGTGATGATATGGCG
    ATTCTGTCATATACATCTGGTACAACTGGCAATCCTAAAGCCGTTACACATTGTCATGGA
    TGGGGATTTGCACATTTACAAATGGCACCAAAACATTGGTTATGTATTAATGAAGATGAC
    TTAGTTTGGGCAACAGCAGCACCAGGTTGGCAAAAATGGGTATGGAGTCCTTTCTTATCT
    GTATTAGGTTCAGGTGCAACTGCTTTTGTATTTAATGGAAGGTTCAGTCCTGAAACATAC
    TTAGAATTGCTTCAAAAATATCAAATTAATGTTTTATGTTGTACACCGACAGAATATCGT
    ATGATGGCTAAATTACAGAACTTAAATGATTATGACTTAACGCATTTACATAGTGCCGTT
    TCTGCAGGTGAGCCACTAAATCGAGAAGTAGTTGAACAATTCAAGAAATACTTTAATTTA
    ACTGTAAGAGATGGATATGGCCAGACTGAAAGTACCTTGCTTATAGGCTTTTTAAAAGAT
    ACACCACAGAGGATTGGATCAATGGGTAAAGGCATTCCAGGTAGTTCGGTCACTGTAGTA
    GATGATGAGGGGAATAGCGTACCTGCTAATACGAAAGGGAATATTGCAGTACCACTTGAT
    TTGCCAGCACTTTTCAAAGGGTATTATAAAGAGCCAGAACGTACGGCGAAAGCGCAAACA
    GGTAAATACTACATCACTGGTGACTTAGCTCATGTAGATGATGATGGTTATTTCTGGTTT
    GAAGGACGTCGAGATGACATTATTATTAGTTCAGGCTATACGATTGGGCCTTTTGAAGTT
    GAAGATGCGTTAACGAATCATCCAGCCGTTAAAGAATGTGCTGTAGTAGCAAAACCTCAT
    GATATACGAGGAAATATTGTTAAAGCATTCGTTATTTTACAGGACCATACTGCGGGTGAT
    GATACGTTAGTTAAAGAACTACAACAATTTGTTAAAAATGAAGTTGCACCATATAAATAT
    CCGAGAGAGATTGAATTCGTTGACGACTTACCAAAAACAAACTCAGGTAAAATTCGTCGT
    GTTGAACTACGAGATTCTGAAATAGAAAAATGGCAACAACAAAAAGATAGCAATCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2402 [new locus tag: SA_RS13745 ]
  • symbol: SA2402
  • description: acetate-CoA ligase
  • length: 539
  • theoretical pI: 5.64153
  • theoretical MW: 60684.6
  • GRAVY: -0.355844

Function[edit source | edit]

  • reaction:
    EC 6.2.1.1?  ExPASy
    Acetate--CoA ligaseATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
  • TIGRFAM:
    benzoate-CoA ligase family (TIGR02262; HMM-score: 295.3)
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 287.4)
    amino acid adenylation domain (TIGR01733; HMM-score: 185)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneO-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 180.7)
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 176.9)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 176.4)
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 175.7)
    MetabolismTransport and binding proteinsCations and iron carrying compounds(2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 167.7)
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 160.6)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanD-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 145.9)
    MetabolismCentral intermediary metabolismOtheracetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 109)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 49.4)
    L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 33.6)
    MetabolismEnergy metabolismOtherphenylacetate-CoA ligase (TIGR02155; EC 6.2.1.30; HMM-score: 18.9)
    TIGR03089 family protein (TIGR03089; HMM-score: 12.9)
  • TheSEED:  
    Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases, YtcI homolog 
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 280.6)
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 89.2)
    no clan definedAtu4866; Agrobacterium tumefaciens protein Atu4866 (PF11512; HMM-score: 13.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA1084(rplS)50S ribosomal protein L19  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.139
    • Ymax_pos: 11
    • Cmax: 0.12
    • Cmax_pos: 67
    • Smax: 0.293
    • Smax_pos: 1
    • Smean: 0.167
    • D: 0.15
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MYERKLVMQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASNKLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDSEIEKWQQQKDSNQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]