From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2417 [new locus tag: SA_RS13860 ]
  • pan locus tag?: SAUPAN006337000
  • symbol: SA2417
  • pan gene symbol?: nsaS
  • synonym: braS
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA2417 [new locus tag: SA_RS13860 ]
  • symbol: SA2417
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2713024..2713911
  • length: 888
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGACCTTTCTTAAAAGTATTACTCAGGAAATAGCAATAGTCATAGTTATTTTTGCTTTA
    TTTGGCTTAATGTTTTACCTGTATCATTTGCCATTAGAAGCATATTTACTAGCACTTGGC
    GTTATTTTATTATTATTACTCATATTCATAGGTATTAAATATTTAAGTTTTGTAAAAACT
    ATAAGCCAACAACAACAAATTGAAAACTTAGAAACTGCGTTGTATCAGCTTAAAAATGAA
    CAAATTGAATATAAAAATGATGTAGAGAGCTACTTTTTAACATGGGTACATCAAATGAAA
    ACACCCATTACTGCAGCACAACTGTTACTTGAAAGAGATGAGCCTAATGTTGTTAATCGT
    GTTCGTCAAGAGGTTATTCAAATTGATAACTATACAAGTTTAGCACTTAGTTATTTAAAG
    TTATTAAATGAAACTTCTGATATTTCTGTCACTAAAATTTCGATTAATAATATCATTCGC
    CCAATTATTATGAAATATTCAATACAGTTTATTGATCAAAAAACAAAAATCCATTATGAA
    CCTTGTCATCACGAAGTATTAACTGACGTTAGATGGACCTCTTTAATGATAGAACAATTA
    ATAAATAATGCACTTAAGTATGCGAGAGGTAAAGATATATGGATTGAATTTGATGAGCAA
    TCCAATCAATTACACGTAAAAGATAATGGTATCGGTATTAGTGAAGCGGACTTGCCTAAA
    ATATTTGATAAGGGCTATTCAGGTTATAATGGCCAGCGCCAAAGTAACTCAAGTGGGATT
    GGTTTATTTATCGTAAAACAAATTTCAACACACACAAACCATCCTGTTTCAGTCGTATCT
    AAACAAAATGAGGGTACAACATTTACGATTCAATTTCCAGATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    888

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA2417 [new locus tag: SA_RS13860 ]
  • symbol: SA2417
  • description: hypothetical protein
  • length: 295
  • theoretical pI: 6.09533
  • theoretical MW: 34006
  • GRAVY: -0.00305085

Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 62.9)
    and 8 more
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 49.2)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 43.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 31.3)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 31.3)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 29.5)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 29.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA mismatch repair protein MutL (TIGR00585; HMM-score: 20.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase VI, B subunit (TIGR01052; EC 5.99.1.3; HMM-score: 17.5)
  • TheSEED  :
    • ABC transporter-like sensor linked histidine kinase SAV2624
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 73.1)
    and 11 more
    HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 26.9)
    HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 14.7)
    no clan defined DUF1043; Protein of unknown function (DUF1043) (PF06295; HMM-score: 13.9)
    Phage_holin_3_6; Putative Actinobacterial Holin-X, holin superfamily III (PF07332; HMM-score: 13.8)
    FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 13.4)
    His_Kinase_A (CL0025) HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 12.5)
    no clan defined DUF413; Protein of unknown function, DUF (PF04219; HMM-score: 11.9)
    DUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 11.8)
    NBD94; Nucleotide-Binding Domain 94 of RH (PF16830; HMM-score: 10.5)
    DUF3290; Protein of unknown function (DUF3290) (PF11694; HMM-score: 6.3)
    IncA; IncA protein (PF04156; HMM-score: 5.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000379
    • TAT(Tat/SPI): 0.000142
    • LIPO(Sec/SPII): 0.013345
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILLLLLIFIGIKYLSFVKTISQQQQIENLETALYQLKNEQIEYKNDVESYFLTWVHQMKTPITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIRPIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFDEQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPVSVVSKQNEGTTFTIQFPDE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]