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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0036 [new locus tag: SACOL_RS00200 ]
  • pan locus tag?: SAUPAN000107000
  • symbol: SACOL0036
  • pan gene symbol?:
  • synonym:
  • product: IS1272 transposase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0036 [new locus tag: SACOL_RS00200 ]
  • symbol: SACOL0036
  • product: IS1272 transposase
  • replicon: chromosome
  • strand: -
  • coordinates: 42958..44481
  • length: 1524
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATGTTAAAAGTGATTCTATATGCCTACACACAATCTGTATTCTCAGGTCGTAAAATA
    GAAAAAATGCTTAATGATAGCATCCGAATGATGTGGCTATCACAAAATCAAAAACCTTCT
    TATAAAACAATTAATCAATTTAGAGTAAATCCAAAAGTAGATGCTTTATTAGAATCTTTA
    TTTATTCAATTTTACAGTCAGTGTGTAAAACAAAATCTTATAGATGATAAAGCTATTTTT
    ATTGATGGTACAAAAATTGAAGCAAATGCCAATCGATATACATTTGTATGGAAAAAGAGT
    ATTCAAAACCATGAATCAAAGATGAATGAGGATTCTAAAGCCCTCTACCATGAATTGGTA
    ACCAATAAAATCATACCGGAAATTAAAGAAGATCATGATAATGAATTAACAAAAGAAGAA
    ATAGATTTGATTGGTAGTCACTTAGATAAAGAAATCGAAGATTTAAACCAACATATCAAC
    AATGAAAAATGTACTAAAACAAGAAAACAAATACGTCTCAAAAGAACTAAAATCAAAAAA
    TACAAAAAGCAAATCAATGATTATTTTGAGCGAAAGTATCGATACGAATTTCAAAAATCT
    ATTTTAAAGGATAGAAATAGTTATTCTAAGACAGATTATGATGCGACATTTATGAGAATG
    AAAGAAGATCACATGAAAAATGGACAACTTAAGCCAGGGTATAATTTACAAATAGCAACA
    AATTCCCAATTTGTTTTATCTTATAATGTGTATCAAAATCCAACGGATACTAGAACGATG
    ATTCCATTTTTAAATTCAATTCAAGAGACCTACGGTCATTTACCTGAATATATTGTAGCT
    GATGCAGGTTATGGTAGTGAATCAAATTATAAGGCAATTATAGATGACTTTAATCGAACG
    CCACTCATAACATATGGAATGTTTATAAAAGATAAAACTAAAAAATATAAAAGTGACATC
    TTTAATACTCAAAATTGGAACTATGACGAAATTAATGACGAATTCATTTGTCCGAATAAT
    AAACGGCTAGGTTTTAAAAGATATGCCTATCGTCATGATAAGTATGGTTATAAGCGAGAC
    TTCAAATTATATGAATGTGATGATTGTTCAGAATGTCCTCTGAAAAATCAATGTATGAAC
    TTCAATTCAAAAACAAACAAAAAAATAATGAAGAATTATAACTGGGAATATTTTAAATCC
    CAAATTAACAAAAAGCTTTCAGAACCAGAAACAAAAAATATCTACAGTCAAAGAAAAATT
    GATGTGGAACCTGTTTTTGGATTTATGAAGGCTATTTTGGGTTTCACTCGGATGTCTGTC
    CGAGGACTCAATAAAGTCAAAAGAGAACTTGGTTTTGTATTAATGGCACTTAATATAAGA
    AAAGTAGTAGCTCAACGAGCTGAAAATAATCAAAAAATTTATAAAAAAGACAATTTCTAT
    ATTATTTCAATAGAAATTGTCTTTTTTCACTTATCCAAGAACTTTATGTCCCGGACTCAT
    TATGATTTATTTTTTTATTGCTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1524

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0036 [new locus tag: SACOL_RS00200 ]
  • symbol: SACOL0036
  • description: IS1272 transposase
  • length: 507
  • theoretical pI: 9.74588
  • theoretical MW: 60733.3
  • GRAVY: -0.768245

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    putative transposase 
  • PFAM:
    RNase_H (CL0219) DDE_Tnp_1; Transposase DDE domain (PF01609; HMM-score: 129.9)
    DDE_Tnp_1_6; Transposase DDE domain (PF13751; HMM-score: 93.7)
    no clan definedDUF772; Transposase domain (DUF772) (PF05598; HMM-score: 40.9)
    UPF0179; Uncharacterised protein family (UPF0179) (PF03684; HMM-score: 10.8)
    Viral_ssRNA_CP (CL0055) Circo_capsid; Circovirus capsid protein (PF02443; HMM-score: 8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 0
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.155
    • Ymax_pos: 19
    • Cmax: 0.119
    • Cmax_pos: 19
    • Smax: 0.284
    • Smax_pos: 9
    • Smean: 0.21
    • D: 0.177
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MMLKVILYAYTQSVFSGRKIEKMLNDSIRMMWLSQNQKPSYKTINQFRVNPKVDALLESLFIQFYSQCVKQNLIDDKAIFIDGTKIEANANRYTFVWKKSIQNHESKMNEDSKALYHELVTNKIIPEIKEDHDNELTKEEIDLIGSHLDKEIEDLNQHINNEKCTKTRKQIRLKRTKIKKYKKQINDYFERKYRYEFQKSILKDRNSYSKTDYDATFMRMKEDHMKNGQLKPGYNLQIATNSQFVLSYNVYQNPTDTRTMIPFLNSIQETYGHLPEYIVADAGYGSESNYKAIIDDFNRTPLITYGMFIKDKTKKYKSDIFNTQNWNYDEINDEFICPNNKRLGFKRYAYRHDKYGYKRDFKLYECDDCSECPLKNQCMNFNSKTNKKIMKNYNWEYFKSQINKKLSEPETKNIYSQRKIDVEPVFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAENNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC

Experimental data[edit source | edit]

  • experimental localization: Cytoplasmic [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]