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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0046 [new locus tag: SACOL_RS00245 ]
  • pan locus tag?: SAUPAN000509000
  • symbol: SACOL0046
  • pan gene symbol?:
  • synonym:
  • product: metallo-beta-lactamase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0046 [new locus tag: SACOL_RS00245 ]
  • symbol: SACOL0046
  • product: metallo-beta-lactamase
  • replicon: chromosome
  • strand: -
  • coordinates: 53428..54756
  • length: 1329
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTTTTTTAAACAATTTTATGATAAACATTTATCTCAAGCATCTTATTTAATCGGTTGT
    CAAAAAACTGGAGAAGCCATGATTATTGATCCTATTCGTGACTTATCTTCATATATTCGA
    GTTGCTGATGAAGAAGGTTTAACCATTACTCATGCAGCTGAAACACATATACATGCAGAT
    TTTGCTTCAGGAATTAGAGATGTTGCTATAAAGTTAAATGCTAATATTTATGTATCGGGT
    GAAAGTGATGACACATTAGGTTATAAAAATATGCCTAACCACACTCATTTTGTTCAACAT
    AATGACGATATTTATGTAGGAAATATAAAATTAAAAGTGCTTCATACACCGGGTCACACG
    CCAGAAAGTATAAGTTTTTTACTTACTGACGAAGGTGCTGGAGCACAAGTTCCAATGGGA
    CTATTCAGTGGTGATTTTATTTTTGTAGGAGATATCGGTAGACCTGATTTACTAGAAAAA
    GCGGTTAAAGTAGAAGGATCATCTGAAATAGGCGCTAAACAAATGTTTAAATCGATTGAA
    AGTATTAAAGACTTGCCAGATTACATTCAAATTTGGCCTGGCCATGGCGCTGGTAGTCCT
    TGTGGTAAATCTTTAGGTGCTATTCCAACATCTACTCTTGGCTATGAAAAACAAACGAAC
    TGGGCTTTTTCTGAAAATAACGAAGCTACCTTTATCGATAAACTCATTTCTGACCAACCT
    GCACCACCACATCATTTTGCACAAATGAAAAAAATTAATCAATTCGGTATGAATTTATAT
    CAACCTTATACGGTTTATCCAGCTACAAATACAAACAGATTAACTTTTGATCTCCGCAGT
    AAGGAGGCTTATCATGGTGGACATATTGAAGGTACAATCAATATTCCATATGATAAAAAT
    TTCATCAATCAAATTGGCTGGTATCTAAACTATGATCAAGAAATTAACTTGATTGGAGAC
    TATCACCTTGTTTCAAAAGCAACACACACCTTACAACTCATTGGATACGATGATATTGCT
    GGATATCAATTACCGCAATCTAAGATTCAAACACGTTCCATTCATAGTGAAGACATTACA
    GGTAACGAATCACATATATTAGATGTACGTAATGATAATGAATGGAATAATGGCCACTTA
    TCTCAAGCGGTTCATGTACCACACGGCAAACTTTTAGAAACAGATTTACCTTTCAATAAA
    AACGATGTTATTTATGTACACTGTCAGTCTGGCATTAGAAGTTCGATAGCTATTGGTATT
    TTAGAACACAAAGGTTATCACAACATTATTAATGTAAATGAAGGTTATAAAGATATACAA
    CTTTCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1329

Protein[edit source | edit]

Protein Data Bank: 3R2U

General[edit source | edit]

  • locus tag: SACOL0046 [new locus tag: SACOL_RS00245 ]
  • symbol: SACOL0046
  • description: metallo-beta-lactamase
  • length: 442
  • theoretical pI: 5.97736
  • theoretical MW: 49410
  • GRAVY: -0.387104

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesDetoxificationhydroxyacylglutathione hydrolase (TIGR03413; EC 3.1.2.6; HMM-score: 74.5)
    phage shock operon rhodanese PspE (TIGR02981; EC 2.8.1.1; HMM-score: 37)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 22)
    thiazole biosynthesis domain (TIGR04271; HMM-score: 20.9)
  • TheSEED:  
    Zn-dependent hydroxyacylglutathione hydrolase / Polysulfide binding protein 
  • PFAM:
    Phosphatase (CL0031) Rhodanese; Rhodanese-like domain (PF00581; HMM-score: 66.3)
    Metallo-HOrase (CL0381) Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 29)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.83
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.127
    • Ymax_pos: 27
    • Cmax: 0.156
    • Cmax_pos: 53
    • Smax: 0.186
    • Smax_pos: 10
    • Smean: 0.122
    • D: 0.125
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MFFKQFYDKHLSQASYLIGCQKTGEAMIIDPIRDLSSYIRVADEEGLTITHAAETHIHADFASGIRDVAIKLNANIYVSGESDDTLGYKNMPNHTHFVQHNDDIYVGNIKLKVLHTPGHTPESISFLLTDEGAGAQVPMGLFSGDFIFVGDIGRPDLLEKAVKVEGSSEIGAKQMFKSIESIKDLPDYIQIWPGHGAGSPCGKSLGAIPTSTLGYEKQTNWAFSENNEATFIDKLISDQPAPPHHFAQMKKINQFGMNLYQPYTVYPATNTNRLTFDLRSKEAYHGGHIEGTINIPYDKNFINQIGWYLNYDQEINLIGDYHLVSKATHTLQLIGYDDIAGYQLPQSKIQTRSIHSEDITGNESHILDVRNDNEWNNGHLSQAVHVPHGKLLETDLPFNKNDVIYVHCQSGIRSSIAIGILEHKGYHNIINVNEGYKDIQLS

Experimental data[edit source | edit]

  • experimental localization: Lipoprotein [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]