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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0128 [new locus tag: SACOL_RS00645 ]
  • pan locus tag?: SAUPAN000957000
  • symbol: SACOL0128
  • pan gene symbol?: phnD
  • synonym:
  • product: phosphonate ABC transporter phosphonate-binding protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0128 [new locus tag: SACOL_RS00645 ]
  • symbol: SACOL0128
  • product: phosphonate ABC transporter phosphonate-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 143021..143977
  • length: 957
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAATTTTAAGTGTTTATTTGTATTAATGTTAGCAGTCATTGTTTTTGCAGCAGCA
    TGTGGAAACTCAAGTTCTTTAGATAATCAAAAGAACGCTAGTAATGATTCGGATTCTAAA
    TCAGGAGGATACAAACCTAAAGAATTAACCGTTCAATTTGTACCTTCGCAAAATGCTGGA
    ACATTAGAAGCTAAAGCAAAACCATTAGAAAAATTACTATCTAAAGAATTAGGGATTCCA
    GTTAAAGTGTCTGTATCAACTAACTACAATACAATTGTTGAAGCTATGAAGTCTAAAAAA
    GTTGATGTTGGTTTCTTACCACCAACGGCATACACATTAGCACATGATCAAAAAGCAGCT
    GATTTATTATTACAAGCACAACGTTTCGGTGTAAAAGAAGATGGTTCAGCAAGTAAAGAA
    CTTGTAGATAGTTATAAATCAGAAATTCTTGTTAAAAAAGACTCAAAAATTAAAAGCTTG
    AAAGATTTAAAAGGTAAGAAAATTGCCTTACAAGATGTAACATCAACTGCTGGATATACA
    TTCCCACTTGCGATGTTAAAAAACGAAGCAGGTATTAATGCAACTAAAGATATGAAAATT
    GTGAATGTTAAAGGTCATGACCAAGCAGTTATCTCATTATTAAATGGAGATGTAGATGCT
    GCGGCTGTATTTAACGATGCACGTAATACTGTGAAAAAAGACCAACCAAATGTATTTAAA
    GACACACGAATTTTAAAATTAACACAAGCTATTCCGAATGACACAATTTCTGTAAGACCA
    GATATGGATAAAGATTTTCAAGAAAAATTGAAAAAAGCTTTTATAGACATTGCTAAATCA
    AAAGAAGGTCACAAAATTATTAGCGAAGTTTATTCACATGAAGGATACACAGAAACGAAA
    GATTCAAATTTCGACATTGTAAGAGAGTACGAAAAATTAGTTAAAGATATGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    957

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0128 [new locus tag: SACOL_RS00645 ]
  • symbol: SACOL0128
  • description: phosphonate ABC transporter phosphonate-binding protein
  • length: 318
  • theoretical pI: 9.66321
  • theoretical MW: 35000
  • GRAVY: -0.393082

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAnionsphosphate/phosphite/phosphonate ABC transporter, periplasmic binding protein (TIGR01098; HMM-score: 257.2)
    phosphonate ABC transporter, periplasmic phosphonate binding protein (TIGR03431; HMM-score: 173.6)
    putative selenate ABC transporter periplasmic binding protein (TIGR04553; HMM-score: 76.2)
    MetabolismTransport and binding proteinsOtherABC transporter, substrate-binding protein, aliphatic sulfonates family (TIGR01728; HMM-score: 54.3)
    taurine ABC transporter, periplasmic binding protein (TIGR01729; HMM-score: 46.2)
    MetabolismTransport and binding proteinsUnknown substrateTRAP transporter solute receptor, TAXI family (TIGR02122; HMM-score: 37.2)
    MetabolismTransport and binding proteinsAmino acids, peptides and amineslysine-arginine-ornithine-binding periplasmic protein (TIGR01096; HMM-score: 22.2)
  • TheSEED:  
    Membrane TransportABC transportersABC transporter alkylphosphonate (TC 3.A.1.9.1) Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) 
  • PFAM:
    PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 241.7)
    NMT1; NMT1/THI5 like (PF09084; HMM-score: 48.4)
    SBP_bac_3; Bacterial extracellular solute-binding proteins, family 3 (PF00497; HMM-score: 44.1)
    NMT1_2; NMT1-like family (PF13379; HMM-score: 24.1)
    Mycoplasma_p37; High affinity transport system protein p37 (PF06646; HMM-score: 23.8)
    NMT1_3; NMT1-like family (PF16868; HMM-score: 21.3)
    Hexosaminidase (CL0546) Glyco_hydro_20b; Glycosyl hydrolase family 20, domain 2 (PF02838; HMM-score: 13.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: VFAAACG
  • SignalP: Signal peptide length 34 aa
    • Ymax: 0.398
    • Ymax_pos: 34
    • Cmax: 0.227
    • Cmax_pos: 34
    • Smax: 0.985
    • Smax_pos: 17
    • Smean: 0.856
    • D: 0.576
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGYKPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVDVGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSLKDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDVDAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFIDIAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]