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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0166 [new locus tag: SACOL_RS00845 ]
  • pan locus tag?: SAUPAN001020000
  • symbol: SACOL0166
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0166 [new locus tag: SACOL_RS00845 ]
  • symbol: SACOL0166
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 191118..191612
  • length: 495
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    TTGACGAAACGACAAATGGGTATATTCATTTATGCTGGAATTATCGGTGGCTTGTTATCT
    GGAATTGTAAAATTAGGTTGGGAGGTCATGTTTCCACCTCGCACACCAGAACGTAATGCA
    ACGAACCCACCTCAAGAGTTATTGCAACAATTAGGATTTAGTAGTGAGTTTACGCATCAA
    ACATATACATTTTCAAATATGGAATTGCCTTGGGTAAGCTTTATTGTCCACTTTAGTTTT
    TCTATCGTCATTGCAATTATTTACTGCATATTAGTTAAAAAATACGCTTACTTAGCAATG
    GGACAAGGTGCTGTTTTTGGTATTGCTATTTGGGTATTATTCCACCTTATCATTATGCCA
    ATCATGCATACTGTACCTGCTGTGTGGGATCAACCATTCCAAGAGCATCTGTCAGAATTC
    TTTGGCCATATCGTCTGGATGATGACAATTGAATTAGTGCGACAACATTTTGTCTATCGC
    TATAAATTAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    495

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0166 [new locus tag: SACOL_RS00845 ]
  • symbol: SACOL0166
  • description: hypothetical protein
  • length: 164
  • theoretical pI: 8.45954
  • theoretical MW: 19060.5
  • GRAVY: 0.464634

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01107837: hypothetical protein
  • PFAM:
    no clan defined DUF1440; Protein of unknown function (DUF1440) (PF07274; HMM-score: 198.8)
    and 3 more
    DUF1761; Protein of unknown function (DUF1761) (PF08570; HMM-score: 18.2)
    YqhR; Conserved membrane protein YqhR (PF11085; HMM-score: 17.1)
    PetN; PetN (PF03742; HMM-score: 11.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.287641
    • TAT(Tat/SPI): 0.050129
    • LIPO(Sec/SPII): 0.086381
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTKRQMGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQQLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFGIAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]