From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0168 [new locus tag: SACOL_RS00855 ]
  • pan locus tag?: SAUPAN001023000
  • symbol: argJ
  • pan gene symbol?: argJ
  • synonym:
  • product: bifunctional ornithine acetyltransferase/N-acetylglutamate synthase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0168 [new locus tag: SACOL_RS00855 ]
  • symbol: argJ
  • product: bifunctional ornithine acetyltransferase/N-acetylglutamate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 192647..193888
  • length: 1242
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAACATCAAGAAACGACATCACAACAATATAACTTTTCAATTATTAAACATGGCGAT
    ATCAGTACACCTCAAGGCTTCACGGCTGGTGGTATGCACATCGGTTTACGCGCTAACAAA
    AAAGACTTTGGGTGGATTTACTCATCGTCTTTGGCAAGTGCAGCTGCCGTATATACTTTA
    AATCAGTTTAAAGCTGCACCACTTATTGTCACTGAAGACACTTTACAAAAGTCTAAAGGA
    AAATTACAAGCACTTGTTGTTAATTCAGCTAATGCAAATTCTTGTACCGGTCAACAAGGC
    ATAGATGATGCACGACAAACACAAACATGGGTTGCTCAACAACTTCAAATACCATCTGAG
    CATGTTGCTGTTGCTTCAACTGGGGTCATTGGTGAATATTTGCCTATGGATAAAATTAAG
    ACTGGGACCGAACATATTAAGGATGCTAATTTTGCAACGCCAGGTGCGTTTAACGAGGCA
    ATTTTAACAACTGATACCTGTACAAAACATATCGCTGTATCACTAAAAATCGATGGTAAA
    ACCGTTACAATTGGTGGTAGCACCAAAGGTTCAGGTATGATTCACCCAAATATGGCTACC
    ATGCTTGCTTTTATAACAACCGATGCATCGATTGAATCGAATACACTTCATCAATTATTA
    AAATCTTCGACTGACCATACATTTAATATGATTACTGTTGATGGCGATACAAGTACAAAT
    GACATGGTATTAGTCATGGCAAATCACCAAGTTGAACACCAAATACTTAGTCAAGACCAT
    CCACAATGGGAAACATTTGTTGATGCATTCAATTTTGTCTGTACATTTTTAGCTAAAGCT
    ATAGCCAGAGATGGCGAAGGCGCAACAAAGTTAATCAGCGTCAATGTGTCAGGCGCTAAA
    TCAATATCAGATGCAAGAAAAATCGGTAAAACTATCGTAAGTTCAAATCTAGTAAAATCA
    GCTATTTTTGGCGAAGATGCCAATTTTGGTCGAATCATTACAGCTATTGGCTACAGCGGA
    TGTGAAATTGATCCTAACTGCACATATGTTCAACTGAACCAAATACCTGTCGTTGATAAA
    GGTATGGCTGTACTATTTGATGAGCAAGCTATGTCGAATACATTAACTCATGAAAATGTC
    ACAATTGACGTTCAGCTTGGTTTAGGTAACGCTGCAGCGACTGCATACGGTTGTGATTTA
    TCCTATGATTATGTGCGTATCAACGCATCATATCGAACATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1242

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0168 [new locus tag: SACOL_RS00855 ]
  • symbol: ArgJ
  • description: bifunctional ornithine acetyltransferase/N-acetylglutamate synthase
  • length: 413
  • theoretical pI: 6.18088
  • theoretical MW: 44399.8
  • GRAVY: -0.108232

Function[edit | edit source]

  • reaction:
    EC 2.3.1.1?  ExPASy
    Amino-acid N-acetyltransferase Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate
    EC 2.3.1.35?  ExPASy
    Glutamate N-acetyltransferase N2-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family glutamate N-acetyltransferase/amino-acid acetyltransferase (TIGR00120; EC 2.3.1.1,2.3.1.35; HMM-score: 494.5)
  • TheSEED  :
    • Glutamate N-acetyltransferase (EC 2.3.1.35)
    • N-acetylglutamate synthase (EC 2.3.1.1)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Glutamate N-acetyltransferase (EC 2.3.1.35)
    and 1 more
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  N-acetylglutamate synthase (EC 2.3.1.1)
  • PFAM:
    DmpA_ArgJ (CL0635) ArgJ; ArgJ family (PF01960; HMM-score: 481.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.67
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.027663
    • TAT(Tat/SPI): 0.010515
    • LIPO(Sec/SPII): 0.002982
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFGWIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGIDDARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEAILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQLLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTFLAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIITAIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAAATAYGCDLSYDYVRINASYRT

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: ArgR (repression) regulon
    ArgR(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]