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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0202 [new locus tag: SACOL_RS01030 ]
  • pan locus tag?: SAUPAN001077000
  • symbol: SACOL0202
  • pan gene symbol?: hptS
  • synonym:
  • product: sensor histidine kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0202 [new locus tag: SACOL_RS01030 ]
  • symbol: SACOL0202
  • product: sensor histidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 236712..238268
  • length: 1557
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGACGGCATACAAACCTTATAGACATCAATTAAGACGATCGTTATTTGCGTCTACTATT
    TTTCCAGTATTTTTAGTCATTATTATTGGACTAGTAAGCTTCTATGCCATCTATATTTGG
    ATTGAGCATCGTACGATTCATCAACATGTTGATGAAAGTCAGTCATCTTTACATCATACT
    GAAAAGCAGATTCAGACATTTATTACACAGCATAATAATTCGTTTCAAGAGCTCGATTTA
    ACAAATCATCATGATGTGACCGCTACAAAACGTGAATTATTAAAATTAATACACCAACAG
    CCCGCAACACTTTATTATGAATTATCTGGACCAAATCAGTTTATAACCAATAATTATGAA
    CATTTAAACACTAAAAATATGTATTTATTTTCAACGCATCAGCTTAAATTCAAAAATAGT
    ACATATATGCTTAAAATTTATATGGCTAACACCCCACGTTTAAGTGAAATTAAGAAAGAT
    AACCGACAATTCGCACTTATCGTTGATCAATATGACAATATTTTATACGCCAACGATGAC
    CGTTTTACTATAGGTGAAAAGTATAGACCACAACAATTCGGATTTATGAATGAATCTGTA
    AAATTAAACCATGCTGACCATCGTCTCATTATTTATAAAGATATACATGAAAACATTGAA
    GATGGTATCACGTTGTTAATTGTTATGGCCGTAGTTCTTGTTTTACTAGTAATATTTGGT
    TTCATTAGCGCAGATAATATGGCTAAACGACAAACAAAAGATATCGAAACGATTATTCAA
    AAAATTTACTATGCCAAAAATCGTCATCTAGGTACATATACGCCTTTAAAAAACAACAGT
    GAACTAGAAGAAATTAATAATTATATTTACGATTTATTTGAATCAAATGAGCAACTGATC
    CATTCTATCGAGCATACAGAACGTCGCTTGCGTGATATTCAGTTAAAGGAAATAGAACGC
    CAATTCCAGCCTCATTTCCTATTCAATACGATGCAAACAATACAATATTTAATTACGTTA
    TCTCCGAAATTAGCACAAACTGTGGTCCAACAATTATCGCAAATGTTACGTTACTCATTA
    CGTACAAACTCACATACAGTGGAATTAAACGAAGAGTTGAACTACATTGAACAGTATGTT
    GCAATACAAAACATCCGTTTTGATGACATGATTAAGCTCCATATTGAAAGTTCTGAAGAA
    GCGCGTCACCAGACAATTGGTAAAATGATGCTTCAGCCACTCATAGAAAATGCTATTAAA
    CATGGTAGAGATACTGAGTCATTGGACATTACTATACGCCTGACATTGGCACGTCAGAAT
    TTACATGTTTTAGTTTGCGACAATGGTATCGGGATGTCGTCATCTCGCCTGCAATATGTA
    CGCCAATCTCTTAATAATGATGTATTTGATACTAAGCATTTGGGATTAAATCATTTACAC
    AATAAAGCAATGATTCAATATGGTTCACATGCACGACTCCATATTTTTTCAAAGCGTAAT
    CAAGGTACGTTAATTTGTTATAAAATACCACTTTCAAGGGGGAATGTAGATGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1557

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0202 [new locus tag: SACOL_RS01030 ]
  • symbol: SACOL0202
  • description: sensor histidine kinase
  • length: 518
  • theoretical pI: 8.26122
  • theoretical MW: 61046.4
  • GRAVY: -0.383591

Function[edit source | edit]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinaseATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    Cellular processesCellular processesSporulation and germinationsporulation integral membrane protein YtvI (TIGR02872; HMM-score: 16.6)
    Cellular processesCellular processesSporulation and germinationanti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.4)
    Signal transductionRegulatory functionsProtein interactionsanti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.4)
    Signal transductionRegulatory functionsDNA interactionstranscriptional regulator, Spx/MgsR family (TIGR01617; HMM-score: 13.9)
    Cellular processesCellular processesSporulation and germinationstage II sporulation protein M (TIGR02831; HMM-score: 12.6)
    Cellular processesCellular processesCell divisioncell division protein FtsL (TIGR02209; HMM-score: 12.4)
  • TheSEED:  
    Two-component sensor histidine kinase 
  • PFAM:
    no clan definedHis_kinase; Histidine kinase (PF06580; HMM-score: 76)
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 25.8)
    no clan definedSIT; SHP2-interacting transmembrane adaptor protein, SIT (PF15330; HMM-score: 16.3)
    His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 14.5)
    no clan definedDUF2730; Protein of unknown function (DUF2730) (PF10805; HMM-score: 14.2)
    Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 14.1)
    BPD_transp_1 (CL0404) FtsX; FtsX-like permease family (PF02687; HMM-score: 13.9)
    no clan definedSpore_III_AB; Stage III sporulation protein AB (spore_III_AB) (PF09548; HMM-score: 13.7)
    Phage_barrel (CL0504) Phage-tail_3; Putative phage tail protein (PF13550; HMM-score: 13.1)
    no clan definedDUF4477; Domain of unknown function (DUF4477) (PF14780; HMM-score: 9.5)
    STAT_int; STAT protein, protein interaction domain (PF02865; HMM-score: 7.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.21
    • Ymax_pos: 11
    • Cmax: 0.118
    • Cmax_pos: 37
    • Smax: 0.492
    • Smax_pos: 1
    • Smean: 0.439
    • D: 0.299
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHRTIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPATLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKDNRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHENIEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPLKNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTIQYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIKLHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGIGMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYKIPLSRGNVDV

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Stephan Fuchs, Jan Pané-Farré, Christian Kohler, Michael Hecker, Susanne Engelmann
Anaerobic gene expression in Staphylococcus aureus.
J. Bacteriol.: 2007, 189(11);4275-89
[PubMed:17384184] [WorldCat.org] [DOI] (P p)