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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0213 [new locus tag: SACOL_RS01090 ]
  • pan locus tag?: SAUPAN001094000
  • symbol: SACOL0213
  • pan gene symbol?: fadD
  • synonym:
  • product: acyl-CoA dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0213 [new locus tag: SACOL_RS01090 ]
  • symbol: SACOL0213
  • product: acyl-CoA dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 251819..253030
  • length: 1212
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACATTTGAAAAAGAAACGGTCTTAAAAACATTATTTCCTGAAGATGTACTTAGTATT
    GCTAAAGGTTTAACAGACGGTGAAGTCGAATTTTTACAACAAGTAGATTCATTGCTAGAA
    AGTAAGTACCGTGAAAATATTAATCAACATTGGATAGACGCTACTGTACCCGAGGACTAT
    TTTAAAGATCTGGGAGAATTAAATTATTTTAACAATCCATTACTTTACAAGGATCGTCCA
    AACGCCAAAATGCCTAGTCAACTATTTCAGTTTTTCATGTCTTACCTACTCGCGCGATTT
    GATATTTCCTTAGCTACCCTACTCGGTGTTCACCAAGGTTTAGGGCATAACACTTTCTAT
    TTCGGAGGTAGCAAAGAACAAATTGCGAAATATGTACCTAAATTACAGTCACATGAACTG
    CGTACATGCTTTGCTTTAACTGAACCAGAACACGGTTCGGACGTTGCGGGAGGTCTTGAA
    ACAGTCGCTGAACGCCAAGGCGATACTTGGGTTATCAATGGTGAAAAGAAATGGATTGGT
    GGTGCACATGTATCTGATGTCATTCCAGTATTCGCAGTAAATAAAGAAACTGGCAAACCC
    CATTGCTTTGTAGTCAGACCAGAACAAGATGGCGTCGATATTGAAGTCATTGATAATAAA
    ATCGCACTTCGCATTGTTCCTAACGCCCTAATTAAATTAACTAATGTCAAAGTAGATGAA
    GCGGATCGCTTACAAAACATAACAAGCTTTAAAGATATTGCCAAAATTCTTTATTCAACG
    AGAGCAGGCGTTGCTTATATGGCTACAGGTGGTATGGCTGGCGCTTTACGTGCCACATTA
    GATTATGTCACTGAGCGTAAGCAATTCGGCAAACCAATTAGTAAATATCAGTTAATACAA
    GAAAAGCTAGCAATGATGCAAGGTAATTTAGCTCAAGCAATGGCAACATGTGCTCAATTA
    GCTAATATGCAAGCACATGGTGAATATGACGAGGTTGCAACTTCAACGGCGAAGATGATG
    AATGCCTTACGTTTGCGTGAGACAGTAGCTATGGGCCGCGGTATTACAGGTGGTAATGGC
    ATACTAGCTGACGATTATGATATTGCACGTTTCTTCTCTGATGCAGAAGCGATTTACACG
    TACGAAGGTACACATGAAATTAATGCCTTAGTAATTGGACGCGCTTTGACTGGAGATTCT
    GCTTTCGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1212

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0213 [new locus tag: SACOL_RS01090 ]
  • symbol: SACOL0213
  • description: acyl-CoA dehydrogenase
  • length: 403
  • theoretical pI: 5.17502
  • theoretical MW: 44728.7
  • GRAVY: -0.168983

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    cyclohexanecarboxyl-CoA dehydrogenase (TIGR03207; EC 1.1.1.-; HMM-score: 73)
    pimeloyl-CoA dehydrogenase, small subunit (TIGR03203; EC 1.3.1.62; HMM-score: 59.4)
    pimeloyl-CoA dehydrogenase, large subunit (TIGR03204; EC 1.3.1.62; HMM-score: 55.5)
    MetabolismCentral intermediary metabolismSulfur metabolismsulfur acquisition oxidoreductase, SfnB family (TIGR04022; EC 1.-.-.-; HMM-score: 35)
  • TheSEED:  
    Acyl-CoA dehydrogenase, long-chain specific (EC 1.3.8.8) 
  • PFAM:
    Acyl-CoA_dh (CL0087) Acyl-CoA_dh_1; Acyl-CoA dehydrogenase, C-terminal domain (PF00441; HMM-score: 93.9)
    no clan definedAcyl-CoA_dh_M; Acyl-CoA dehydrogenase, middle domain (PF02770; HMM-score: 69.6)
    Acyl-CoA_dh (CL0087) Acyl-CoA_dh_2; Acyl-CoA dehydrogenase, C-terminal domain (PF08028; HMM-score: 31.4)
    AcylCoA_ox_dh_N (CL0544) Acyl-CoA_dh_N; Acyl-CoA dehydrogenase, N-terminal domain (PF02771; HMM-score: 30.6)
    no clan definedDUF3726; Protein of unknown function (DUF3726) (PF12525; HMM-score: 13.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.117
    • Ymax_pos: 28
    • Cmax: 0.109
    • Cmax_pos: 24
    • Smax: 0.176
    • Smax_pos: 19
    • Smean: 0.112
    • D: 0.115
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYRENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDISLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLETVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVIDNKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGALRATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVATSTAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVIGRALTGDSAFV

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]