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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0346 [new locus tag: SACOL_RS01740 ]
  • pan locus tag?: SAUPAN001449000
  • symbol: SACOL0346
  • pan gene symbol?:
  • synonym:
  • product: prophage L54a, N-6-adenine-methyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0346 [new locus tag: SACOL_RS01740 ]
  • symbol: SACOL0346
  • product: prophage L54a, N-6-adenine-methyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 367591..368016
  • length: 426
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGGAAGTACATTACAGTAGTAAAACAAACGAGTGGACAACACCACAACATTTATTTGAT
    GACCTAAGCGAAGAATTCAGTTTTACATTAGATCCTTGTTCAACAGACGAGAACGCCAAA
    TGCCGGAAGTATTATACAGTAAAAGATAATGGGTTAATTCAAGACTGGTCTGAGGACATT
    GTTTTTATGAACCCGCCATACGGTCGAAGTATTAAGCGTTGGGTCAAGAAGGCTTACGAA
    GAAAGTTTGAAAGGCGCAACGGTAGTTTGTTTAATACCCGCAAGAACAGACACGACATAT
    TGGCATGATTACATTTTTAATAAGGCTGATGATATAAGATTCCTACGCGGTCGTCTGAAG
    TTTGGAGATAGTAAAAACAGCGCTCCTTTTCCTAGCGCAATTATCGTTTATAGAGGTGCA
    CAATGA
    60
    120
    180
    240
    300
    360
    420
    426

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0346 [new locus tag: SACOL_RS01740 ]
  • symbol: SACOL0346
  • description: prophage L54a, N-6-adenine-methyltransferase
  • length: 141
  • theoretical pI: 6.77433
  • theoretical MW: 16435.4
  • GRAVY: -0.634752

Function[edit source | edit]

  • reaction:
    EC 2.1.1.72?  ExPASy
    Site-specific DNA-methyltransferase (adenine-specific)S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
  • TIGRFAM:
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsphage N-6-adenine-methyltransferase (TIGR01712; EC 2.1.1.72; HMM-score: 96.4)
    Genetic information processingDNA metabolismRestriction/modificationphage N-6-adenine-methyltransferase (TIGR01712; EC 2.1.1.72; HMM-score: 96.4)
  • TheSEED:
  • PFAM:
    no clan definedDam; DNA N-6-adenine-methyltransferase (Dam) (PF05869; HMM-score: 112.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.139
    • Ymax_pos: 40
    • Cmax: 0.141
    • Cmax_pos: 40
    • Smax: 0.231
    • Smax_pos: 38
    • Smean: 0.094
    • D: 0.122
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MEVHYSSKTNEWTTPQHLFDDLSEEFSFTLDPCSTDENAKCRKYYTVKDNGLIQDWSEDIVFMNPPYGRSIKRWVKKAYEESLKGATVVCLIPARTDTTYWHDYIFNKADDIRFLRGRLKFGDSKNSAPFPSAIIVYRGAQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]