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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0416 [new locus tag: SACOL_RS02095 ]
  • pan locus tag?: SAUPAN001884000
  • symbol: SACOL0416
  • pan gene symbol?: fepC
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0416 [new locus tag: SACOL_RS02095 ]
  • symbol: SACOL0416
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 422006..423718
  • length: 1713
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    GTGAAACATTATTTGACTAAATTTGTAGCAATGCTAATAACTGCTGCTATGGTGTGTAGC
    TTTGGGTTACTGAAAAGTCAGGCAGCAGAACAACAAAGTATTAGTGATGTATATAGTGTG
    ATAACGGATGCGAAATCTGCACTTTCTAATAATTCGATATCGAATGACAATAAGCAGAAA
    GCAATTGAGCAAGTGGTAAGTGCAGTTAAGAAATTATCGCTTGAAGATAATAGTGAAAGT
    AATGCTGTCAAATCAGATGTGAGAAAGCTTGAAGATGCAAAAGCGAATGATAATCAAAAA
    GATACACTTTCGCAATTAACGAAGTCATTAATTGCTTATGAAGAGAAATTGGCTAGTAAA
    GATGCGGGTTCTAAAATTAAACTATTGCAACAGCAAGTCGATGCTAAAGATGCTGCGATG
    ACAAAAGCGATTAAAGATAAAAATAAAGCGGAATTAGAATCTTTGAACAATAGTTTGAAT
    CAGATTTGGACAAGTAATGAAACAGTGATTCGCAATTATGACGCAAATCAATATGGACAA
    ATTGAAGTCGCATTATTACAACTTAGAATTGCAATTCATAAGTCACCATTAGATACGGCA
    AAAGTGTCACATGCTTGGACAACTTTTAAATCAAATATTGATCATGTCGATAAAAAAAGT
    AATACGTCTGCAAATGATCAATACCATGTATCACAATTAAATGATGCGTTAGAGAAGGCG
    ATTAAAGCTATCGACGACAATCAATTGTCGGATGCTGATGCTGCGCTTACACATTTTATA
    GAAACTTGGCCGTATGTTGAAGGTCAAATTCAAACTAAAGACGGTGCTTTGTATACGAAA
    ATTGAAGATAAAATACCATATTATCAAAGTGTATTAGACGAACATAATAAAGCACATGTG
    AAAGATGGTTTAGTAGATTTAAATAACCAAATTAAAGAGGTTGTTGGCCATAGTTATAGC
    TTCGTCGATGTGATGATTATCTTTTTACGTGAAGGGCTAGAAGTGTTGTTAATTGTAATG
    ACATTGACTACCATGACGCGTAATGTAAAAGATAAGAAAGGGACTGCAAGTGTGATTGGT
    GGTGCAATTGCCGGACTTGTACTGAGTATTATCTTAGCAATTACGTTTGTAGAAACTTTA
    GGGAATAGTGGCATTCTTCGTGAAAGTATGGAAGCGGGATTAGGTATCGTTGCGGTCATA
    TTAATGTTTATCGTTGGTGTTTGGATGCACAAACGTTCAAATGCAAAACGTTGGAATGAC
    ATGATTAAAAATATGTATGCTAATGCGATTAGTAATGGTAATTTGGTATTGTTAGCGACG
    ATTGGTTTAATATCTGTGTTGCGTGAAGGTGTCGAGGTTATCATTTTCTATATGGGGATG
    ATAGGTGAGCTAGCGACCAAAGATTTTATTATTGGTATTGCTTTAGCTATCGTTATTTTA
    ATCATCTTTGCATTATTATTTAGATTTATAGTTAAATTAATACCTATTTTCTATATATTT
    AGAGTGTTGTCGATCTTTATTTTTATTATGGGATTCAAAATGCTTGGCGTAAGTATTCAA
    AAGTTACAATTATTAGGTGCGATGCCAAGACATGTTATTGAAGGATTCCCAACGATTAAC
    TGGTTGGGCTTTTATCCAAGTTATGAACCATTGATAGCACAAGGTGCTTATATTATGGTA
    GTTGCTATCTTAATCTTTAAATTTAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1713

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0416 [new locus tag: SACOL_RS02095 ]
  • symbol: SACOL0416
  • description: hypothetical protein
  • length: 570
  • theoretical pI: 8.66251
  • theoretical MW: 63307.4
  • GRAVY: 0.177018

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionGeneralFTR1 family protein (TIGR00145; HMM-score: 89.8)
  • TheSEED:  
    Iron acquisition and metabolismIron acquisition and metabolism - no subcategoryFerrous iron transporter EfeUOB, low-pH-induced Ferrous iron transport permease EfeU 
  • PFAM:
    MFS (CL0015) FTR1; Iron permease FTR1 family (PF03239; HMM-score: 71)
    no clan definedTim17; Tim17/Tim22/Tim23/Pmp24 family (PF02466; HMM-score: 14.4)
    MNHE; Na+/H+ ion antiporter subunit (PF01899; HMM-score: 9.8)
    CCDC84; Coiled coil protein 84 (PF14968; HMM-score: 6.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide length 29 aa
    • Ymax: 0.595
    • Ymax_pos: 29
    • Cmax: 0.408
    • Cmax_pos: 29
    • Smax: 0.946
    • Smax_pos: 14
    • Smean: 0.867
    • D: 0.701
  • predicted transmembrane helices (TMHMM): 7

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDAKSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDTLSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLNQIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDKKSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGALYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLEVLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEAGLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREGVEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIFIMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIFKFKK

Experimental data[edit source | edit]

  • experimental localization: Integral membrane [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SACOL1541 (Fur*)
    Fur* (TF) important in Iron homeostasisRegPrecise transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]