NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL0443 [new locus tag: SACOL_RS02235 ]
• pan locus tag^?: SAUPAN001970000
• symbol: SACOL0443
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL0443 [new locus tag: SACOL_RS02235 ]
• symbol: SACOL0443
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 446938..447306
• length: 369
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3236486 NCBI
• RefSeq: YP_185333 NCBI
• MicrobesOnline: 911913 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  
  TTGAAAACGATTTTAAAAACAATAACATATCTTGTACTTACTATCATTGGCGCTTATGCT
  GCTTTATTCATTTTAAAAACAATAGACTCCCATGGTATAACAGATCAATTTAACCCATTA
  GTAAAGAAGGATGATTCTTATGTTAAAACGACAGAGGTGTCTACTAGAATGGATGATCAA
  CTCCGAAGTTATAGTCAAAGTGTTTTTAATAAAGAAGGGAAAGAGACGCAATTAATGTAT
  ACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCAT
  GTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTACCAAAGAAAGCATTAGACAAACTG
  AGTCGATAA
  60
  120
  180
  240
  300
  360
  369
  

⊟Protein

⊟General

• locus tag: SACOL0443 [new locus tag: SACOL_RS02235 ]
• symbol: SACOL0443
• description: hypothetical protein
• length: 122
• theoretical pI: 9.26271
• theoretical MW: 14125.2
• GRAVY: -0.477869

⊟Function

• TIGRFAM:
  Hypothetical proteinsConservedconserved hypothetical protein (TIGR01655; HMM-score: 59.8)
• TheSEED:  
  FIG01108566: hypothetical protein 
• PFAM:
  no clan definedDUF1093; Protein of unknown function (DUF1093) (PF06486; HMM-score: 53.4)
  and 2 more
  Trigger_N; Bacterial trigger factor protein (TF) (PF05697; HMM-score: 16.3)
  Trigger_C (CL0262) SurA_N_3; SurA N-terminal domain (PF13624; HMM-score: 12.4)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular Possibility: 0.17
  • Signal Peptide Possibility: 0
  • N-terminally Anchored Score: 5
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.233
  • Ymax_pos: 21
  • Cmax: 0.218
  • Cmax_pos: 21
  • Smax: 0.479
  • Smax_pos: 1
  • Smean: 0.305
  • D: 0.261
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 57652630 NCBI
• RefSeq: YP_185333 NCBI
• UniProt: A0A0H2X038 UniProt

⊟Protein sequence

• MKTILKTITYLVLTIIGAYAALFILKTIDSHGITDQFNPLVKKDDSYVKTTEVSTRMDDQLRSYSQSVFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVETFEEVEKEEVPKKALDKLSR

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SACOL0443 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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