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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0563 [new locus tag: SACOL_RS02925 ]
  • pan locus tag?: SAUPAN002282000
  • symbol: SACOL0563
  • pan gene symbol?:
  • synonym:
  • product: GntR family transcriptional regulator

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0563 [new locus tag: SACOL_RS02925 ]
  • symbol: SACOL0563
  • product: GntR family transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 583479..584861
  • length: 1383
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    TTGAAAAACACTTTATACCATCAACTTTATGAAAAATTAAAAAAACAAATAATTGAAGGT
    CAATTTAAAGAAGGCGATAAATTTTATTCTAAAAGACAATTAAGCAAACATTTATCTATT
    AGCCAAACAACCGTAGAGCATGCATATCAACTTCTATTAGACGAAGGTTACATTTATTCA
    AGACCTCGCTCTGGATATTTTGTGTCCGAAATTGAATCTTTAACAATTTTAAACAATCAA
    CCTATCCCCTCTCTTTTTGATGATGATTCATATAAACCAAAAGCGAGTGATGAAGCTTAT
    GATTATGCTTTTAATTTGGATGAAATAGATACGAAACATTTCCCTATAGAACTTTTCAGA
    AAGTATTCCAAAGATCTTTATGATACAAATCATTTAAATCAATTACGTAGAGGACATTTT
    CAAGGAGAATTACATTTGCGGTTTCAACTGGCATTTTATTTATTTACTAATCGTGGCGTA
    ATTTGTGATCCTAATCAAATTATAATTGGCTCATCTACCGAACAATTAGTAAATCAATTA
    GTCGATTTACTTTATACTTCAACATTTATTATCGAAAAACCTAGTTATCCACCAATCAAA
    AATATTTTAGATAAAAAACAAGTTGAATATGAACAAATTGAAGTTGAAGATAATGGAATA
    AACGTAGATGAAGTTATTAAATCACAAAAAAATATAGTATATATAACGCCTTCCCATCAA
    TTTCCAACTGGATATGTGATGGACTTAAAAAAACGAACTCAATTAATACAATGGGCTCAA
    GAAAAAGAAGAACGATTTATTATAGAAGATGATTATGATTCCGAGTTTAGATATTTCGGC
    AAACCCATTCCAGCAATTCAAGGTCTATATTCAAGAGGAGAAAAAGTGATTTATATCAGT
    ACATTTTCAAAGTCTATTTTTCCTAGCTGTCGAGTTGCATATATGGTGTTACCCTATTCT
    ATAATGAAAAAATATCATTCTCAAAATCACATTGAAGGCAACACTGTACCTGTTCATATG
    CAAAACCTTATTGCAACGTTTATATCTTCCGGTGGTTTTGAAAGGCATTTAAATAAAATG
    AGACGAATTTATCGTAGAAAACTAACCTATATTTTAAAAAGATTGAAACCTTACAAAGAG
    CAGCTTGATATTCAAGGTGCTGAAACGGGTATGCATTTTACAATTACAGTAAAAAATGGA
    TTAACATTACAAGAGTGTCTTGATAGAGCAAACAAAGTAAAGTTAAAATTACAAGTTTAT
    AATTTTGATGATGATCAAGACTATAAGAAAACCCCTAAATTTATATTAGGCTTTGGCGGT
    ATAGACGATGATGCTTTAAAACCGCATGTAGACGCTCTAATAAAATCATTAATTATAAAG
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1383

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0563 [new locus tag: SACOL_RS02925 ]
  • symbol: SACOL0563
  • description: GntR family transcriptional regulator
  • length: 460
  • theoretical pI: 8.55168
  • theoretical MW: 53954.4
  • GRAVY: -0.510217

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    enduracididine biosynthesis enzyme MppQ (TIGR04461; EC 2.-.-.-; HMM-score: 87.9)
    succinyldiaminopimelate transaminase (TIGR03538; EC 2.6.1.17; HMM-score: 33.8)
    Signal transductionRegulatory functionsDNA interactionsphosphonate utilization transcriptional regulator PhnR (TIGR03337; HMM-score: 32.5)
    Signal transductionRegulatory functionsDNA interactionstrehalose operon repressor (TIGR02404; HMM-score: 31.9)
    MetabolismEnergy metabolismAmino acids and amineshistidine utilization repressor (TIGR02018; HMM-score: 29.9)
    Signal transductionRegulatory functionsDNA interactionshistidine utilization repressor (TIGR02018; HMM-score: 29.9)
    MetabolismTransport and binding proteinsAnionsphosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 24.4)
    Signal transductionRegulatory functionsDNA interactionsphosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 24.4)
    LL-diaminopimelate aminotransferase (TIGR03542; EC 2.6.1.83; HMM-score: 18.1)
    Signal transductionRegulatory functionsDNA interactionsbiotin operon repressor (TIGR00122; HMM-score: 16.6)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 16.1)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 15.5)
    glutaredoxin-like protein, YruB-family (TIGR02196; HMM-score: 13.7)
  • TheSEED:  
    Pyridoxin (Vitamin B6) Biosynthesis Transcriptional regulator of pyridoxine metabolism / Pyridoxamine phosphate aminotransferase (EC 2.6.1.54) 
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 45.7)
    HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 43.8)
    HTH_11; HTH domain (PF08279; HMM-score: 17.2)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 16)
    NUMOD1; NUMOD1 domain (PF07453; HMM-score: 15.3)
    RPA_C; Replication protein A C terminal (PF08784; HMM-score: 14.2)
    NADP_Rossmann (CL0063) MOLO1; Modulator of levamisole receptor-1 (PF17175; HMM-score: 12.5)
    HTH (CL0123) HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 9.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.131
    • Ymax_pos: 11
    • Cmax: 0.134
    • Cmax_pos: 49
    • Smax: 0.241
    • Smax_pos: 3
    • Smean: 0.171
    • D: 0.147
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTTVEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDYAFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGVICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDNGINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEFRYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGNTVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMHFTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHVDALIKSLIIK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL0563 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]