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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0573 [new locus tag: SACOL_RS02975 ]
  • pan locus tag?: SAUPAN002293000
  • symbol: SACOL0573
  • pan gene symbol?:
  • synonym:
  • product: PIN/TRAM domain-containing protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0573 [new locus tag: SACOL_RS02975 ]
  • symbol: SACOL0573
  • product: PIN/TRAM domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 594384..595457
  • length: 1074
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    GTGAATATCGTTAAACTAATGGTTATTATTATTTACTTAATTATTGGGAGCGCATTAGGA
    ATAATTATTATTCCTGAAATTGCAAATGATCTTGGATTACAAAACTCCAGCTTTTTAAAA
    AATCACTATGTAGATGGCATTATCGGTAGTATTTTTATGTTCTTAATTTTTGGTGTATTT
    ATTAGACGAGTTACTAACGCTATAAAAGGTTTAGAACATTTTATTATGCGTAGAAGTGCT
    GTTGAAATACTATTCGCAACAATAGGTTTAATAATCGGATTACTTATTTCTGTTATGGTG
    TCGTTTATATTAGAATCAATTGGCAACTCTATTTTTAATCATTTCATTCCTGTCATAATT
    ACGATATTACTATGTTATTTCGGTTTCCAATTTGGCCTTAAAAAACGAGATGAAATGTTA
    ATGTTTTTACCTGAGAATATAGCGCGTTCCATGTCACAACATACTAAAAGTGCTACGCCA
    AAAATTATCGACACAAGCGCAATTATTGATGGTCGTATTTTAGAAGTCATTCGTTGCGGT
    TTTATCGATGGCAATATTTTAATTCCACAAGGTGTTATTAATGAATTACAAATTGTTGCA
    GATTCAAATGACAGTGTTAAACGTGAAAAGGGTAAAAGAGGCTTAGATATTTTAAATGAA
    TTGTATGATTTAGACTATCCTACAAAGGTTATACATCCAACTAAAACACATAGTGATATT
    GATACGATGTTATTAAAATTAGCAAAACAATATCATGCAAGTATTATAACGACAGATTTC
    AACCTAAATAAAGTTTGTCATGTACATGGTATCAAAGCATTAAATGTTAATGATTTATCA
    GAAGCAATCAAACCTAATGTACATCAAGGTGATCAACTGCATATTTTACTGACAAAAATG
    GGTAAGGAGCCTGGTCAGGCAGTAGGATATCTAGATGATGGTACGATGGTAGTTGTTGAT
    AATGCTAAAAATCTTATTGGCAGTCATGTCAATTTAGAAGTAGTCAGCTTATTGCAAACA
    TCTTCAGGAAGAATTGTTTTTGCTAAAAAAATCGAAGATACAGTATCATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1074

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0573 [new locus tag: SACOL_RS02975 ]
  • symbol: SACOL0573
  • description: PIN/TRAM domain-containing protein
  • length: 357
  • theoretical pI: 7.4154
  • theoretical MW: 39586.4
  • GRAVY: 0.413165

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    RNA ligase partner, MJ_0950 family (TIGR03875; HMM-score: 15.4)
    MetabolismEnergy metabolismOthertetrahydromethanopterin S-methyltransferase, subunit C (TIGR01148; EC 2.1.1.86; HMM-score: 11.2)
  • TheSEED:  
    Stress ResponseHeat shockHeat shock dnaK gene cluster extended Membrane-associated protein containing RNA-binding TRAM domain and ribonuclease PIN-domain, YacL B.subtilis ortholog 
  • PFAM:
    OB (CL0021) TRAM; TRAM domain (PF01938; HMM-score: 17.6)
    PIN (CL0280) PIN; PIN domain (PF01850; HMM-score: 16.5)
    PIN_5; PINc domain ribonuclease (PF08745; HMM-score: 14.8)
    LysE (CL0292) DsbD_2; Cytochrome C biogenesis protein transmembrane region (PF13386; HMM-score: 13.4)
    no clan definedMtrC; Tetrahydromethanopterin S-methyltransferase, subunit C (PF04211; HMM-score: 12)
    LysE (CL0292) Colicin_V; Colicin V production protein (PF02674; HMM-score: 10)
    no clan definedVirul_fac_BrkB; Virulence factor BrkB (PF03631; HMM-score: 6.6)
    DUF4514; Domain of unknown function (DUF4514) (PF14986; HMM-score: 6.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.07
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.18
    • Extracellular Score: 0.21
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.172
    • Ymax_pos: 31
    • Cmax: 0.162
    • Cmax_pos: 31
    • Smax: 0.234
    • Smax_pos: 4
    • Smean: 0.174
    • D: 0.172
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYVDGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSFILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATPKIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDILNELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNVNDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEVVSLLQTSSGRIVFAKKIEDTVSL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]