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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0620 [new locus tag: SACOL_RS03205 ]
  • pan locus tag?: SAUPAN002350000
  • symbol: proP
  • pan gene symbol?: proP
  • synonym:
  • product: osmoprotectant proline transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0620 [new locus tag: SACOL_RS03205 ]
  • symbol: proP
  • product: osmoprotectant proline transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 652802..654202
  • length: 1401
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGATTTTAATAAAGAGAATATTAACATGGTGGATGCAAAGAAAGCTAAAAAAACCGTT
    GTTGCAACCGGTATCGGTAATGCAATGGAATGGTTCGATTTTGGTGTCTATGCATATACA
    ACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATG
    TTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTT
    GGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATG
    GCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCA
    CCAATACTATTATTGCTTGCAAGAGTACTACAAGGGTTTTCAACAGGTGGAGAGTATGCG
    GGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGT
    GGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTA
    ACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTC
    GGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTT
    TTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATC
    AGATTTTATTACAAAGATATATTTGTATGTTTTGTAGCTGTTGTATTCTTCAATGTTACA
    AACTATATGGTAACTGCATATTTACCAACCTATTTAGAACAAGTTATTAAATTAGATGCA
    ACGACAACAAGTGTATTAATTACTTGTGTCATGGCAATAATGATTCCATTAGCATTAATG
    TTTGGTAAGTTAGCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGG
    CTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATA
    GTAATCGGTATATTTATATTAGGATTTTTCTTATCAACTTACGAAGCGACAATGCCAGGG
    TCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAAT
    ATCTCTGTTTCGATATTTGGTGGTACGACGCCATTAGTTGCAACATGGTTAGTTACGAAA
    ACTGGAGATCCATTAGCACCTGCGTATTATTTAACAGCAATCAGTGTTATTGGCTTTTTA
    GTTATTACATTCTTACATTTAAGTACAGCAGGAAAATCTCTAAAAGGTTCGTATCCAAAT
    GTAGATAACGAGCAAGATAGAGCTTATTATGCAGAACATCCAAAAGAAGCATTATGGTGG
    GTTAAAGAACGTAAGAATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1401

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0620 [new locus tag: SACOL_RS03205 ]
  • symbol: ProP
  • description: osmoprotectant proline transporter
  • length: 466
  • theoretical pI: 8.48542
  • theoretical MW: 51657.3
  • GRAVY: 0.524678

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsUnknown substrateMFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 383.6)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 105)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 78)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsphosphoglycerate transporter family protein (TIGR00881; HMM-score: 51.2)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsD-galactonate transporter (TIGR00893; HMM-score: 49.3)
    MetabolismTransport and binding proteinsCations and iron carrying compoundscation transport protein (TIGR00898; HMM-score: 44.9)
    multidrug resistance protein (TIGR00880; HMM-score: 42.1)
    MetabolismTransport and binding proteinsAnionsphosphate:H+ symporter (TIGR00887; HMM-score: 35.2)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, sugar porter (SP) family (TIGR00879; HMM-score: 32.3)
    MetabolismTransport and binding proteinsAnionsNa(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 28.1)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 23.9)
    synaptic vesicle protein SV2 (TIGR01299; HMM-score: 22.3)
    Cellular processesCellular processesToxin production and resistancedrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 20.7)
    MetabolismTransport and binding proteinsOtherdrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 20.7)
  • TheSEED:  
    Amino Acids and DerivativesProline and 4-hydroxyprolineProline, 4-hydroxyproline uptake and utilization L-Proline/Glycine betaine transporter ProP 
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 114.4)
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 108.1)
    MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 36.7)
    no clan definedProminin; Prominin (PF05478; HMM-score: 4.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.196
    • Ymax_pos: 16
    • Cmax: 0.19
    • Cmax_pos: 39
    • Smax: 0.496
    • Smax_pos: 10
    • Smean: 0.341
    • D: 0.252
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYIGANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAFSTLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGSGLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEESPVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVIKLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHSQSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPLVATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYYAEHPKEALWWVKERKN

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: RpoF (SigB*) [3] other strains
    RpoF  (SigB*) (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int. J. Med. Microbiol.: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]