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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0654 [new locus tag: SACOL_RS03385 ]
  • pan locus tag?: SAUPAN002445000
  • symbol: SACOL0654
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0654 [new locus tag: SACOL_RS03385 ]
  • symbol: SACOL0654
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 683334..683960
  • length: 627
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAATTATTAAATAT
    AATGATGAATACTTAATGGTCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTT
    ATTAATTGGTTCATCCCAAAAGAATACGTCAAAATTAGTAGAGAAGAGTTTGAAAACTTA
    AATATTGTTAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTAGCTCTGCTTTG
    CTGGGAGTTGCATTAAGAAAATATACACATTTACTTGACATTCAACTTGATAAAAAATTA
    GTTATTGCCATATGTTGCATCACATTTATAGGGATTTTAATATTTTATGTACGCCTAATT
    AAAAAATCATCTTTAAATATTTATAATACTAAAAATAAAAGGTCAAAAATTTTTTTAATA
    CCTACACTAAAAAATGTTTGTTTCACATTATTTGGATATATTTTATTTGGCGGATTGACT
    ATGCTATTCCTAGATGCACTATTATCAATGAGTTATCAAAACATAATAGTATATTTTGTT
    TGGATTGCAGTTATAATGGGTTTTTTTCTAGTTAATATAGCTTTAATTATAGATAAAAAC
    ATTCATGTCATACTTAAAAACCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    627

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0654 [new locus tag: SACOL_RS03385 ]
  • symbol: SACOL0654
  • description: hypothetical protein
  • length: 208
  • theoretical pI: 10.0825
  • theoretical MW: 24278.2
  • GRAVY: 0.622596

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    tandem five-TM protein (TIGR01218; HMM-score: 173.5)
  • TheSEED:  
    repetative hypothetical protein , fig|282458.1.peg.574 homolog 
  • PFAM:
    no clan definedDUF443; Protein of unknown function (DUF443) (PF04276; HMM-score: 228.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.125
    • Ymax_pos: 12
    • Cmax: 0.113
    • Cmax_pos: 47
    • Smax: 0.195
    • Smax_pos: 4
    • Smean: 0.153
    • D: 0.136
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLLCESKIINKNPKYRIIKYNDEYLMVDIISTWISLFFPFINWFIPKEYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTHLLDIQLDKKLVIAICCITFIGILIFYVRLIKKSSLNIYNTKNKRSKIFLIPTLKNVCFTLFGYILFGGLTMLFLDALLSMSYQNIIVYFVWIAVIMGFFLVNIALIIDKNIHVILKNQ

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]