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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0832 [new locus tag: SACOL_RS04280 ]
  • pan locus tag?: SAUPAN002692000
  • symbol: SACOL0832
  • pan gene symbol?: whiA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0832 [new locus tag: SACOL_RS04280 ]
  • symbol: SACOL0832
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 858434..859378
  • length: 945
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGCTTTGCATCAGAAATGAAAAATGAATTAACTAGAATAGACGTCGATGAAATGAAT
    GCAAAAGCAGAGCTCAGTGCACTGATTCGAATGAATGGTGCACTTAGTCTTTCAAATCAA
    CAATTTGTTATAAATGTTCAAACGGAAAATGCAACAACGGCAAGACGTATTTATTCGTTG
    ATTAAACGTGTCTTTAATGTGGAAGTTGAAATATTAGTCCGTAAAAAAATGAAACTTAAA
    AAAAATAATATTTATATTTGTCGTACAAAGATGAAAGCGAAAGAAATTCTTGATGAATTA
    GGAATTTTAAAAGACGGCATTTTTACGCATGAAATTGATCATTCAATGATTCAAGATGAC
    GAAATGAGACGCAGTTACTTGAGAGGAGCTTTTCTGGCAGGTGGCTCAGTGAATAACCCT
    GAAACATCTTCGTACCATTTGGAAATTTTTTCTCAAAATGAGAGTCATGCAGAAGGCTTA
    ACGAAACTAATGAATAGTTATGAGTTGAATGCCAAACATTTAGAGCGAAAAAAAGGAAGT
    ATTACGTATTTAAAAGAAGCGGAAAAGATTTCGGATTTTCTTAGTTTGATAGGTGGCTAT
    CAAGCGTTATTAAAATTTGAAGACGTACGTATTGTAAGAGATATGCGTAATTCTGTTAAC
    CGACTCGTTAATTGTGAAACGGCCAATCTAAATAAAACAGTTAGTGCTGCGATGAAACAA
    GTTGAGAGCATTAAATTGATTGATAAAGAAATTGGTATTGAAAATTTACCAGACAGGTTG
    AGAGAGATTGCTAGAATTCGAGTAGAACATCAAGAAATTTCGTTGAAAGAGCTTGGAGAA
    ATGGTATCAACTGGTCCAATTTCAAAATCAGGTGTAAATCACCGATTAAGAAAACTGAAT
    GATTTAGCCGATAAGATTAGAAATGGTGAACAAATAGAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    945

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0832 [new locus tag: SACOL_RS04280 ]
  • symbol: SACOL0832
  • description: hypothetical protein
  • length: 314
  • theoretical pI: 9.22195
  • theoretical MW: 35868.1
  • GRAVY: -0.422611

Function[edit source | edit]

  • TIGRFAM:
    Cellular processesCellular processesCell divisionDNA-binding protein WhiA (TIGR00647; HMM-score: 447.1)
  • TheSEED:  
    Cytoplasmic hypothetical protein DUF199 
  • PFAM:
    Homing_endonuc (CL0324) LAGLIDADG_WhiA; WhiA LAGLIDADG-like domain (PF14527; HMM-score: 125.3)
    HTH (CL0123) HTH_WhiA; WhiA C-terminal HTH domain (PF02650; HMM-score: 121.2)
    Homing_endonuc (CL0324) WhiA_N; WhiA N-terminal LAGLIDADG-like domain (PF10298; HMM-score: 118.2)
    and 2 more
    LAGLIDADG_3; LAGLIDADG-like domain (PF14528; HMM-score: 16.7)
    no clan definedDUF3486; Protein of unknown function (DUF3486) (PF11985; HMM-score: 14.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.16
    • Ymax_pos: 47
    • Cmax: 0.142
    • Cmax_pos: 52
    • Smax: 0.281
    • Smax_pos: 43
    • Smean: 0.132
    • D: 0.149
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVINVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGILKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGLTKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNSVNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLKELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2]
    quantitative data / protein copy number per cell: 48 [3]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: RpoF (activation) regulon (SigB*) [4] other strains
    RpoF  (SigB*)(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  4. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]

H De Lencastre, S W Wu, M G Pinho, A M Ludovice, S Filipe, S Gardete, R Sobral, S Gill, M Chung, A Tomasz
Antibiotic resistance as a stress response: complete sequencing of a large number of chromosomal loci in Staphylococcus aureus strain COL that impact on the expression of resistance to methicillin.
Microb. Drug Resist.: 1999, 5(3);163-75
[PubMed:10566865] [WorldCat.org] [DOI] (P p)