From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0855 [new locus tag: SACOL_RS04390 ]
  • pan locus tag?: SAUPAN002778000
  • symbol: SACOL0855
  • pan gene symbol?:
  • synonym:
  • product: acetyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0855 [new locus tag: SACOL_RS04390 ]
  • symbol: SACOL0855
  • product: acetyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 879850..880380
  • length: 531
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGCAAATTAGACAAATACATCAACATGACTTTGCTCAAGTGGACCAGTTAATTAGAACG
    GCATTTGAAAATAGTGAACATGGTTATGGTAATGAATCAGAGCTAGTAGACCAAATTCGT
    CTAAGTGATACGTATGACAATACCTTAGAATTAGTAGCTGTTCTTCAAAATGAAGTTGTA
    GGGCACGGTTTACTAAGTGAAGTTTATCTTGATAACGAGGCACAACGGGAAATTGGATTA
    GTGTTAGCACCTGTATCTGTTGATATTCATCATCAAAATAAAGGTATTGGGAAGCGATTG
    ATTCAAGCATTAGAACGAGAAGCAATATTAAAAGGATATAATTTTATCAGTGTATTAGGA
    TGGCCGACGTATTATGCCAATCTAGGATATCAACGCGCAAGTATGTACGACATTTATCCA
    CCATATGATGGTATACCAGACGAAGCGTTTTTAATTAAAGAATTAAAAGTGAACAGTTTA
    GCGGGAAAAACAGGTACCATAAATTACACATCTGCTTTTGAAAAAATATGA
    60
    120
    180
    240
    300
    360
    420
    480
    531

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0855 [new locus tag: SACOL_RS04390 ]
  • symbol: SACOL0855
  • description: acetyltransferase
  • length: 176
  • theoretical pI: 4.71943
  • theoretical MW: 19928.3
  • GRAVY: -0.264205

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingTranscriptionDNA-dependent RNA polymeraseradical SAM enzyme/protein acetyltransferase, ELP3 family (TIGR01211; EC 2.3.1.48; HMM-score: 15)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationribosomal-protein-alanine acetyltransferase (TIGR01575; EC 2.3.1.128; HMM-score: 13.7)
  • TheSEED:  
    FIG01108786: hypothetical protein 
  • PFAM:
    Acetyltrans (CL0257) Acetyltransf_1; Acetyltransferase (GNAT) family (PF00583; HMM-score: 34.3)
    Acetyltransf_7; Acetyltransferase (GNAT) domain (PF13508; HMM-score: 27.5)
    Acetyltransf_9; Acetyltransferase (GNAT) domain (PF13527; HMM-score: 24.1)
    Acetyltransf_10; Acetyltransferase (GNAT) domain (PF13673; HMM-score: 18.2)
    FR47; FR47-like protein (PF08445; HMM-score: 14.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.106
    • Ymax_pos: 31
    • Cmax: 0.114
    • Cmax_pos: 31
    • Smax: 0.12
    • Smax_pos: 26
    • Smean: 0.079
    • D: 0.095
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDTYDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSLAGKTGTINYTSAFEKI

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2]
    quantitative data / protein copy number per cell: 118 [3]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: RpoF (SigB*) [4] other strains
    RpoF  (SigB*) (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  4. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]