From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0858 [new locus tag: SACOL_RS04405 ]
  • pan locus tag?: SAUPAN002796000
  • symbol: empbp
  • pan gene symbol?: emp
  • synonym:
  • product: secretory extracellular matrix and plasma binding protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0858 [new locus tag: SACOL_RS04405 ]
  • symbol: empbp
  • product: secretory extracellular matrix and plasma binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 885542..886564
  • length: 1023
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAAAGAAATTATTAGTTTTAACTATGAGCACGCTATTTGCTACACAAATTATGAAT
    TCAAATCACGCTAAAGCATCAGTGACAGAGAGTGTTGACAAAAAATTTGTAGTTCCAGAA
    TCAGGAATTAATAAAATTATTCCAGCTTACGATGAATTTAAGAATTCGCCAAAAGTAAAT
    GTTAGTAATTTAACTGACAATAAAAACTTTGTAGCTTCTGAAGATAAATTGAATAAGATT
    GCAGATTCATCGGCAGCTAGTAAAATTGTAGATAAAAACTTTGTCGTACCAGAATCAAAG
    TTAGGAAACATTGTGCCAGAGTACAAAGAAATCAATAATCGCGTGAATGTAGCAACAAAC
    AATCCAGCTTCACAACAAGTTGATAAGCATTTTGTTGCTAAAGGCCCAGAAGTAAATAGA
    TTTATTACGCAAAACAAAGTAAACCACCACTTCATTACTACGCAAACCCACTACAAGAAA
    GTTATTACTTCATACAAATCAACACATGTACATAAACATGTAAATCATGCAAAGGATTCT
    ATTAATAAACACTTTATTGTTAAACCATCAGAATCGCCTAGATATACACATCCATCTCAA
    TCTTTAATTATCAAGCATCATTTTGCAGTTCCTGGATATCACGCGCATAAATTTGTTACA
    CCAGGGCATGCTAGCATTAAAATTAATCACTTTTGTGTTGTGCCACAAATAAATAGTTTC
    AAGGTAATTCCACCATATGGTCACAATTCACATCGTATGCATGTACCAAGTTTCCAAAAT
    AACACAACAGCAACACATCAAAATGCTAAAGTAAATAAAGCATATGACTATAAATACTTC
    TATTCTTATAAAGTAGTTAAAGGTGTGAAGAAATATTTCTCATTTTCACAATCAAATGGT
    TATAAAATTGGGAAACCATCATTAAATATCAAAAATGTAAATTATCAATATGCTGTTCCA
    AGTTATAGCCCTACACACTACGTTCCTGAATTTAAGGGTAGCTTACCAGCACCACGAGTA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1023

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0858 [new locus tag: SACOL_RS04405 ]
  • symbol: Empbp
  • description: secretory extracellular matrix and plasma binding protein
  • length: 340
  • theoretical pI: 10.4459
  • theoretical MW: 38484.6
  • GRAVY: -0.56

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Extracellular ECM and plasma binding protein Emp
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Extracellular ECM and plasma binding protein Emp
  • PFAM:
    no clan defined Paramyxo_PCT; Phosphoprotein P region PCT disordered (PF14320; HMM-score: 11.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: NHAKASVT
  • SignalP: Signal peptide SP(Sec/SPI) length 26 aa
    • SP(Sec/SPI): 0.99514
    • TAT(Tat/SPI): 0.000591
    • LIPO(Sec/SPII): 0.003019
    • Cleavage Site: CS pos: 26-27. AKA-SV. Pr: 0.9437
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGINKIIPAYDEFKNSPKVNVSNLTDNKNFVASEDKLNKIADSSAASKIVDKNFVVPESKLGNIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKKVITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKFVTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYDYKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGSLPAPRV

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cell wall associated [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]

M Hussain, K Becker, C von Eiff, J Schrenzel, G Peters, M Herrmann
Identification and characterization of a novel 38.5-kilodalton cell surface protein of Staphylococcus aureus with extended-spectrum binding activity for extracellular matrix and plasma proteins.
J Bacteriol: 2001, 183(23);6778-86
[PubMed:11698365] [WorldCat.org] [DOI] (P p)